Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women
<em>Chlamydia trachomatis</em> is the most common cause of sexually transmitted infection. In 75% of women and 50% of men infection is asymptomatic. According to World Health Organization reports, the number of new genital infections with <em>Chlamydia trachomatis</em> reache...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-03-01
|
| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | http://www.pagepress.org/journals/index.php/mr/article/view/3894 |
| _version_ | 1819276188269412352 |
|---|---|
| author | Parvin Hassanzadeh Hosein Sharifi Abdollah Bazargani Reza Khashei Amir Emami Mohammad Motamedifar |
| author_facet | Parvin Hassanzadeh Hosein Sharifi Abdollah Bazargani Reza Khashei Amir Emami Mohammad Motamedifar |
| author_sort | Parvin Hassanzadeh |
| collection | DOAJ |
| description | <em>Chlamydia trachomatis</em> is the most common cause of sexually transmitted infection. In 75% of women and 50% of men infection is asymptomatic. According to World Health Organization reports, the number of new genital infections with <em>Chlamydia trachomatis</em> reaches 100 million annually. The sensitivity and specificity of nacid amplification tests are 95% and 99%, respectively. Urine samples can provide a non-invasive method of testing for the detection of <em>Chlamydia trachomatis</em> by polymerase chain reaction (PCR). The aim of this study was to evaluate the prevalence of <em>Chlamydia trachomatis</em> infection in pregnant women referred to a teaching hospital affiliated to Shiraz University of Medical Sciences. Urine samples were obtained from 210 pregnant women and investigated microscopically and macroscopically by urinalysis. Precipitants were also used for DNA extraction and PCR test for detecting <em>Chlamydia trachomatis.</em> Among 210 urine specimens from women aged 15-39 years, none were positive for <em>Chlamydia trachomatis </em>by PCR. In spite of the high sensitivity and specificity of PCR, and the elimination of inhibitory effects on PCR test, no pregnant woman was positive for <em>Chlamydia trachomatis</em>. Here, we suggest that a larger sample should be studied and other sensitive methods could also be used in the future. |
| first_indexed | 2024-12-23T23:36:15Z |
| format | Article |
| id | doaj.art-3957ca82fc9d4022865ca96e7a930135 |
| institution | Directory Open Access Journal |
| issn | 2036-7473 2036-7481 |
| language | English |
| last_indexed | 2024-12-23T23:36:15Z |
| publishDate | 2012-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-3957ca82fc9d4022865ca96e7a9301352022-12-21T17:25:51ZengMDPI AGMicrobiology Research2036-74732036-74812012-03-0131e8e810.4081/mr.2012.e82076Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian womenParvin Hassanzadeh0Hosein Sharifi1Abdollah Bazargani2Reza Khashei3Amir Emami4Mohammad Motamedifar5Department of Biology, School of Science, Shiraz University, ShirazDepartment of Bacteriology and Virology, Medical School, ShirazDepartment of Bacteriology and Virology, Medical School, ShirazDepartment of Bacteriology and Virology, Medical School, ShirazDepartment of Bacteriology and Virology, Medical School, ShirazDepartment of Bacteriology and Virology, Medical School; Shiraz HIV/Aids Research Center (SHARC), Shiraz University of Medical Sciences, Shiraz<em>Chlamydia trachomatis</em> is the most common cause of sexually transmitted infection. In 75% of women and 50% of men infection is asymptomatic. According to World Health Organization reports, the number of new genital infections with <em>Chlamydia trachomatis</em> reaches 100 million annually. The sensitivity and specificity of nacid amplification tests are 95% and 99%, respectively. Urine samples can provide a non-invasive method of testing for the detection of <em>Chlamydia trachomatis</em> by polymerase chain reaction (PCR). The aim of this study was to evaluate the prevalence of <em>Chlamydia trachomatis</em> infection in pregnant women referred to a teaching hospital affiliated to Shiraz University of Medical Sciences. Urine samples were obtained from 210 pregnant women and investigated microscopically and macroscopically by urinalysis. Precipitants were also used for DNA extraction and PCR test for detecting <em>Chlamydia trachomatis.</em> Among 210 urine specimens from women aged 15-39 years, none were positive for <em>Chlamydia trachomatis </em>by PCR. In spite of the high sensitivity and specificity of PCR, and the elimination of inhibitory effects on PCR test, no pregnant woman was positive for <em>Chlamydia trachomatis</em>. Here, we suggest that a larger sample should be studied and other sensitive methods could also be used in the future.http://www.pagepress.org/journals/index.php/mr/article/view/3894polymerase chain reaction, Chlamydia trachomatis, Iran, pregnancy |
| spellingShingle | Parvin Hassanzadeh Hosein Sharifi Abdollah Bazargani Reza Khashei Amir Emami Mohammad Motamedifar Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women Microbiology Research polymerase chain reaction, Chlamydia trachomatis, Iran, pregnancy |
| title | Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women |
| title_full | Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women |
| title_fullStr | Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women |
| title_full_unstemmed | Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women |
| title_short | Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women |
| title_sort | non detection of chlamydia trachomatis infection by polymerase chain reaction in pregnant iranian women |
| topic | polymerase chain reaction, Chlamydia trachomatis, Iran, pregnancy |
| url | http://www.pagepress.org/journals/index.php/mr/article/view/3894 |
| work_keys_str_mv | AT parvinhassanzadeh nondetectionofchlamydiatrachomatisinfectionbypolymerasechainreactioninpregnantiranianwomen AT hoseinsharifi nondetectionofchlamydiatrachomatisinfectionbypolymerasechainreactioninpregnantiranianwomen AT abdollahbazargani nondetectionofchlamydiatrachomatisinfectionbypolymerasechainreactioninpregnantiranianwomen AT rezakhashei nondetectionofchlamydiatrachomatisinfectionbypolymerasechainreactioninpregnantiranianwomen AT amiremami nondetectionofchlamydiatrachomatisinfectionbypolymerasechainreactioninpregnantiranianwomen AT mohammadmotamedifar nondetectionofchlamydiatrachomatisinfectionbypolymerasechainreactioninpregnantiranianwomen |