Blood Glukose Response of Giant Gouramy (Osphronemus gaouramy, Lac.) to the Stress of Environmental Temperature Changes
<p>This experiment was conducted to investigate blood glucose performance of giant gouramy <em>(Osphronemus gouramy, </em>Lac.) to environmental changes. Fish with body weight of about 52,15 g was used in the experiment. A hundred and twenty fish were subjected to stress by moving...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Asosiasi Sains Akuakultur Indonesia
2007-08-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | http://journal.ipb.ac.id/index.php/jai/article/view/3878 |
| Summary: | <p>This experiment was conducted to investigate blood glucose performance of giant gouramy <em>(Osphronemus gouramy, </em>Lac.) to environmental changes. Fish with body weight of about 52,15 g was used in the experiment. A hundred and twenty fish were subjected to stress by moving them to another aquarium containing cooler water for 5 minute before put them back to the origin aquarium. The stress treatments were Δ 0°C (A), Δ-3°C (B), Δ-6°C(C), and Δ-9°C(D). Blood glucose was measured at 0, 1, 2, 3, 4 and 5 hours post stress, each for 5 fish. During stress treatment, the survival offish were recorded. To study the role of insulin activation on reducing the stress effects, thirty fish were injected with insulin 2 IU/100 g body weight before subjected them to stressar. Blood glucose level of fish subjected to temperature stress of Δ-9°C was the greatest. The blood glucose response to temperature changes was linear, Y = 4,4543 X + 35,553 with R2 = 0,09976. The survival rate of fish was 100% for all treatments. Injected of insulin 2 IU/100 g body weight was able to reduce hyperglycemia that caused by stress.</p> <p>Key words: Blood glucose, giant gouramy, <em>Osphronemus gouramy, </em>stress</p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan dengan tujuan untuk mengetahui performa glukosa darah ikan gurami <em>(Osphronemus gouramy, </em>Lac.) dalam merespon perubahan suhu lingkungan. Ikan berbobot rata-rata 52,15 g sebanyak 120 ekor diberi stres dengan cara diangkat dan dipindahkan ke suatu wadah yang bersuhu lebih dingin selama 5 menit dan dikembalikan lagi ke wadah mula-mula. Perlakuan stres perubahan suhu dingin tersebut adalah A (Δ 0°C), B (Δ- 3°C), C (Δ-6°C) dan D (Δ-9°C). Glukosa darah diukur dari 5 ekor ikan pada jam ke 0, 1, 2, 3, 4 dan 5 jam pascastres. Kelangsungan hidup dihitung pada saat perlakuan stres. Untuk melihat peran aktivasi insulin dalam menekan efek stres, ikan sebanyak 30 ekor diinjeksi insulin 2 iu/100 g bobot badan sebelum diberi stres. Kadar glukosa darah ikan gurame yang diberi stres perubahan suhu dingin sebesar Δ-9°C mengalami peningkatan paling besar. Respon glukosa darah terhadap stres perubahan suhu tersebut berpola linier Y= 4,4543 X + 35,553, dengan R2 = 0,9976. Perlakuan tersebut menghasilkan kelangsungan hidup yang sama yaitu 100%. Injeksi insulin 2 IU/100 g bobot badan mampu menekan hiperglisemia akibat stres.</p> Kata kunci: Glukosa darah, ikan gurami, <em>Osphronemus gouramy, </em>stres |
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| ISSN: | 1412-5269 2354-6700 |