The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes
Autophagy is an evolutionarily conserved process of cellular self-eating which emerged these last years as a major adaptive metabolic response to various stresses such as fasting, hypoxia, or environmental pollutants. However, surprisingly very few data is currently available on its role in fish spe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-03-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.00263/full |
| _version_ | 1818191167330713600 |
|---|---|
| author | Sarah Séité Sarah Séité Sarah Séité Tracy Pioche Nicolas Ory Elisabeth Plagnes-Juan Stéphane Panserat Iban Seiliez |
| author_facet | Sarah Séité Sarah Séité Sarah Séité Tracy Pioche Nicolas Ory Elisabeth Plagnes-Juan Stéphane Panserat Iban Seiliez |
| author_sort | Sarah Séité |
| collection | DOAJ |
| description | Autophagy is an evolutionarily conserved process of cellular self-eating which emerged these last years as a major adaptive metabolic response to various stresses such as fasting, hypoxia, or environmental pollutants. However, surprisingly very few data is currently available on its role in fish species which are directly exposed to frequent environmental perturbations. Here, we report that the treatment of fasted trout hepatocytes with the autophagy inhibitor Bafilomycine A1 lowered the mRNA levels of many of the gluconeogenesis-related genes and increased those of genes involved in intracellular lipid stores. Concurrently, intracellular free amino acid levels dropped and the expression of the main genes involved in the endoplasmic reticulum (ER) stress exhibited a sharp increase in autophagy inhibited cells. Together these results highlight the strong complexity of the crosstalk between ER, autophagy and metabolism and support the importance of considering this function in future studies on metabolic adaptation of fish to environmental stresses. |
| first_indexed | 2024-12-12T00:10:18Z |
| format | Article |
| id | doaj.art-3955c70e9b384f3ea57f6efb48db79f3 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-12T00:10:18Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-3955c70e9b384f3ea57f6efb48db79f32022-12-22T00:45:00ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-03-011010.3389/fphys.2019.00263443686The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout HepatocytesSarah Séité0Sarah Séité1Sarah Séité2Tracy Pioche3Nicolas Ory4Elisabeth Plagnes-Juan5Stéphane Panserat6Iban Seiliez7INRA, E2S UPPA, UMR 1419, Nutrition, Métabolisme, Aquaculture, University of Pau and Pays de l’Adour, Saint-Pée-sur-Nivelle, FranceEvonik Rexim, Ham, FranceEvonik Nutrition and Care GmbH, Hanau, GermanyINRA, E2S UPPA, UMR 1419, Nutrition, Métabolisme, Aquaculture, University of Pau and Pays de l’Adour, Saint-Pée-sur-Nivelle, FranceINRA, E2S UPPA, UMR 1419, Nutrition, Métabolisme, Aquaculture, University of Pau and Pays de l’Adour, Saint-Pée-sur-Nivelle, FranceINRA, E2S UPPA, UMR 1419, Nutrition, Métabolisme, Aquaculture, University of Pau and Pays de l’Adour, Saint-Pée-sur-Nivelle, FranceINRA, E2S UPPA, UMR 1419, Nutrition, Métabolisme, Aquaculture, University of Pau and Pays de l’Adour, Saint-Pée-sur-Nivelle, FranceINRA, E2S UPPA, UMR 1419, Nutrition, Métabolisme, Aquaculture, University of Pau and Pays de l’Adour, Saint-Pée-sur-Nivelle, FranceAutophagy is an evolutionarily conserved process of cellular self-eating which emerged these last years as a major adaptive metabolic response to various stresses such as fasting, hypoxia, or environmental pollutants. However, surprisingly very few data is currently available on its role in fish species which are directly exposed to frequent environmental perturbations. Here, we report that the treatment of fasted trout hepatocytes with the autophagy inhibitor Bafilomycine A1 lowered the mRNA levels of many of the gluconeogenesis-related genes and increased those of genes involved in intracellular lipid stores. Concurrently, intracellular free amino acid levels dropped and the expression of the main genes involved in the endoplasmic reticulum (ER) stress exhibited a sharp increase in autophagy inhibited cells. Together these results highlight the strong complexity of the crosstalk between ER, autophagy and metabolism and support the importance of considering this function in future studies on metabolic adaptation of fish to environmental stresses.https://www.frontiersin.org/article/10.3389/fphys.2019.00263/fullfishhepatocyteautophagyintermediary metabolismER stressgene expression |
| spellingShingle | Sarah Séité Sarah Séité Sarah Séité Tracy Pioche Nicolas Ory Elisabeth Plagnes-Juan Stéphane Panserat Iban Seiliez The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes Frontiers in Physiology fish hepatocyte autophagy intermediary metabolism ER stress gene expression |
| title | The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes |
| title_full | The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes |
| title_fullStr | The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes |
| title_full_unstemmed | The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes |
| title_short | The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes |
| title_sort | autophagic flux inhibitor bafilomycine a1 affects the expression of intermediary metabolism related genes in trout hepatocytes |
| topic | fish hepatocyte autophagy intermediary metabolism ER stress gene expression |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.00263/full |
| work_keys_str_mv | AT sarahseite theautophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT sarahseite theautophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT sarahseite theautophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT tracypioche theautophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT nicolasory theautophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT elisabethplagnesjuan theautophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT stephanepanserat theautophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT ibanseiliez theautophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT sarahseite autophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT sarahseite autophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT sarahseite autophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT tracypioche autophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT nicolasory autophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT elisabethplagnesjuan autophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT stephanepanserat autophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes AT ibanseiliez autophagicfluxinhibitorbafilomycinea1affectstheexpressionofintermediarymetabolismrelatedgenesintrouthepatocytes |