Gene expression and cellular changes in injured myocardium of Ciona intestinalis
Ciona intestinalis is an invertebrate animal model system that is well characterized and has many advantages for the study of cardiovascular biology. The regulatory mechanisms of cardiac myocyte proliferation in Ciona are intriguing since regeneration of functional tissue has been demonstrated in ot...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2024.1304755/full |
| _version_ | 1797263654286000128 |
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| author | Serenity Stokes Pooja Pardhanani Palmer Jeremy L. Barth Robert L. Price Bella G. Parker Heather J. Evans Anderson |
| author_facet | Serenity Stokes Pooja Pardhanani Palmer Jeremy L. Barth Robert L. Price Bella G. Parker Heather J. Evans Anderson |
| author_sort | Serenity Stokes |
| collection | DOAJ |
| description | Ciona intestinalis is an invertebrate animal model system that is well characterized and has many advantages for the study of cardiovascular biology. The regulatory mechanisms of cardiac myocyte proliferation in Ciona are intriguing since regeneration of functional tissue has been demonstrated in other organs of Ciona in response to injury. To identify genes that are differentially expressed in response to Ciona cardiac injury, microarray analysis was conducted on RNA from adult Ciona hearts with normal or damaged myocardium. After a 24- or 48-h recovery period, total RNA was isolated from damaged and control hearts. Initial results indicate significant changes in gene expression in hearts damaged by ligation in comparison to control hearts. Ligation injury shows differential expression of 223 genes as compared to control with limited false discovery (5.8%). Among these 223 genes, 117 have known human orthologs of which 68 were upregulated and 49 were downregulated. Notably, Fgf9/16/20, insulin-like growth factor binding protein and Ras-related protein Rab11b were significantly upregulated in injured hearts, whereas expression of a junctophilin ortholog was decreased. Histological analyses of injured myocardium were conducted in parallel to the microarray study which revealed thickened myocardium in injured hearts. Taken together, these studies will connect differences in gene expression to cellular changes in the myocardium of Ciona, which will help to promote further investigations into the regulatory mechanisms of cardiac myocyte proliferation across chordates. |
| first_indexed | 2024-04-25T00:16:26Z |
| format | Article |
| id | doaj.art-397b4463b33d415b9a747262d602f938 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-25T00:16:26Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-397b4463b33d415b9a747262d602f9382024-03-13T04:18:38ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2024-03-011210.3389/fcell.2024.13047551304755Gene expression and cellular changes in injured myocardium of Ciona intestinalisSerenity Stokes0Pooja Pardhanani Palmer1Jeremy L. Barth2Robert L. Price3Bella G. Parker4Heather J. Evans Anderson5Central Piedmont Community College, Natural Sciences Division, Charlotte, NC, United StatesAtrium Health, Division of Community and Social Impact, Department of Community Health, Charlotte, NC, United StatesDepartment of Regenerative Medicine and Cell Biology, Medical University of South Carolina Proteogenomics Facility, Charleston, SC, United StatesDepartment of Cell Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC, United StatesDepartment of Biology, Stetson University, DeLand, FL, United StatesDepartment of Health and Biomedical Sciences, Advent Health University, Orlando, FL, United StatesCiona intestinalis is an invertebrate animal model system that is well characterized and has many advantages for the study of cardiovascular biology. The regulatory mechanisms of cardiac myocyte proliferation in Ciona are intriguing since regeneration of functional tissue has been demonstrated in other organs of Ciona in response to injury. To identify genes that are differentially expressed in response to Ciona cardiac injury, microarray analysis was conducted on RNA from adult Ciona hearts with normal or damaged myocardium. After a 24- or 48-h recovery period, total RNA was isolated from damaged and control hearts. Initial results indicate significant changes in gene expression in hearts damaged by ligation in comparison to control hearts. Ligation injury shows differential expression of 223 genes as compared to control with limited false discovery (5.8%). Among these 223 genes, 117 have known human orthologs of which 68 were upregulated and 49 were downregulated. Notably, Fgf9/16/20, insulin-like growth factor binding protein and Ras-related protein Rab11b were significantly upregulated in injured hearts, whereas expression of a junctophilin ortholog was decreased. Histological analyses of injured myocardium were conducted in parallel to the microarray study which revealed thickened myocardium in injured hearts. Taken together, these studies will connect differences in gene expression to cellular changes in the myocardium of Ciona, which will help to promote further investigations into the regulatory mechanisms of cardiac myocyte proliferation across chordates.https://www.frontiersin.org/articles/10.3389/fcell.2024.1304755/fullCiona intestinalismyocardiumgene expressioncardiac myocytemicroarray |
| spellingShingle | Serenity Stokes Pooja Pardhanani Palmer Jeremy L. Barth Robert L. Price Bella G. Parker Heather J. Evans Anderson Gene expression and cellular changes in injured myocardium of Ciona intestinalis Frontiers in Cell and Developmental Biology Ciona intestinalis myocardium gene expression cardiac myocyte microarray |
| title | Gene expression and cellular changes in injured myocardium of Ciona intestinalis |
| title_full | Gene expression and cellular changes in injured myocardium of Ciona intestinalis |
| title_fullStr | Gene expression and cellular changes in injured myocardium of Ciona intestinalis |
| title_full_unstemmed | Gene expression and cellular changes in injured myocardium of Ciona intestinalis |
| title_short | Gene expression and cellular changes in injured myocardium of Ciona intestinalis |
| title_sort | gene expression and cellular changes in injured myocardium of ciona intestinalis |
| topic | Ciona intestinalis myocardium gene expression cardiac myocyte microarray |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2024.1304755/full |
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