Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue Outbreak
After a dengue outbreak, the knowledge on the extent, distribution and mechanisms of insecticide resistance is essential for successful insecticide-based dengue control interventions. Therefore, we evaluated the potential changes to insecticide resistance in natural Aedes aegypti populations to Orga...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Entomologia
2016-01-01
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| Series: | Revista Brasileira de Entomologia |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0085562615001351 |
| _version_ | 1828418435036479488 |
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| author | Oscar Alexander Aguirre-Obando Anna Julia Pietrobon Ana Caroline Dalla Bona Mário Antônio Navarro-Silva |
| author_facet | Oscar Alexander Aguirre-Obando Anna Julia Pietrobon Ana Caroline Dalla Bona Mário Antônio Navarro-Silva |
| author_sort | Oscar Alexander Aguirre-Obando |
| collection | DOAJ |
| description | After a dengue outbreak, the knowledge on the extent, distribution and mechanisms of insecticide resistance is essential for successful insecticide-based dengue control interventions. Therefore, we evaluated the potential changes to insecticide resistance in natural Aedes aegypti populations to Organophosphates (OP) and Pyrethroids (PY) after chemical vector control interventions. After a Dengue outbreak in 2010, A. aegypti mosquitoes from the urban area of Jacarezinho (Paraná, Brazil) were collected in 2011 and 2012. Insecticide resistance to OP Temephos was assessed in 2011 and 2012 by dose–response bioassays adopting WHO-based protocols. Additionally, in both sampling, PY resistance was also investigated by the Val1016Ile mutation genotyping. In 2011, a random collection of mosquitoes was carried out; while in 2012, the urban area was divided into four regions where mosquitoes were sampled randomly. Bioassays conducted with larvae in 2011 (82 ± 10%; RR95 = 3.6) and 2012 (95 ± 3%; RR95 = 2.5) indicated an incipient altered susceptibility to Temephos. On the other hand, the Val1016IIe mutation analysis in 2011, presented frequencies of the 1016Ilekdr allele equal to 80%. Nevertheless, in 2012, when the urban area of Jacarezinho was analyzed as a single unit, the frequency of the mutant allele was 70%. Additionally, the distribution analysis of the Val1016Ile mutation in 2012 showed the mutant allele frequencies ≥60% in all regions. These outcomes indicated the necessity of developing alternative strategies such as insecticide rotations for delaying the evolution of resistance. Keywords: Bioassays, Dengue, Insecticide resistance, Knockdown resistance, Pyrethroids, Temephos |
| first_indexed | 2024-12-10T14:37:54Z |
| format | Article |
| id | doaj.art-397953aa75324839adf42e0a71e28cc1 |
| institution | Directory Open Access Journal |
| issn | 0085-5626 |
| language | English |
| last_indexed | 2024-12-10T14:37:54Z |
| publishDate | 2016-01-01 |
| publisher | Sociedade Brasileira de Entomologia |
| record_format | Article |
| series | Revista Brasileira de Entomologia |
| spelling | doaj.art-397953aa75324839adf42e0a71e28cc12022-12-22T01:44:47ZengSociedade Brasileira de EntomologiaRevista Brasileira de Entomologia0085-56262016-01-0160194100Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue OutbreakOscar Alexander Aguirre-Obando0Anna Julia Pietrobon1Ana Caroline Dalla Bona2Mário Antônio Navarro-Silva3Laboratory of Medical and Veterinary Entomology, Department of Zoology, Universidade Federal do Paraná, Curitiba, PR, BrazilLaboratory of Medical and Veterinary Entomology, Department of Zoology, Universidade Federal do Paraná, Curitiba, PR, BrazilLaboratory of Medical and Veterinary Entomology, Department of Zoology, Universidade Federal do Paraná, Curitiba, PR, BrazilCorresponding author.; Laboratory of Medical and Veterinary Entomology, Department of Zoology, Universidade Federal do Paraná, Curitiba, PR, BrazilAfter a dengue outbreak, the knowledge on the extent, distribution and mechanisms of insecticide resistance is essential for successful insecticide-based dengue control interventions. Therefore, we evaluated the potential changes to insecticide resistance in natural Aedes aegypti populations to Organophosphates (OP) and Pyrethroids (PY) after chemical vector control interventions. After a Dengue outbreak in 2010, A. aegypti mosquitoes from the urban area of Jacarezinho (Paraná, Brazil) were collected in 2011 and 2012. Insecticide resistance to OP Temephos was assessed in 2011 and 2012 by dose–response bioassays adopting WHO-based protocols. Additionally, in both sampling, PY resistance was also investigated by the Val1016Ile mutation genotyping. In 2011, a random collection of mosquitoes was carried out; while in 2012, the urban area was divided into four regions where mosquitoes were sampled randomly. Bioassays conducted with larvae in 2011 (82 ± 10%; RR95 = 3.6) and 2012 (95 ± 3%; RR95 = 2.5) indicated an incipient altered susceptibility to Temephos. On the other hand, the Val1016IIe mutation analysis in 2011, presented frequencies of the 1016Ilekdr allele equal to 80%. Nevertheless, in 2012, when the urban area of Jacarezinho was analyzed as a single unit, the frequency of the mutant allele was 70%. Additionally, the distribution analysis of the Val1016Ile mutation in 2012 showed the mutant allele frequencies ≥60% in all regions. These outcomes indicated the necessity of developing alternative strategies such as insecticide rotations for delaying the evolution of resistance. Keywords: Bioassays, Dengue, Insecticide resistance, Knockdown resistance, Pyrethroids, Temephoshttp://www.sciencedirect.com/science/article/pii/S0085562615001351 |
| spellingShingle | Oscar Alexander Aguirre-Obando Anna Julia Pietrobon Ana Caroline Dalla Bona Mário Antônio Navarro-Silva Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue Outbreak Revista Brasileira de Entomologia |
| title | Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue Outbreak |
| title_full | Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue Outbreak |
| title_fullStr | Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue Outbreak |
| title_full_unstemmed | Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue Outbreak |
| title_short | Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in Aedes aegypti populations from Jacarezinho (Brazil) after a Dengue Outbreak |
| title_sort | contrasting patterns of insecticide resistance and knockdown resistance kdr in aedes aegypti populations from jacarezinho brazil after a dengue outbreak |
| url | http://www.sciencedirect.com/science/article/pii/S0085562615001351 |
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