Isolation, characterization, and detection of antibacterial activity of a bioactive compound produced by marine Bacillus sp. MH20 from Suez Bay, Egypt
This study focused on isolating and identifying a new bacterial strain from marine water and sediments of Suez bay, Timsah Lake, Egypt; capable of producing a bioactive secondary metabolite. Of the 552 bacterial isolates recovered, only 40 of these isolates exhibited antagonistic activities agains...
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| Format: | Article |
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The National Information and Documentation Centre (NIDOC) , Egypt
2019-02-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_28112_a3413924bb5247e53fcd5fffeee371f5.pdf |
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| author | Ehab Aly Beltagy Maha Abd El-Fattah Khali Mohamed Ahmed El-Shenawy Wagih Abd El-Fattah El-Shouny |
| author_facet | Ehab Aly Beltagy Maha Abd El-Fattah Khali Mohamed Ahmed El-Shenawy Wagih Abd El-Fattah El-Shouny |
| author_sort | Ehab Aly Beltagy |
| collection | DOAJ |
| description | This study focused on isolating and identifying a new bacterial strain from marine water and sediments of Suez
bay, Timsah Lake, Egypt; capable of producing a bioactive secondary metabolite. Of the 552 bacterial isolates
recovered, only 40 of these isolates exhibited antagonistic activities against different bacterial pathogens. A
promising bacterium isolated from Suez bay marine water was identified as Bacillus sp. MH20 using 16S rRNA
sequencing, and deposited in GenBank with accession number KM374670. This isolate showed sequence similarity
of 88% to B. sonorensis strain NBRC 101234. Well-cut diffusion assay was adopted to determine the in vitro
antibacterial activity of the active compound produced by this bacterium; whereas, GC-MS analysis was used to
identify the components of this bioactive substance. GC-MS analysis showed that the active principle might be
Phthalic acid, octyl 2-pentyl ester with retention time of 23.13 min. Crude extract of Bacillus sp. MK20 at a
concentration of 200 mg/l exhibited LC50 of 2.5 ppm against Artemia salina. The aims of the current work were; to
isolate and identify one of the promising bacterial strain from marine water, capable of producing bioactive
antibacterial compound and evaluating its bio-toxicity, for its potential use in the control of bacterial human, animal
and fish diseases. |
| first_indexed | 2024-04-24T08:28:52Z |
| format | Article |
| id | doaj.art-395994eeb25348fbbb113538383ad185 |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:28:52Z |
| publishDate | 2019-02-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-395994eeb25348fbbb113538383ad1852024-04-16T20:54:48ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942019-02-013125827010.21608/nrmj.2019.28112Isolation, characterization, and detection of antibacterial activity of a bioactive compound produced by marine Bacillus sp. MH20 from Suez Bay, Egypt Ehab Aly Beltagy0Maha Abd El-Fattah Khali1Mohamed Ahmed El-Shenawy2Wagih Abd El-Fattah El-Shouny3Microbiology Department, National Institute of Oceanography and Fisheries, Alexandria, EgyptBotany Department, Faculty of Science, Tanta University, Tanta, EgyptMicrobiology Department, National Institute of Oceanography and Fisheries, Alexandria, EgyptBotany Department, Faculty of Science, Tanta University, Tanta, EgyptThis study focused on isolating and identifying a new bacterial strain from marine water and sediments of Suez bay, Timsah Lake, Egypt; capable of producing a bioactive secondary metabolite. Of the 552 bacterial isolates recovered, only 40 of these isolates exhibited antagonistic activities against different bacterial pathogens. A promising bacterium isolated from Suez bay marine water was identified as Bacillus sp. MH20 using 16S rRNA sequencing, and deposited in GenBank with accession number KM374670. This isolate showed sequence similarity of 88% to B. sonorensis strain NBRC 101234. Well-cut diffusion assay was adopted to determine the in vitro antibacterial activity of the active compound produced by this bacterium; whereas, GC-MS analysis was used to identify the components of this bioactive substance. GC-MS analysis showed that the active principle might be Phthalic acid, octyl 2-pentyl ester with retention time of 23.13 min. Crude extract of Bacillus sp. MK20 at a concentration of 200 mg/l exhibited LC50 of 2.5 ppm against Artemia salina. The aims of the current work were; to isolate and identify one of the promising bacterial strain from marine water, capable of producing bioactive antibacterial compound and evaluating its bio-toxicity, for its potential use in the control of bacterial human, animal and fish diseases.https://nrmj.journals.ekb.eg/article_28112_a3413924bb5247e53fcd5fffeee371f5.pdfbacillus sp. mh20gc-ms characterizationbio-toxicityextracellular metabolite |
| spellingShingle | Ehab Aly Beltagy Maha Abd El-Fattah Khali Mohamed Ahmed El-Shenawy Wagih Abd El-Fattah El-Shouny Isolation, characterization, and detection of antibacterial activity of a bioactive compound produced by marine Bacillus sp. MH20 from Suez Bay, Egypt Novel Research in Microbiology Journal bacillus sp. mh20 gc-ms characterization bio-toxicity extracellular metabolite |
| title | Isolation, characterization, and detection of antibacterial activity of a bioactive compound produced by marine Bacillus sp. MH20 from Suez Bay, Egypt |
| title_full | Isolation, characterization, and detection of antibacterial activity of a bioactive compound produced by marine Bacillus sp. MH20 from Suez Bay, Egypt |
| title_fullStr | Isolation, characterization, and detection of antibacterial activity of a bioactive compound produced by marine Bacillus sp. MH20 from Suez Bay, Egypt |
| title_full_unstemmed | Isolation, characterization, and detection of antibacterial activity of a bioactive compound produced by marine Bacillus sp. MH20 from Suez Bay, Egypt |
| title_short | Isolation, characterization, and detection of antibacterial activity of a bioactive compound produced by marine Bacillus sp. MH20 from Suez Bay, Egypt |
| title_sort | isolation characterization and detection of antibacterial activity of a bioactive compound produced by marine bacillus sp mh20 from suez bay egypt |
| topic | bacillus sp. mh20 gc-ms characterization bio-toxicity extracellular metabolite |
| url | https://nrmj.journals.ekb.eg/article_28112_a3413924bb5247e53fcd5fffeee371f5.pdf |
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