Different wild type strains of zebrafish show divergent susceptibility to TNBS-induced intestinal inflammation displaying distinct immune cell profiles
Little is known about the diversity in immune profile of the different wild type strains of zebrafish (Danio rerio), despite its growing popularity as an animal model to study human diseases and drug testing. In the case of data resulting from modeling human diseases, differences in the background D...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Current Research in Immunology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590255521000275 |
| _version_ | 1811295640893784064 |
|---|---|
| author | Barbara Nunes Padovani Mariana Abrantes do Amaral Camila Morales Fénero Lais Cavalieri Paredes Guilherme José Boturra de Barros Izabella Karina Xavier Meire Ioshie Hiyane Bruno Ghirotto Carmen G. Feijóo Niels Olsen Saraiva Câmara Tatiana Takiishi |
| author_facet | Barbara Nunes Padovani Mariana Abrantes do Amaral Camila Morales Fénero Lais Cavalieri Paredes Guilherme José Boturra de Barros Izabella Karina Xavier Meire Ioshie Hiyane Bruno Ghirotto Carmen G. Feijóo Niels Olsen Saraiva Câmara Tatiana Takiishi |
| author_sort | Barbara Nunes Padovani |
| collection | DOAJ |
| description | Little is known about the diversity in immune profile of the different wild type strains of zebrafish (Danio rerio), despite its growing popularity as an animal model to study human diseases and drug testing. In the case of data resulting from modeling human diseases, differences in the background Danio fishes have rarely been taken into consideration when interpreting results and this is potentially problematic, as many studies not even mention the source and strain of the animals. In this study, we hypothesized that different wild type zebrafish strains could present distinct immune traits. To address the differences in immune responses between two commonly used wild type strains of zebrafish, AB and Tübingen (TU), we used an intestinal inflammation model induced by 2,4,6-Trinitrobenzenesulfonic acid (TNBS) and characterized the susceptibility and immune profile in these two strains. Our data demonstrates significant differences in survival between AB and TU strains when exposed to TNBS, suggesting important physiological differences in how these strains respond to inflammatory challenges. We observed that the AB strain presented increased mortality, higher neutrophilic intestinal infiltration, decreased goblet cell numbers and decreased IL-10 expression when exposed to TNBS, compared to the TU strain. In summary, our study demonstrates strain-specific immunological responses in AB and TU animals. Finally, the significant variations in strain-related susceptibility to inflammation and the differences in the immune profile shown here, highlight that the background of each strain need to be considered when utilizing zebrafish to model diseases and for drug screening purposes, thus better immune characterization of the diverse wild type strains of zebrafish is imperative. |
| first_indexed | 2024-04-13T05:36:27Z |
| format | Article |
| id | doaj.art-3975124da59241b591175f2bd38eefc9 |
| institution | Directory Open Access Journal |
| issn | 2590-2555 |
| language | English |
| last_indexed | 2024-04-13T05:36:27Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Immunology |
| spelling | doaj.art-3975124da59241b591175f2bd38eefc92022-12-22T03:00:16ZengElsevierCurrent Research in Immunology2590-25552022-01-0131322Different wild type strains of zebrafish show divergent susceptibility to TNBS-induced intestinal inflammation displaying distinct immune cell profilesBarbara Nunes Padovani0Mariana Abrantes do Amaral1Camila Morales Fénero2Lais Cavalieri Paredes3Guilherme José Boturra de Barros4Izabella Karina Xavier5Meire Ioshie Hiyane6Bruno Ghirotto7Carmen G. Feijóo8Niels Olsen Saraiva Câmara9Tatiana Takiishi10Institute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-000 Brazil; Corresponding author. Avenida Professor Lineu Prestes, 1730 Cidade Universitária, São Paulo, 05508-000, Brazil.Nephrology Division, Department of Medicine, Federal University of São Paulo, São Paulo, SP, BrazilInstitute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-000 BrazilInstitute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-000 BrazilInstitute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-000 BrazilInstitute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-000 BrazilInstitute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-000 BrazilInstitute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-000 BrazilFacultad Ciencias de la Vida, Universidad Andres Bello, Santiago, 837014, ChileInstitute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-000 BrazilULB Center for Diabetes Research, Université Libre de Bruxelles, Brussels, BelgiumLittle is known about the diversity in immune profile of the different wild type strains of zebrafish (Danio rerio), despite its growing popularity as an animal model to study human diseases and drug testing. In the case of data resulting from modeling human diseases, differences in the background Danio fishes have rarely been taken into consideration when interpreting results and this is potentially problematic, as many studies not even mention the source and strain of the animals. In this study, we hypothesized that different wild type zebrafish strains could present distinct immune traits. To address the differences in immune responses between two commonly used wild type strains of zebrafish, AB and Tübingen (TU), we used an intestinal inflammation model induced by 2,4,6-Trinitrobenzenesulfonic acid (TNBS) and characterized the susceptibility and immune profile in these two strains. Our data demonstrates significant differences in survival between AB and TU strains when exposed to TNBS, suggesting important physiological differences in how these strains respond to inflammatory challenges. We observed that the AB strain presented increased mortality, higher neutrophilic intestinal infiltration, decreased goblet cell numbers and decreased IL-10 expression when exposed to TNBS, compared to the TU strain. In summary, our study demonstrates strain-specific immunological responses in AB and TU animals. Finally, the significant variations in strain-related susceptibility to inflammation and the differences in the immune profile shown here, highlight that the background of each strain need to be considered when utilizing zebrafish to model diseases and for drug screening purposes, thus better immune characterization of the diverse wild type strains of zebrafish is imperative.http://www.sciencedirect.com/science/article/pii/S2590255521000275ZebrafishABTübingenIntestinal inflammationTNBS |
| spellingShingle | Barbara Nunes Padovani Mariana Abrantes do Amaral Camila Morales Fénero Lais Cavalieri Paredes Guilherme José Boturra de Barros Izabella Karina Xavier Meire Ioshie Hiyane Bruno Ghirotto Carmen G. Feijóo Niels Olsen Saraiva Câmara Tatiana Takiishi Different wild type strains of zebrafish show divergent susceptibility to TNBS-induced intestinal inflammation displaying distinct immune cell profiles Current Research in Immunology Zebrafish AB Tübingen Intestinal inflammation TNBS |
| title | Different wild type strains of zebrafish show divergent susceptibility to TNBS-induced intestinal inflammation displaying distinct immune cell profiles |
| title_full | Different wild type strains of zebrafish show divergent susceptibility to TNBS-induced intestinal inflammation displaying distinct immune cell profiles |
| title_fullStr | Different wild type strains of zebrafish show divergent susceptibility to TNBS-induced intestinal inflammation displaying distinct immune cell profiles |
| title_full_unstemmed | Different wild type strains of zebrafish show divergent susceptibility to TNBS-induced intestinal inflammation displaying distinct immune cell profiles |
| title_short | Different wild type strains of zebrafish show divergent susceptibility to TNBS-induced intestinal inflammation displaying distinct immune cell profiles |
| title_sort | different wild type strains of zebrafish show divergent susceptibility to tnbs induced intestinal inflammation displaying distinct immune cell profiles |
| topic | Zebrafish AB Tübingen Intestinal inflammation TNBS |
| url | http://www.sciencedirect.com/science/article/pii/S2590255521000275 |
| work_keys_str_mv | AT barbaranunespadovani differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT marianaabrantesdoamaral differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT camilamoralesfenero differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT laiscavalieriparedes differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT guilhermejoseboturradebarros differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT izabellakarinaxavier differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT meireioshiehiyane differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT brunoghirotto differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT carmengfeijoo differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT nielsolsensaraivacamara differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles AT tatianatakiishi differentwildtypestrainsofzebrafishshowdivergentsusceptibilitytotnbsinducedintestinalinflammationdisplayingdistinctimmunecellprofiles |