Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (<i>Litopenaeus vannamei</i>) under Low Salinity
β-Glucan could significantly improve the antioxidant capacity of aquatic animals. The effects of different dietary levels (0 (control), 0.05, 0.1, 0.2 or 0.4%) of β-glucan on the growth, survival, antioxidant capacity, immunity, intestinal microbiota and transcriptional responses of <i>Litopen...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/11/11/2282 |
| _version_ | 1797466030188003328 |
|---|---|
| author | Yanbing Qiao Li Zhou Yayu Qu Kunyu Lu Fenglu Han Erchao Li |
| author_facet | Yanbing Qiao Li Zhou Yayu Qu Kunyu Lu Fenglu Han Erchao Li |
| author_sort | Yanbing Qiao |
| collection | DOAJ |
| description | β-Glucan could significantly improve the antioxidant capacity of aquatic animals. The effects of different dietary levels (0 (control), 0.05, 0.1, 0.2 or 0.4%) of β-glucan on the growth, survival, antioxidant capacity, immunity, intestinal microbiota and transcriptional responses of <i>Litopenaeus vannamei</i> under low salinity (≤3) were investigated. The dietary growth trial lasted 35 days (initial shrimp 0.26 ± 0.01 g). The results indicated that the growth performance of the 0.1% and 0.2% groups was significantly better than that of the control group. A second-order polynomial regression analysis of growth performance against dietary β-glucan indicated that the optimal dietary β-glucan level was 0.2% of dry matter. The digestive enzyme activity of the hepatopancreas was enhanced with increasing β-glucan levels. The antioxidant and nonspecific immunity capacities of the hepatopancreas were also enhanced in the 0.1% group. The α-diversity index analysis of the intestinal microbiota showed that the intestinal microbial richness of <i>L. vannamei</i> increased in the 0.1% group. The relative abundance of Proteobacteria decreased in the 0.1% group compared with the control group. The transcriptome results indicate that the prebiotic mechanisms of β-glucan include upregulating the expression of nonspecific immune genes and osmoregulation genes and activating KEGG pathways associated with carbohydrate metabolism under low-salinity stress. These results suggested that dietary supplementation with β-glucan markedly increased growth performance and alleviated the negative effects of low-salinity stress by contributing to the activity of biochemical enzymes and enriching carbohydrate metabolism in <i>L. vannamei</i>. |
| first_indexed | 2024-03-09T18:31:03Z |
| format | Article |
| id | doaj.art-397aeb06f9ac48eaa36b30a8c3833fbe |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T18:31:03Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-397aeb06f9ac48eaa36b30a8c3833fbe2023-11-24T07:32:42ZengMDPI AGAntioxidants2076-39212022-11-011111228210.3390/antiox11112282Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (<i>Litopenaeus vannamei</i>) under Low SalinityYanbing Qiao0Li Zhou1Yayu Qu2Kunyu Lu3Fenglu Han4Erchao Li5Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou 570228, Chinaβ-Glucan could significantly improve the antioxidant capacity of aquatic animals. The effects of different dietary levels (0 (control), 0.05, 0.1, 0.2 or 0.4%) of β-glucan on the growth, survival, antioxidant capacity, immunity, intestinal microbiota and transcriptional responses of <i>Litopenaeus vannamei</i> under low salinity (≤3) were investigated. The dietary growth trial lasted 35 days (initial shrimp 0.26 ± 0.01 g). The results indicated that the growth performance of the 0.1% and 0.2% groups was significantly better than that of the control group. A second-order polynomial regression analysis of growth performance against dietary β-glucan indicated that the optimal dietary β-glucan level was 0.2% of dry matter. The digestive enzyme activity of the hepatopancreas was enhanced with increasing β-glucan levels. The antioxidant and nonspecific immunity capacities of the hepatopancreas were also enhanced in the 0.1% group. The α-diversity index analysis of the intestinal microbiota showed that the intestinal microbial richness of <i>L. vannamei</i> increased in the 0.1% group. The relative abundance of Proteobacteria decreased in the 0.1% group compared with the control group. The transcriptome results indicate that the prebiotic mechanisms of β-glucan include upregulating the expression of nonspecific immune genes and osmoregulation genes and activating KEGG pathways associated with carbohydrate metabolism under low-salinity stress. These results suggested that dietary supplementation with β-glucan markedly increased growth performance and alleviated the negative effects of low-salinity stress by contributing to the activity of biochemical enzymes and enriching carbohydrate metabolism in <i>L. vannamei</i>.https://www.mdpi.com/2076-3921/11/11/2282<i>Litopenaeus vannamei</i>aquaculturelow salinityβ-glucantranscriptome |
| spellingShingle | Yanbing Qiao Li Zhou Yayu Qu Kunyu Lu Fenglu Han Erchao Li Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (<i>Litopenaeus vannamei</i>) under Low Salinity Antioxidants <i>Litopenaeus vannamei</i> aquaculture low salinity β-glucan transcriptome |
| title | Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (<i>Litopenaeus vannamei</i>) under Low Salinity |
| title_full | Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (<i>Litopenaeus vannamei</i>) under Low Salinity |
| title_fullStr | Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (<i>Litopenaeus vannamei</i>) under Low Salinity |
| title_full_unstemmed | Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (<i>Litopenaeus vannamei</i>) under Low Salinity |
| title_short | Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (<i>Litopenaeus vannamei</i>) under Low Salinity |
| title_sort | effects of different dietary β glucan levels on antioxidant capacity and immunity gut microbiota and transcriptome responses of white shrimp i litopenaeus vannamei i under low salinity |
| topic | <i>Litopenaeus vannamei</i> aquaculture low salinity β-glucan transcriptome |
| url | https://www.mdpi.com/2076-3921/11/11/2282 |
| work_keys_str_mv | AT yanbingqiao effectsofdifferentdietarybglucanlevelsonantioxidantcapacityandimmunitygutmicrobiotaandtranscriptomeresponsesofwhiteshrimpilitopenaeusvannameiiunderlowsalinity AT lizhou effectsofdifferentdietarybglucanlevelsonantioxidantcapacityandimmunitygutmicrobiotaandtranscriptomeresponsesofwhiteshrimpilitopenaeusvannameiiunderlowsalinity AT yayuqu effectsofdifferentdietarybglucanlevelsonantioxidantcapacityandimmunitygutmicrobiotaandtranscriptomeresponsesofwhiteshrimpilitopenaeusvannameiiunderlowsalinity AT kunyulu effectsofdifferentdietarybglucanlevelsonantioxidantcapacityandimmunitygutmicrobiotaandtranscriptomeresponsesofwhiteshrimpilitopenaeusvannameiiunderlowsalinity AT fengluhan effectsofdifferentdietarybglucanlevelsonantioxidantcapacityandimmunitygutmicrobiotaandtranscriptomeresponsesofwhiteshrimpilitopenaeusvannameiiunderlowsalinity AT erchaoli effectsofdifferentdietarybglucanlevelsonantioxidantcapacityandimmunitygutmicrobiotaandtranscriptomeresponsesofwhiteshrimpilitopenaeusvannameiiunderlowsalinity |