Development of TaqMan<sup>® </sup>MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1
<p>Abstract</p> <p>Background</p> <p>Anatid herpesvirus 1 (AHV-1) is an alphaherpesvirus associated with latent infection and mortality in ducks and geese and is currently affecting the world-wide waterfowl production severely. Here we describe a fluorescent quantitativ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-06-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/6/1/71 |
| _version_ | 1818667173389795328 |
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| author | Shen Chanjuan Wang Mingshu Cheng Anchun Guo Yufei Jia Renyong Chen Shun Zhang Na |
| author_facet | Shen Chanjuan Wang Mingshu Cheng Anchun Guo Yufei Jia Renyong Chen Shun Zhang Na |
| author_sort | Shen Chanjuan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Anatid herpesvirus 1 (AHV-1) is an alphaherpesvirus associated with latent infection and mortality in ducks and geese and is currently affecting the world-wide waterfowl production severely. Here we describe a fluorescent quantitative real-time PCR (FQ-PCR) method developed for fast measurement of AHV-1 DNA based on TaqMan MGB technology.</p> <p>Results</p> <p>The detection limit of the assay was 1 × 10<sup>1 </sup>standard DNA copies, with a sensitivity of 2 logs higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intra-assay and inter-assay coefficients of variation.</p> <p>Conclusion</p> <p>The high sensitivity, specificity, simplicity and reproducibility of the AHV-1 fluorogenic PCR assay, combined with its wide dynamic range and high throughput, make this method suitable for a broad spectrum of AHV-1 etiologically related application.</p> |
| first_indexed | 2024-12-17T06:16:13Z |
| format | Article |
| id | doaj.art-3974680bb2d24a93b332e7a7e44bb235 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-17T06:16:13Z |
| publishDate | 2009-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-3974680bb2d24a93b332e7a7e44bb2352022-12-21T22:00:30ZengBMCVirology Journal1743-422X2009-06-01617110.1186/1743-422X-6-71Development of TaqMan<sup>® </sup>MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1Shen ChanjuanWang MingshuCheng AnchunGuo YufeiJia RenyongChen ShunZhang Na<p>Abstract</p> <p>Background</p> <p>Anatid herpesvirus 1 (AHV-1) is an alphaherpesvirus associated with latent infection and mortality in ducks and geese and is currently affecting the world-wide waterfowl production severely. Here we describe a fluorescent quantitative real-time PCR (FQ-PCR) method developed for fast measurement of AHV-1 DNA based on TaqMan MGB technology.</p> <p>Results</p> <p>The detection limit of the assay was 1 × 10<sup>1 </sup>standard DNA copies, with a sensitivity of 2 logs higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intra-assay and inter-assay coefficients of variation.</p> <p>Conclusion</p> <p>The high sensitivity, specificity, simplicity and reproducibility of the AHV-1 fluorogenic PCR assay, combined with its wide dynamic range and high throughput, make this method suitable for a broad spectrum of AHV-1 etiologically related application.</p>http://www.virologyj.com/content/6/1/71 |
| spellingShingle | Shen Chanjuan Wang Mingshu Cheng Anchun Guo Yufei Jia Renyong Chen Shun Zhang Na Development of TaqMan<sup>® </sup>MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 Virology Journal |
| title | Development of TaqMan<sup>® </sup>MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 |
| title_full | Development of TaqMan<sup>® </sup>MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 |
| title_fullStr | Development of TaqMan<sup>® </sup>MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 |
| title_full_unstemmed | Development of TaqMan<sup>® </sup>MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 |
| title_short | Development of TaqMan<sup>® </sup>MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 |
| title_sort | development of taqman sup r sup mgb fluorescent real time pcr assay for the detection of anatid herpesvirus 1 |
| url | http://www.virologyj.com/content/6/1/71 |
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