Protocol for constructing large size human antibody heavy chain variable domain (VH) library and selection of SARS-CoV-2 neutralizing antibody domains

Protocol for constructing large size human antibody heavy chain variable domain (VH) library and selection of SARS-CoV-2 neutralizing antibody domains

Summary: This protocol is a comprehensive guide to phage display-based selection of virus neutralizing VH antibody domains. It details three optimized parts including (1) construction of a large-sized (theoretically > 1011) naïve human antibody heavy chain domain library, (2) SARS-CoV-2 antigen e...

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Bibliographic Details
Main Authors:	Chuan Chen, Zehua Sun, Xianglei Liu, Wei Li, Dimiter S. Dimitrov
Format:	Article
Language:	English
Published:	Elsevier 2021-09-01
Series:	STAR Protocols
Subjects:	Cell culture High Throughput Screening Immunology Microbiology Molecular Biology Antibody
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721003245

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