Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic Carcinoma
Objective: Cathepsin D (CTSD) is a pivotal orchestrator in the occurrence and development of tumors. Recently, CTSD was detected in salivary adenoid cystic carcinoma (SACC). However, its functional role in perineural invasion (PNI) of SACC remained elusive. We conducted the present study to detect t...
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| Format: | Article |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2018.00492/full |
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| author | Mei Zhang Mei Zhang Mei Zhang Jia-shun Wu Jia-shun Wu Jia-shun Wu Xiao Yang Xiao Yang Xiao Yang Xin Pang Xin Pang Xin Pang Li Li Sha-sha Wang Sha-sha Wang Sha-sha Wang Jing-biao Wu Jing-biao Wu Jing-biao Wu Ya-jie Tang Xin-hua Liang Xin-hua Liang Xin-hua Liang Min Zheng Ya-ling Tang Ya-ling Tang Ya-ling Tang |
| author_facet | Mei Zhang Mei Zhang Mei Zhang Jia-shun Wu Jia-shun Wu Jia-shun Wu Xiao Yang Xiao Yang Xiao Yang Xin Pang Xin Pang Xin Pang Li Li Sha-sha Wang Sha-sha Wang Sha-sha Wang Jing-biao Wu Jing-biao Wu Jing-biao Wu Ya-jie Tang Xin-hua Liang Xin-hua Liang Xin-hua Liang Min Zheng Ya-ling Tang Ya-ling Tang Ya-ling Tang |
| author_sort | Mei Zhang |
| collection | DOAJ |
| description | Objective: Cathepsin D (CTSD) is a pivotal orchestrator in the occurrence and development of tumors. Recently, CTSD was detected in salivary adenoid cystic carcinoma (SACC). However, its functional role in perineural invasion (PNI) of SACC remained elusive. We conducted the present study to detect the expression of CTSD in SACC, analyze the correlation between CTSD expression and prognosis of SACC patients and elucidate the role of CTSD in occurrence of PNI in SACC to lay the foundation for further studies.Methods: Immunohistochemical analysis was conducted to assess CTSD and Ki67 expression in 158 SACC samples and 20 normal salivary gland samples adjacent to carcinoma. Meanwhile, the correlation between CTSD and PNI of SACC specimens was analyzed using Wilcoxon test. QRT-PCR, immunofluorescence and western blot analysis were used to examine the levels of CTSD mRNA and protein in SACC-LM cell line. SiRNA-mediated CTSD silence was performed. Scratch wound healing assay, transwell invasion assay and DRG co-culture assay of PNI was used to detect the ability of migration, invasion and PNI. FITC-phalloidin was used to detect cytoskeletal organization.Results: Our data demonstrated that the positive expression of CTSD was observed in 74.1% (117/158) of SACC cases, and the expression of CTSD was significantly correlated with the PNI (p < 0.05). The ability of migration, invasion, and PNI could be inhibited significantly by siRNA-mediated CTSD silence (p < 0.01). Furthermore, siRNA-mediated CTSD silence inhibited cytoskeletal organization and pseudo foot formation in SACC-LM cells.Conclusion: Our results suggested that an association between PNI and expression of CTSD existed. CTSD may promote PNI of SACC accompanied by cytoskeletal organization and pseudo foot formation. |
| first_indexed | 2024-12-12T09:19:41Z |
| format | Article |
| id | doaj.art-39719c72bea14acb8969d9adbc8f0528 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T09:19:41Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-39719c72bea14acb8969d9adbc8f05282022-12-22T00:29:15ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2018-10-01810.3389/fonc.2018.00492407725Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic CarcinomaMei Zhang0Mei Zhang1Mei Zhang2Jia-shun Wu3Jia-shun Wu4Jia-shun Wu5Xiao Yang6Xiao Yang7Xiao Yang8Xin Pang9Xin Pang10Xin Pang11Li Li12Sha-sha Wang13Sha-sha Wang14Sha-sha Wang15Jing-biao Wu16Jing-biao Wu17Jing-biao Wu18Ya-jie Tang19Xin-hua Liang20Xin-hua Liang21Xin-hua Liang22Min Zheng23Ya-ling Tang24Ya-ling Tang25Ya-ling Tang26State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaNational Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaNational Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaNational Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaNational Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Stomatology, Zhoushan Hospital, Wenzhou Medical University, Zhoushan, ChinaState Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaNational Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaNational Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaKey Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, ChinaState Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaNational Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Stomatology, Zhoushan Hospital, Wenzhou Medical University, Zhoushan, ChinaState Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaNational Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaObjective: Cathepsin D (CTSD) is a pivotal orchestrator in the occurrence and development of tumors. Recently, CTSD was detected in salivary adenoid cystic carcinoma (SACC). However, its functional role in perineural invasion (PNI) of SACC remained elusive. We conducted the present study to detect the expression of CTSD in SACC, analyze the correlation between CTSD expression and prognosis of SACC patients and elucidate the role of CTSD in occurrence of PNI in SACC to lay the foundation for further studies.Methods: Immunohistochemical analysis was conducted to assess CTSD and Ki67 expression in 158 SACC samples and 20 normal salivary gland samples adjacent to carcinoma. Meanwhile, the correlation between CTSD and PNI of SACC specimens was analyzed using Wilcoxon test. QRT-PCR, immunofluorescence and western blot analysis were used to examine the levels of CTSD mRNA and protein in SACC-LM cell line. SiRNA-mediated CTSD silence was performed. Scratch wound healing assay, transwell invasion assay and DRG co-culture assay of PNI was used to detect the ability of migration, invasion and PNI. FITC-phalloidin was used to detect cytoskeletal organization.Results: Our data demonstrated that the positive expression of CTSD was observed in 74.1% (117/158) of SACC cases, and the expression of CTSD was significantly correlated with the PNI (p < 0.05). The ability of migration, invasion, and PNI could be inhibited significantly by siRNA-mediated CTSD silence (p < 0.01). Furthermore, siRNA-mediated CTSD silence inhibited cytoskeletal organization and pseudo foot formation in SACC-LM cells.Conclusion: Our results suggested that an association between PNI and expression of CTSD existed. CTSD may promote PNI of SACC accompanied by cytoskeletal organization and pseudo foot formation.https://www.frontiersin.org/article/10.3389/fonc.2018.00492/fullcathepsin D (CTSD)salivary adenoid cystic carcinoma (SACC)perineural invasion (PNI)invasion frontiercytoskeletal organization |
| spellingShingle | Mei Zhang Mei Zhang Mei Zhang Jia-shun Wu Jia-shun Wu Jia-shun Wu Xiao Yang Xiao Yang Xiao Yang Xin Pang Xin Pang Xin Pang Li Li Sha-sha Wang Sha-sha Wang Sha-sha Wang Jing-biao Wu Jing-biao Wu Jing-biao Wu Ya-jie Tang Xin-hua Liang Xin-hua Liang Xin-hua Liang Min Zheng Ya-ling Tang Ya-ling Tang Ya-ling Tang Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic Carcinoma Frontiers in Oncology cathepsin D (CTSD) salivary adenoid cystic carcinoma (SACC) perineural invasion (PNI) invasion frontier cytoskeletal organization |
| title | Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic Carcinoma |
| title_full | Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic Carcinoma |
| title_fullStr | Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic Carcinoma |
| title_full_unstemmed | Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic Carcinoma |
| title_short | Overexpression Cathepsin D Contributes to Perineural Invasion of Salivary Adenoid Cystic Carcinoma |
| title_sort | overexpression cathepsin d contributes to perineural invasion of salivary adenoid cystic carcinoma |
| topic | cathepsin D (CTSD) salivary adenoid cystic carcinoma (SACC) perineural invasion (PNI) invasion frontier cytoskeletal organization |
| url | https://www.frontiersin.org/article/10.3389/fonc.2018.00492/full |
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