Flow Cytometry: A Blessing and a Curse
Flow cytometry is a laser-based technology generating a scattered and a fluorescent light signal that enables rapid analysis of the size and granularity of a particle or single cell. In addition, it offers the opportunity to phenotypically characterize and collect the cell with the use of a variety...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/9/11/1613 |
| _version_ | 1797511126759505920 |
|---|---|
| author | Hannah Drescher Sabine Weiskirchen Ralf Weiskirchen |
| author_facet | Hannah Drescher Sabine Weiskirchen Ralf Weiskirchen |
| author_sort | Hannah Drescher |
| collection | DOAJ |
| description | Flow cytometry is a laser-based technology generating a scattered and a fluorescent light signal that enables rapid analysis of the size and granularity of a particle or single cell. In addition, it offers the opportunity to phenotypically characterize and collect the cell with the use of a variety of fluorescent reagents. These reagents include but are not limited to fluorochrome-conjugated antibodies, fluorescent expressing protein-, viability-, and DNA-binding dyes. Major developments in reagents, electronics, and software within the last 30 years have greatly expanded the ability to combine up to 50 antibodies in one single tube. However, these advances also harbor technical risks and interpretation issues in the identification of certain cell populations which will be summarized in this viewpoint article. It will further provide an overview of different potential applications of flow cytometry in research and its possibilities to be used in the clinic. |
| first_indexed | 2024-03-10T05:41:02Z |
| format | Article |
| id | doaj.art-39528bdbf9f1489db0c3ef99247753dc |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T05:41:02Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-39528bdbf9f1489db0c3ef99247753dc2023-11-22T22:30:48ZengMDPI AGBiomedicines2227-90592021-11-01911161310.3390/biomedicines9111613Flow Cytometry: A Blessing and a CurseHannah Drescher0Sabine Weiskirchen1Ralf Weiskirchen2Division of Gastroenterology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USAInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, GermanyInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, GermanyFlow cytometry is a laser-based technology generating a scattered and a fluorescent light signal that enables rapid analysis of the size and granularity of a particle or single cell. In addition, it offers the opportunity to phenotypically characterize and collect the cell with the use of a variety of fluorescent reagents. These reagents include but are not limited to fluorochrome-conjugated antibodies, fluorescent expressing protein-, viability-, and DNA-binding dyes. Major developments in reagents, electronics, and software within the last 30 years have greatly expanded the ability to combine up to 50 antibodies in one single tube. However, these advances also harbor technical risks and interpretation issues in the identification of certain cell populations which will be summarized in this viewpoint article. It will further provide an overview of different potential applications of flow cytometry in research and its possibilities to be used in the clinic.https://www.mdpi.com/2227-9059/9/11/1613flow cytometrymultiparameter analysisimmunophenotyping |
| spellingShingle | Hannah Drescher Sabine Weiskirchen Ralf Weiskirchen Flow Cytometry: A Blessing and a Curse Biomedicines flow cytometry multiparameter analysis immunophenotyping |
| title | Flow Cytometry: A Blessing and a Curse |
| title_full | Flow Cytometry: A Blessing and a Curse |
| title_fullStr | Flow Cytometry: A Blessing and a Curse |
| title_full_unstemmed | Flow Cytometry: A Blessing and a Curse |
| title_short | Flow Cytometry: A Blessing and a Curse |
| title_sort | flow cytometry a blessing and a curse |
| topic | flow cytometry multiparameter analysis immunophenotyping |
| url | https://www.mdpi.com/2227-9059/9/11/1613 |
| work_keys_str_mv | AT hannahdrescher flowcytometryablessingandacurse AT sabineweiskirchen flowcytometryablessingandacurse AT ralfweiskirchen flowcytometryablessingandacurse |