Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study
We investigated the size-based isolation and enumeration of circulating tumor cells (CTCs) using a centrifugal microfluidic device equipped with a fluid-assisted separation technology (FAST) disc. We further assessed the correlations among CTCs, cancer antigen-125 (CA125) levels, and clinical course...
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MDPI AG
2020-04-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/4/249 |
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| author | Hyera Kim Minji Lim Jin Young Kim So-Jin Shin Yoon-Kyoung Cho Chi Heum Cho |
| author_facet | Hyera Kim Minji Lim Jin Young Kim So-Jin Shin Yoon-Kyoung Cho Chi Heum Cho |
| author_sort | Hyera Kim |
| collection | DOAJ |
| description | We investigated the size-based isolation and enumeration of circulating tumor cells (CTCs) using a centrifugal microfluidic device equipped with a fluid-assisted separation technology (FAST) disc. We further assessed the correlations among CTCs, cancer antigen-125 (CA125) levels, and clinical course of the disease in a prospective analysis of 47 serial blood samples collected at multiple time-points from 13 ovarian cancer patients. CTCs were isolated from whole blood using the FAST disc and were classified as epithelial cell adhesion molecule (EpCAM)/cytokeratin+, CD45−, and 4′,6-diamidino-2-phenylindole (DAPI)+. Mean CTC count at baseline was 20.2; 84.62% of patients had more than one CTC at baseline and had decreased CTCs counts after surgery and chemotherapy. The CTC counts in eight patients with complete responses were <3. CTC counts were correlated with CA125 levels in three patients without recurrence; they were elevated in three patients with recurrence and normal CA125 concentrations. CTC counts and CA125 levels showed high concordance with directional changes (increasing 71.4%; non-increasing 75.0%). CTC counts showed higher associations with clinical status, sensitivity (100.0% vs. 60.0%), positive predictive value (55.6% vs. 42.9%), and negative predictive value (100.0% vs. 87.5%) than CA125 levels. CTC counts were better associated with treatment response and recurrence than CA125 levels. |
| first_indexed | 2024-03-10T20:16:12Z |
| format | Article |
| id | doaj.art-395e9c0d55ae4a38b7f15f764fe2f94b |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T20:16:12Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-395e9c0d55ae4a38b7f15f764fe2f94b2023-11-19T22:31:40ZengMDPI AGDiagnostics2075-44182020-04-0110424910.3390/diagnostics10040249Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot StudyHyera Kim0Minji Lim1Jin Young Kim2So-Jin Shin3Yoon-Kyoung Cho4Chi Heum Cho5Division of Hematology/Oncology, Department of Internal Medicine, Keimyung University Dongsan Hospital, Daegu 42601, KoreaCenter for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, KoreaDivision of Hematology/Oncology, Department of Internal Medicine, Keimyung University Dongsan Hospital, Daegu 42601, KoreaDepartment of Obstetrics and Gynecology, Keimyung University Dongsan Hospital, Daegu 42601, KoreaCenter for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, KoreaDepartment of Obstetrics and Gynecology, Keimyung University Dongsan Hospital, Daegu 42601, KoreaWe investigated the size-based isolation and enumeration of circulating tumor cells (CTCs) using a centrifugal microfluidic device equipped with a fluid-assisted separation technology (FAST) disc. We further assessed the correlations among CTCs, cancer antigen-125 (CA125) levels, and clinical course of the disease in a prospective analysis of 47 serial blood samples collected at multiple time-points from 13 ovarian cancer patients. CTCs were isolated from whole blood using the FAST disc and were classified as epithelial cell adhesion molecule (EpCAM)/cytokeratin+, CD45−, and 4′,6-diamidino-2-phenylindole (DAPI)+. Mean CTC count at baseline was 20.2; 84.62% of patients had more than one CTC at baseline and had decreased CTCs counts after surgery and chemotherapy. The CTC counts in eight patients with complete responses were <3. CTC counts were correlated with CA125 levels in three patients without recurrence; they were elevated in three patients with recurrence and normal CA125 concentrations. CTC counts and CA125 levels showed high concordance with directional changes (increasing 71.4%; non-increasing 75.0%). CTC counts showed higher associations with clinical status, sensitivity (100.0% vs. 60.0%), positive predictive value (55.6% vs. 42.9%), and negative predictive value (100.0% vs. 87.5%) than CA125 levels. CTC counts were better associated with treatment response and recurrence than CA125 levels.https://www.mdpi.com/2075-4418/10/4/249circulating tumor cellsfluid-assisted separation technologycentrifugal microfluidic deviceCA125ovarian cancer |
| spellingShingle | Hyera Kim Minji Lim Jin Young Kim So-Jin Shin Yoon-Kyoung Cho Chi Heum Cho Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study Diagnostics circulating tumor cells fluid-assisted separation technology centrifugal microfluidic device CA125 ovarian cancer |
| title | Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study |
| title_full | Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study |
| title_fullStr | Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study |
| title_full_unstemmed | Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study |
| title_short | Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study |
| title_sort | circulating tumor cells enumerated by a centrifugal microfluidic device as a predictive marker for monitoring ovarian cancer treatment a pilot study |
| topic | circulating tumor cells fluid-assisted separation technology centrifugal microfluidic device CA125 ovarian cancer |
| url | https://www.mdpi.com/2075-4418/10/4/249 |
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