Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5°C, -3°C and -16°C for two weeks. The amount of hydrogen peroxide (H2O2),...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2009-07-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-200903-0017_Potential_role_of_hydrogen_peroxide_and_melanin_in_the_cold_hardiness_of_Ostrinia_nubilalis_Lepidoptera_Pyral.php |
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| author | Danijela KOJIC Ivan SPASOJEVIC Miloš MOJOVIC Duško BLAGOJEVIC M. WORLAND Gordana GRUBOR-LAJSIC Mihajlo B. SPASIC |
| author_facet | Danijela KOJIC Ivan SPASOJEVIC Miloš MOJOVIC Duško BLAGOJEVIC M. WORLAND Gordana GRUBOR-LAJSIC Mihajlo B. SPASIC |
| author_sort | Danijela KOJIC |
| collection | DOAJ |
| description | The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5°C, -3°C and -16°C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3°C than at 5°C and -16°C. At the same temperature (-3°C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3°C, which is the temperature that triggers freezing tolerance, is suggested. |
| first_indexed | 2024-12-20T12:46:22Z |
| format | Article |
| id | doaj.art-397c8667e0344be681d2e1227ea8b2bd |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-20T12:46:22Z |
| publishDate | 2009-07-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-397c8667e0344be681d2e1227ea8b2bd2022-12-21T19:40:18ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292009-07-01106345145410.14411/eje.2009.056eje-200903-0017Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)Danijela KOJIC0Ivan SPASOJEVIC1Miloš MOJOVIC2Duško BLAGOJEVIC3M. WORLAND4Gordana GRUBOR-LAJSIC5Mihajlo B. SPASIC6Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad, Serbia; e-mail: gordanagl@ib.ns.ac.yuCenter for Multidisciplinary Studies, University of Belgrade, SerbiaFaculty of Physical Chemistry, University of Belgrade, SerbiaDepartment of Physiology, Institute for Biological Research "Sinisa Stankovic", Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaBritish Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge CB3 0ET, UKDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad, Serbia; e-mail: gordanagl@ib.ns.ac.yuDepartment of Physiology, Institute for Biological Research "Sinisa Stankovic", Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaThe aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5°C, -3°C and -16°C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3°C than at 5°C and -16°C. At the same temperature (-3°C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3°C, which is the temperature that triggers freezing tolerance, is suggested.https://www.eje.cz/artkey/eje-200903-0017_Potential_role_of_hydrogen_peroxide_and_melanin_in_the_cold_hardiness_of_Ostrinia_nubilalis_Lepidoptera_Pyral.phpostrinia nubilalisantioxidant enzymesmelaninhydrogen peroxidecold-hardiness |
| spellingShingle | Danijela KOJIC Ivan SPASOJEVIC Miloš MOJOVIC Duško BLAGOJEVIC M. WORLAND Gordana GRUBOR-LAJSIC Mihajlo B. SPASIC Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae) European Journal of Entomology ostrinia nubilalis antioxidant enzymes melanin hydrogen peroxide cold-hardiness |
| title | Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae) |
| title_full | Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae) |
| title_fullStr | Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae) |
| title_full_unstemmed | Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae) |
| title_short | Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae) |
| title_sort | potential role of hydrogen peroxide and melanin in the cold hardiness of ostrinia nubilalis lepidoptera pyralidae |
| topic | ostrinia nubilalis antioxidant enzymes melanin hydrogen peroxide cold-hardiness |
| url | https://www.eje.cz/artkey/eje-200903-0017_Potential_role_of_hydrogen_peroxide_and_melanin_in_the_cold_hardiness_of_Ostrinia_nubilalis_Lepidoptera_Pyral.php |
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