Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms
Living organisms have evolved over millions of years to fine tune their metabolism to create efficient pathways for producing metabolites necessary for their survival. Advancement in the field of synthetic biology has enabled the exploitation of these metabolic pathways for the production of desired...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00155/full |
| _version_ | 1818840894915215360 |
|---|---|
| author | Gazi Sakir Hossain Gazi Sakir Hossain Saravanan Prabhu Nadarajan Saravanan Prabhu Nadarajan Lei Zhang Lei Zhang Tee-Kheang Ng Tee-Kheang Ng Jee Loon Foo Jee Loon Foo Hua Ling Hua Ling Won Jae Choi Won Jae Choi Won Jae Choi Matthew Wook Chang Matthew Wook Chang |
| author_facet | Gazi Sakir Hossain Gazi Sakir Hossain Saravanan Prabhu Nadarajan Saravanan Prabhu Nadarajan Lei Zhang Lei Zhang Tee-Kheang Ng Tee-Kheang Ng Jee Loon Foo Jee Loon Foo Hua Ling Hua Ling Won Jae Choi Won Jae Choi Won Jae Choi Matthew Wook Chang Matthew Wook Chang |
| author_sort | Gazi Sakir Hossain |
| collection | DOAJ |
| description | Living organisms have evolved over millions of years to fine tune their metabolism to create efficient pathways for producing metabolites necessary for their survival. Advancement in the field of synthetic biology has enabled the exploitation of these metabolic pathways for the production of desired compounds by creating microbial cell factories through metabolic engineering, thus providing sustainable routes to obtain value-added chemicals. Following the past success in metabolic engineering, there is increasing interest in diversifying natural metabolic pathways to construct non-natural biosynthesis routes, thereby creating possibilities for producing novel valuable compounds that are non-natural or without elucidated biosynthesis pathways. Thus, the range of chemicals that can be produced by biological systems can be expanded to meet the demands of industries for compounds such as plastic precursors and new antibiotics, most of which can only be obtained through chemical synthesis currently. Herein, we review and discuss novel strategies that have been developed to rewrite natural metabolic blueprints in a bid to broaden the chemical repertoire achievable in microorganisms. This review aims to provide insights on recent approaches taken to open new avenues for achieving biochemical production that are beyond currently available inventions. |
| first_indexed | 2024-12-19T04:17:27Z |
| format | Article |
| id | doaj.art-39540e8587734a76a9226f9613d0f5b6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T04:17:27Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-39540e8587734a76a9226f9613d0f5b62022-12-21T20:36:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-02-01910.3389/fmicb.2018.00155322408Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in MicroorganismsGazi Sakir Hossain0Gazi Sakir Hossain1Saravanan Prabhu Nadarajan2Saravanan Prabhu Nadarajan3Lei Zhang4Lei Zhang5Tee-Kheang Ng6Tee-Kheang Ng7Jee Loon Foo8Jee Loon Foo9Hua Ling10Hua Ling11Won Jae Choi12Won Jae Choi13Won Jae Choi14Matthew Wook Chang15Matthew Wook Chang16Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, SingaporeAgency for Science, Technology and Research (A∗STAR), Institute of Chemical and Engineering Sciences, Singapore, SingaporeDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, Singapore, SingaporeLiving organisms have evolved over millions of years to fine tune their metabolism to create efficient pathways for producing metabolites necessary for their survival. Advancement in the field of synthetic biology has enabled the exploitation of these metabolic pathways for the production of desired compounds by creating microbial cell factories through metabolic engineering, thus providing sustainable routes to obtain value-added chemicals. Following the past success in metabolic engineering, there is increasing interest in diversifying natural metabolic pathways to construct non-natural biosynthesis routes, thereby creating possibilities for producing novel valuable compounds that are non-natural or without elucidated biosynthesis pathways. Thus, the range of chemicals that can be produced by biological systems can be expanded to meet the demands of industries for compounds such as plastic precursors and new antibiotics, most of which can only be obtained through chemical synthesis currently. Herein, we review and discuss novel strategies that have been developed to rewrite natural metabolic blueprints in a bid to broaden the chemical repertoire achievable in microorganisms. This review aims to provide insights on recent approaches taken to open new avenues for achieving biochemical production that are beyond currently available inventions.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00155/fullmetabolic engineeringsynthetic biologypathway engineeringprotein engineeringbiochemical production |
| spellingShingle | Gazi Sakir Hossain Gazi Sakir Hossain Saravanan Prabhu Nadarajan Saravanan Prabhu Nadarajan Lei Zhang Lei Zhang Tee-Kheang Ng Tee-Kheang Ng Jee Loon Foo Jee Loon Foo Hua Ling Hua Ling Won Jae Choi Won Jae Choi Won Jae Choi Matthew Wook Chang Matthew Wook Chang Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms Frontiers in Microbiology metabolic engineering synthetic biology pathway engineering protein engineering biochemical production |
| title | Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms |
| title_full | Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms |
| title_fullStr | Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms |
| title_full_unstemmed | Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms |
| title_short | Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms |
| title_sort | rewriting the metabolic blueprint advances in pathway diversification in microorganisms |
| topic | metabolic engineering synthetic biology pathway engineering protein engineering biochemical production |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00155/full |
| work_keys_str_mv | AT gazisakirhossain rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT gazisakirhossain rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT saravananprabhunadarajan rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT saravananprabhunadarajan rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT leizhang rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT leizhang rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT teekheangng rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT teekheangng rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT jeeloonfoo rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT jeeloonfoo rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT hualing rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT hualing rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT wonjaechoi rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT wonjaechoi rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT wonjaechoi rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT matthewwookchang rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms AT matthewwookchang rewritingthemetabolicblueprintadvancesinpathwaydiversificationinmicroorganisms |