Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs
Abstract Adipose tissue is a metabolic and endocrine organ, and its adipocytes can synthesize and secrete extracellular vesicles (EVs), thus allowing intercellular communication. EVs are nanoparticles that transport lipids, proteins, metabolites, and nucleic acids (mRNA and microRNAs). MicroRNAs (mi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20523-x |
| _version_ | 1797998430087282688 |
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| author | Paola Caroline da Silva Nunes Rosane Mazzarella Juliano Coelho da Silveira Deise Carla Almeida Leite Dellova |
| author_facet | Paola Caroline da Silva Nunes Rosane Mazzarella Juliano Coelho da Silveira Deise Carla Almeida Leite Dellova |
| author_sort | Paola Caroline da Silva Nunes |
| collection | DOAJ |
| description | Abstract Adipose tissue is a metabolic and endocrine organ, and its adipocytes can synthesize and secrete extracellular vesicles (EVs), thus allowing intercellular communication. EVs are nanoparticles that transport lipids, proteins, metabolites, and nucleic acids (mRNA and microRNAs). MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression. miR-132, miR-26b, and miR-155 are associated with obesity, lipid metabolism and adipogenesis. The aim of this study was to evaluate the enriched EVs fraction containing miRNAs (miR-132, miR-26b, and miR-155) in serum from obese female dogs. Thirty-two neutered females in good general condition were recruited, including 21 obese and 11 healthy controls. The initial evaluation of the females included a general physical examination and laboratory tests. Small EVs (sEVs) were isolated from whole blood by serial centrifugation and ultracentrifugation, and nanoparticle analysis was used to determine the size and concentration of serum sEVs. miRNAs were extracted from sEVs enriched fraction and analyzed by real-time polymerase chain reaction. Obese female dogs with hypertriglyceridemia showed an increase in the sEVs concentration and in the expression of miR-132 and miR-26b in sEVs enriched fraction. No changes were observed in the group of obese female dogs with normal serum biochemical profile and in relation to miR-155 expression. These results suggest that obese female dogs with hypertriglyceridemia may present alterations in sEVs and in the expression of miRNAs related to lipid metabolism and adipogenesis. |
| first_indexed | 2024-04-11T10:48:38Z |
| format | Article |
| id | doaj.art-396cfe9325584709aa9a4a84e95fc238 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T10:48:38Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-396cfe9325584709aa9a4a84e95fc2382022-12-22T04:28:58ZengNature PortfolioScientific Reports2045-23222022-09-0112111110.1038/s41598-022-20523-xEvaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogsPaola Caroline da Silva Nunes0Rosane Mazzarella1Juliano Coelho da Silveira2Deise Carla Almeida Leite Dellova3Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São PauloDepartment of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São PauloDepartment of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São PauloDepartment of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São PauloAbstract Adipose tissue is a metabolic and endocrine organ, and its adipocytes can synthesize and secrete extracellular vesicles (EVs), thus allowing intercellular communication. EVs are nanoparticles that transport lipids, proteins, metabolites, and nucleic acids (mRNA and microRNAs). MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression. miR-132, miR-26b, and miR-155 are associated with obesity, lipid metabolism and adipogenesis. The aim of this study was to evaluate the enriched EVs fraction containing miRNAs (miR-132, miR-26b, and miR-155) in serum from obese female dogs. Thirty-two neutered females in good general condition were recruited, including 21 obese and 11 healthy controls. The initial evaluation of the females included a general physical examination and laboratory tests. Small EVs (sEVs) were isolated from whole blood by serial centrifugation and ultracentrifugation, and nanoparticle analysis was used to determine the size and concentration of serum sEVs. miRNAs were extracted from sEVs enriched fraction and analyzed by real-time polymerase chain reaction. Obese female dogs with hypertriglyceridemia showed an increase in the sEVs concentration and in the expression of miR-132 and miR-26b in sEVs enriched fraction. No changes were observed in the group of obese female dogs with normal serum biochemical profile and in relation to miR-155 expression. These results suggest that obese female dogs with hypertriglyceridemia may present alterations in sEVs and in the expression of miRNAs related to lipid metabolism and adipogenesis.https://doi.org/10.1038/s41598-022-20523-x |
| spellingShingle | Paola Caroline da Silva Nunes Rosane Mazzarella Juliano Coelho da Silveira Deise Carla Almeida Leite Dellova Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs Scientific Reports |
| title | Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs |
| title_full | Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs |
| title_fullStr | Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs |
| title_full_unstemmed | Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs |
| title_short | Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs |
| title_sort | evaluation of circulating extracellular vesicles and mirna in neutered and obese female dogs |
| url | https://doi.org/10.1038/s41598-022-20523-x |
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