Cytoprotective activity of extracts from Adelia ricinella L. aerial parts.
Context: Several factors can affect the erythrocyte cell membrane integrity targeting important hematological alterations that can be avoided by the cytoprotective effect offered by some plant extracts. Aims: To evaluate the cytoprotective activity of Adelia ricinella L. extracts on red blood cel...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
GarVal Editorial Ltda.
2021-11-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol9/jppres21.1093_9.6.892.pdf |
| _version_ | 1818339079746486272 |
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| author | Clara A. Berenguer-Rivas Onel Fong Lores Julio C. Escalona-Arranz Jorge de la Vega-Acosta Diana J. Arro-Díaz Frenkel Guisado-Bourzac Gabriel Llauradó-Maury Humberto J. Morris-Quevedo |
| author_facet | Clara A. Berenguer-Rivas Onel Fong Lores Julio C. Escalona-Arranz Jorge de la Vega-Acosta Diana J. Arro-Díaz Frenkel Guisado-Bourzac Gabriel Llauradó-Maury Humberto J. Morris-Quevedo |
| author_sort | Clara A. Berenguer-Rivas |
| collection | DOAJ |
| description | Context: Several factors can affect the erythrocyte cell membrane integrity targeting important hematological alterations that can be avoided by the cytoprotective effect offered by some plant extracts.
Aims: To evaluate the cytoprotective activity of Adelia ricinella L. extracts on red blood cells (RBCs) membrane after hypotonic and oxidative treatments.
Methods: Total phenols and flavonoid contents were spectrophotometrically determined in three extracts: AR1 (ethanol 95%), AR2 (ethanol 50%), and AR3 (aqueous extract). Luteolin and apigenin were quantified using HPLC-DAD techniques. Hypotonic erythrocyte membrane stabilizing activity of plant extracts, their antioxidant protective effect on H2O2-induced cell damage, and cytotoxicity on Vero cells were explored. Control cells were treated with sodium diclofenac or ascorbic acid.
Results: AR2 extract showed the highest values of total phenols/flavonoids, as well as, for luteolin and apigenin with 207.5 and 1.86 µg/mL respectively. The extracts did not exert spontaneous hemolysis following the INVITOX protocol, presumably by the protective effect of high flavonoid content. A concentration-dependent pattern was observed on the hypotonic erythrocyte membrane stabilizing assay, in which both ethanol extracts but mainly AR1 (IC50 = 16.46 µg/mL) showed a significant activity with lower IC50 values than diclofenac-control group. On the other hand, AR2 (IC50 = 17.49 µg/mL) displayed the most potent cytoprotective effect on RBCs after H2O2-induced cell damage. Adelia ricinella extracts were not cytotoxic to mammalian Vero cells (IC50 > 256 μg/mL).
Conclusions: The study suggests that Adelia ricinella extracts can promote erythrocyte cytoprotection by protecting both membrane layers, thus preventing potential hematological alterations induced by oxidizing damage and probably, in inflammation-related diseases. |
| first_indexed | 2024-12-13T15:21:18Z |
| format | Article |
| id | doaj.art-3975c37b8c8346b5a89b575d4cf671ba |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-13T15:21:18Z |
| publishDate | 2021-11-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-3975c37b8c8346b5a89b575d4cf671ba2022-12-21T23:40:33ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502021-11-0196892904Cytoprotective activity of extracts from Adelia ricinella L. aerial parts.Clara A. Berenguer-Rivas0Onel Fong Lores1Julio C. Escalona-Arranz2Jorge de la Vega-Acosta3Diana J. Arro-Díaz4Frenkel Guisado-Bourzac5Gabriel Llauradó-Maury6Humberto J. Morris-Quevedo7Department of Pharmacy, Faculty of Natural and Exact Sciences, Universidad de Oriente, Santiago de Cuba 5, CP 90500, Cuba.Center of Toxicology and Biomedicine (TOXIMED), Medical University of Santiago de Cuba, Santiago de Cuba 4, CP 90400, Cuba.Department of Pharmacy, Faculty of Natural and Exact Sciences, Universidad de Oriente, Santiago de Cuba 5, CP 90500, Cuba.Center of Toxicology and Biomedicine (TOXIMED), Medical University of Santiago de Cuba, Santiago de Cuba 4, CP 90400, Cuba.Center of Toxicology and Biomedicine (TOXIMED), Medical University of Santiago de Cuba, Santiago de Cuba 4, CP 90400, Cuba.Laboratory of Genetic and Applied Genomics. School of Marine Sciences. Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.Center of Studies for Industrial Biotechnology (CEBI), Universidad de Oriente, Santiago de Cuba 5, CP 90500, Cuba.Center of Studies for Industrial Biotechnology (CEBI), Universidad de Oriente, Santiago de Cuba 5, CP 90500, Cuba.Context: Several factors can affect the erythrocyte cell membrane integrity targeting important hematological alterations that can be avoided by the cytoprotective effect offered by some plant extracts. Aims: To evaluate the cytoprotective activity of Adelia ricinella L. extracts on red blood cells (RBCs) membrane after hypotonic and oxidative treatments. Methods: Total phenols and flavonoid contents were spectrophotometrically determined in three extracts: AR1 (ethanol 95%), AR2 (ethanol 50%), and AR3 (aqueous extract). Luteolin and apigenin were quantified using HPLC-DAD techniques. Hypotonic erythrocyte membrane stabilizing activity of plant extracts, their antioxidant protective effect on H2O2-induced cell damage, and cytotoxicity on Vero cells were explored. Control cells were treated with sodium diclofenac or ascorbic acid. Results: AR2 extract showed the highest values of total phenols/flavonoids, as well as, for luteolin and apigenin with 207.5 and 1.86 µg/mL respectively. The extracts did not exert spontaneous hemolysis following the INVITOX protocol, presumably by the protective effect of high flavonoid content. A concentration-dependent pattern was observed on the hypotonic erythrocyte membrane stabilizing assay, in which both ethanol extracts but mainly AR1 (IC50 = 16.46 µg/mL) showed a significant activity with lower IC50 values than diclofenac-control group. On the other hand, AR2 (IC50 = 17.49 µg/mL) displayed the most potent cytoprotective effect on RBCs after H2O2-induced cell damage. Adelia ricinella extracts were not cytotoxic to mammalian Vero cells (IC50 > 256 μg/mL). Conclusions: The study suggests that Adelia ricinella extracts can promote erythrocyte cytoprotection by protecting both membrane layers, thus preventing potential hematological alterations induced by oxidizing damage and probably, in inflammation-related diseases.https://jppres.com/jppres/pdf/vol9/jppres21.1093_9.6.892.pdfadelia ricinellaantioxidantcytoprotectionflavonoidsred blood cells |
| spellingShingle | Clara A. Berenguer-Rivas Onel Fong Lores Julio C. Escalona-Arranz Jorge de la Vega-Acosta Diana J. Arro-Díaz Frenkel Guisado-Bourzac Gabriel Llauradó-Maury Humberto J. Morris-Quevedo Cytoprotective activity of extracts from Adelia ricinella L. aerial parts. Journal of Pharmacy & Pharmacognosy Research adelia ricinella antioxidant cytoprotection flavonoids red blood cells |
| title | Cytoprotective activity of extracts from Adelia ricinella L. aerial parts. |
| title_full | Cytoprotective activity of extracts from Adelia ricinella L. aerial parts. |
| title_fullStr | Cytoprotective activity of extracts from Adelia ricinella L. aerial parts. |
| title_full_unstemmed | Cytoprotective activity of extracts from Adelia ricinella L. aerial parts. |
| title_short | Cytoprotective activity of extracts from Adelia ricinella L. aerial parts. |
| title_sort | cytoprotective activity of extracts from adelia ricinella l aerial parts |
| topic | adelia ricinella antioxidant cytoprotection flavonoids red blood cells |
| url | https://jppres.com/jppres/pdf/vol9/jppres21.1093_9.6.892.pdf |
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