JVG9, a benzimidazole derivative, alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes
Trypanosoma cruzi has a particular cytoskeleton that consists of a subpellicular network of microtubules and actin microfilaments. Therefore, it is an excellent target for the development of new anti-parasitic drugs. Benzimidazole 2-carbamates, a class of well-known broad-spectrum anthelmintics, hav...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2014-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600757&lng=en&tlng=en |
| _version_ | 1797757246640226304 |
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| author | Dylan L Díaz-Chiguer Francisco Hernández-Luis Benjamín Nogueda-Torres Rafael Castillo Olivia Reynoso-Ducoing Alicia Hernández-Campos Javier R Ambrosio |
| author_facet | Dylan L Díaz-Chiguer Francisco Hernández-Luis Benjamín Nogueda-Torres Rafael Castillo Olivia Reynoso-Ducoing Alicia Hernández-Campos Javier R Ambrosio |
| author_sort | Dylan L Díaz-Chiguer |
| collection | DOAJ |
| description | Trypanosoma cruzi has a particular cytoskeleton that consists of a subpellicular network of microtubules and actin microfilaments. Therefore, it is an excellent target for the development of new anti-parasitic drugs. Benzimidazole 2-carbamates, a class of well-known broad-spectrum anthelmintics, have been shown to inhibit the in vitro growth of many protozoa. Therefore, to find efficient anti-trypanosomal (trypanocidal) drugs, our group has designed and synthesised several benzimidazole derivatives. One, named JVG9 (5-chloro-1H-benzimidazole-2-thiol), has been found to be effective against T. cruzi bloodstream trypomastigotes under both in vitro and in vivo conditions. Here, we present the in vitro effects observed by laser scanning confocal and scanning electron microscopy on T. cruzi trypomastigotes. Changes in the surface and the distribution of the cytoskeletal proteins are consistent with the hypothesis that the trypanocidal activity of JVG9 involves the cytoskeleton as a target. |
| first_indexed | 2024-03-12T18:13:07Z |
| format | Article |
| id | doaj.art-39855a11b3a2402fbdb7ca3fa04d71b6 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:13:07Z |
| publishDate | 2014-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-39855a11b3a2402fbdb7ca3fa04d71b62023-08-02T09:14:08ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-09-01109675776010.1590/0074-0276140096S0074-02762014000600757JVG9, a benzimidazole derivative, alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotesDylan L Díaz-ChiguerFrancisco Hernández-LuisBenjamín Nogueda-TorresRafael CastilloOlivia Reynoso-DucoingAlicia Hernández-CamposJavier R AmbrosioTrypanosoma cruzi has a particular cytoskeleton that consists of a subpellicular network of microtubules and actin microfilaments. Therefore, it is an excellent target for the development of new anti-parasitic drugs. Benzimidazole 2-carbamates, a class of well-known broad-spectrum anthelmintics, have been shown to inhibit the in vitro growth of many protozoa. Therefore, to find efficient anti-trypanosomal (trypanocidal) drugs, our group has designed and synthesised several benzimidazole derivatives. One, named JVG9 (5-chloro-1H-benzimidazole-2-thiol), has been found to be effective against T. cruzi bloodstream trypomastigotes under both in vitro and in vivo conditions. Here, we present the in vitro effects observed by laser scanning confocal and scanning electron microscopy on T. cruzi trypomastigotes. Changes in the surface and the distribution of the cytoskeletal proteins are consistent with the hypothesis that the trypanocidal activity of JVG9 involves the cytoskeleton as a target.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600757&lng=en&tlng=enTrypanosoma cruzibenzimidazolecytoskeletontubulinactin |
| spellingShingle | Dylan L Díaz-Chiguer Francisco Hernández-Luis Benjamín Nogueda-Torres Rafael Castillo Olivia Reynoso-Ducoing Alicia Hernández-Campos Javier R Ambrosio JVG9, a benzimidazole derivative, alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes Memorias do Instituto Oswaldo Cruz Trypanosoma cruzi benzimidazole cytoskeleton tubulin actin |
| title | JVG9, a benzimidazole derivative, alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes |
| title_full | JVG9, a benzimidazole derivative, alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes |
| title_fullStr | JVG9, a benzimidazole derivative, alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes |
| title_full_unstemmed | JVG9, a benzimidazole derivative, alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes |
| title_short | JVG9, a benzimidazole derivative, alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes |
| title_sort | jvg9 a benzimidazole derivative alters the surface and cytoskeleton of trypanosoma cruzi bloodstream trypomastigotes |
| topic | Trypanosoma cruzi benzimidazole cytoskeleton tubulin actin |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600757&lng=en&tlng=en |
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