The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA Clone
Viral diseases of cultivated crops are often caused by virus spillover from wild plants. Tobacco (<i>N</i><i>. tabacum</i>) is an important economic crop grown globally. The viral pathogens of tobacco are traditional major subjects in virology studies and key considerations i...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/10/1467 |
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| author | Jinlong Yin Xin Hong Sha Luo Jingquan Tan Yuanming Zhang Yanglin Qiu Muhammad Faizan Latif Tao Gao Haijia Yu Jingke Bai Shujun Li Kai Xu |
| author_facet | Jinlong Yin Xin Hong Sha Luo Jingquan Tan Yuanming Zhang Yanglin Qiu Muhammad Faizan Latif Tao Gao Haijia Yu Jingke Bai Shujun Li Kai Xu |
| author_sort | Jinlong Yin |
| collection | DOAJ |
| description | Viral diseases of cultivated crops are often caused by virus spillover from wild plants. Tobacco (<i>N</i><i>. tabacum</i>) is an important economic crop grown globally. The viral pathogens of tobacco are traditional major subjects in virology studies and key considerations in tobacco breeding practices. A positive-strand RNA virus, wild tomato mosaic virus (WTMV), belonging to the genus <i>potyvirus</i> in the family <i>potyviridae</i> was recently found to infect tobacco in China. In this study, diseased tobacco leaf samples were collected in the Henan Province of China during 2020–2021. Several samples from different locations were identified as WTMV positive. An infectious DNA clone was constructed based on one of the WTMV isolates. By using this clone, we found that WTMV from tobacco could establish infections on natural reservoir hosts, demonstrating a possible route of WTMV spillover and overwintering in the tobacco field. Furthermore, the WTMV infection was found to be accompanied by other tobacco viruses in the field. The co-inoculation experiments indicate the superinfection exclusion (SIE) between WTMV and other <i>potyvirus</i> species that infect tobacco. Overall, our work reveals novel aspects of WTMV evolution and infection in tobacco and provides an important tool for further studies of WTMV. |
| first_indexed | 2024-03-09T20:39:39Z |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T20:39:39Z |
| publishDate | 2022-10-01 |
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| series | Biology |
| spelling | doaj.art-397bbabc063a4b1fb63b8a9c4b05e1d62023-11-23T23:00:02ZengMDPI AGBiology2079-77372022-10-011110146710.3390/biology11101467The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA CloneJinlong Yin0Xin Hong1Sha Luo2Jingquan Tan3Yuanming Zhang4Yanglin Qiu5Muhammad Faizan Latif6Tao Gao7Haijia Yu8Jingke Bai9Shujun Li10Kai Xu11Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaKey Laboratory for Green Preservation & Control of Tobacco Diseases and Pests in Huanghuai Growing Area, Tobacco Research Institute, Henan Academy of Agricultural Sciences, Xuchang 461000, ChinaKey Laboratory for Green Preservation & Control of Tobacco Diseases and Pests in Huanghuai Growing Area, Tobacco Research Institute, Henan Academy of Agricultural Sciences, Xuchang 461000, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaViral diseases of cultivated crops are often caused by virus spillover from wild plants. Tobacco (<i>N</i><i>. tabacum</i>) is an important economic crop grown globally. The viral pathogens of tobacco are traditional major subjects in virology studies and key considerations in tobacco breeding practices. A positive-strand RNA virus, wild tomato mosaic virus (WTMV), belonging to the genus <i>potyvirus</i> in the family <i>potyviridae</i> was recently found to infect tobacco in China. In this study, diseased tobacco leaf samples were collected in the Henan Province of China during 2020–2021. Several samples from different locations were identified as WTMV positive. An infectious DNA clone was constructed based on one of the WTMV isolates. By using this clone, we found that WTMV from tobacco could establish infections on natural reservoir hosts, demonstrating a possible route of WTMV spillover and overwintering in the tobacco field. Furthermore, the WTMV infection was found to be accompanied by other tobacco viruses in the field. The co-inoculation experiments indicate the superinfection exclusion (SIE) between WTMV and other <i>potyvirus</i> species that infect tobacco. Overall, our work reveals novel aspects of WTMV evolution and infection in tobacco and provides an important tool for further studies of WTMV.https://www.mdpi.com/2079-7737/11/10/1467wild tomato mosaic virustobaccosuperinfection exclusionoverwinter host |
| spellingShingle | Jinlong Yin Xin Hong Sha Luo Jingquan Tan Yuanming Zhang Yanglin Qiu Muhammad Faizan Latif Tao Gao Haijia Yu Jingke Bai Shujun Li Kai Xu The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA Clone Biology wild tomato mosaic virus tobacco superinfection exclusion overwinter host |
| title | The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA Clone |
| title_full | The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA Clone |
| title_fullStr | The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA Clone |
| title_full_unstemmed | The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA Clone |
| title_short | The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA Clone |
| title_sort | characterization of the tobacco derived wild tomato mosaic virus by employing its infectious dna clone |
| topic | wild tomato mosaic virus tobacco superinfection exclusion overwinter host |
| url | https://www.mdpi.com/2079-7737/11/10/1467 |
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