Intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stress
Context White tea [Camellia sinensis (L) O.Ktze. (Theaceae)] is popular in Asia, but its benefits on olfactory injury are unknown. Objective The present study explores the effects of white tea on the olfactory injury caused by chronic unpredictable mild stress (CUMS). Materials and methods C57BL/6J...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2020.1855213 |
| _version_ | 1819146385703829504 |
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| author | Wenhao Hu Guixiang Xie Tian Zhou Jialu Tu Jiayi Zhang Zejie Lin Haiyang Zhang Liangcai Gao |
| author_facet | Wenhao Hu Guixiang Xie Tian Zhou Jialu Tu Jiayi Zhang Zejie Lin Haiyang Zhang Liangcai Gao |
| author_sort | Wenhao Hu |
| collection | DOAJ |
| description | Context White tea [Camellia sinensis (L) O.Ktze. (Theaceae)] is popular in Asia, but its benefits on olfactory injury are unknown. Objective The present study explores the effects of white tea on the olfactory injury caused by chronic unpredictable mild stress (CUMS). Materials and methods C57BL/6J mice (WT) were exposed to CUMS. CUMS mice (CU) were intranasally treated with white tea extract [low tea (LT), 20 mg/kg; high tea (HT), 40 mg/kg] and fluoxetine (CF, 20 mg/kg) for 7 days. Several behavioural tests were conducted to assess depression and olfactory function. The transmission electron microscope (TEM) and semi-quantitative reverse transcription PCR were performed separately to observe the changes of related structures and genes transcription level. Results The depressive behaviours of the LT and HT mice were reversed. The latency time of the buried food pellet test decreased from 280 s (CU) to 130 s (HT), while the olfactory sensitivity and olfactory avoidance test showed that the olfactory behaviours disorder of LT and HT mice were alleviated. The white tea increased the A490 nm values of the cortisol treated cells from 0.15 to 1.4. Reduced mitochondrial and synaptic damage in the olfactory bulb (OB), enhanced expression of the brain-derived neurotrophic factor (BDNF) and olfactory marker protein (OMP) were observed in the LT and HT mice. Conclusions and discussion White tea has the potential in curing the olfactory deficiency related to chronic stress. It lays the foundation for the development of new and reliable drug to improve olfactory. |
| first_indexed | 2024-12-22T13:13:05Z |
| format | Article |
| id | doaj.art-395fdb50ed4244aa8ae04ac89f4c97e5 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-22T13:13:05Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-395fdb50ed4244aa8ae04ac89f4c97e52022-12-21T18:24:42ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162020-01-015811230123710.1080/13880209.2020.18552131855213Intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stressWenhao Hu0Guixiang Xie1Tian Zhou2Jialu Tu3Jiayi Zhang4Zejie Lin5Haiyang Zhang6Liangcai Gao7School of Life Science, East China Normal UniversitySchool of Life Science, East China Normal UniversitySchool of Life Science, East China Normal UniversityShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal UniversitySchool of Life Science, East China Normal UniversitySchool of Life Science, East China Normal UniversityShanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal UniversitySchool of Life Science, East China Normal UniversityContext White tea [Camellia sinensis (L) O.Ktze. (Theaceae)] is popular in Asia, but its benefits on olfactory injury are unknown. Objective The present study explores the effects of white tea on the olfactory injury caused by chronic unpredictable mild stress (CUMS). Materials and methods C57BL/6J mice (WT) were exposed to CUMS. CUMS mice (CU) were intranasally treated with white tea extract [low tea (LT), 20 mg/kg; high tea (HT), 40 mg/kg] and fluoxetine (CF, 20 mg/kg) for 7 days. Several behavioural tests were conducted to assess depression and olfactory function. The transmission electron microscope (TEM) and semi-quantitative reverse transcription PCR were performed separately to observe the changes of related structures and genes transcription level. Results The depressive behaviours of the LT and HT mice were reversed. The latency time of the buried food pellet test decreased from 280 s (CU) to 130 s (HT), while the olfactory sensitivity and olfactory avoidance test showed that the olfactory behaviours disorder of LT and HT mice were alleviated. The white tea increased the A490 nm values of the cortisol treated cells from 0.15 to 1.4. Reduced mitochondrial and synaptic damage in the olfactory bulb (OB), enhanced expression of the brain-derived neurotrophic factor (BDNF) and olfactory marker protein (OMP) were observed in the LT and HT mice. Conclusions and discussion White tea has the potential in curing the olfactory deficiency related to chronic stress. It lays the foundation for the development of new and reliable drug to improve olfactory.http://dx.doi.org/10.1080/13880209.2020.1855213mitochondriabehavioural experimentompbdnf |
| spellingShingle | Wenhao Hu Guixiang Xie Tian Zhou Jialu Tu Jiayi Zhang Zejie Lin Haiyang Zhang Liangcai Gao Intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stress Pharmaceutical Biology mitochondria behavioural experiment omp bdnf |
| title | Intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stress |
| title_full | Intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stress |
| title_fullStr | Intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stress |
| title_full_unstemmed | Intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stress |
| title_short | Intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stress |
| title_sort | intranasal administration of white tea alleviates the olfactory function deficit induced by chronic unpredictable mild stress |
| topic | mitochondria behavioural experiment omp bdnf |
| url | http://dx.doi.org/10.1080/13880209.2020.1855213 |
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