Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish
This study aimed to obtain three Trp-containing peptides from β-lactoglobulin and study their effects on anxiety-like behaviors in zebrafish. Three Trp-containing peptides were prepared from β-lactoglobulin by selective enzymatic hydrolysis and identified by UPLC-Q-TOF MS/MS. The anxiety-like behavi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1100718/full |
| _version_ | 1797959721535143936 |
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| author | Xiping Zhu Dan Xie Qiong Zhu Yufeng Li Chun Cui Chun Cui |
| author_facet | Xiping Zhu Dan Xie Qiong Zhu Yufeng Li Chun Cui Chun Cui |
| author_sort | Xiping Zhu |
| collection | DOAJ |
| description | This study aimed to obtain three Trp-containing peptides from β-lactoglobulin and study their effects on anxiety-like behaviors in zebrafish. Three Trp-containing peptides were prepared from β-lactoglobulin by selective enzymatic hydrolysis and identified by UPLC-Q-TOF MS/MS. The anxiety-like behaviors of zebrafish were reduced after two weeks of administrated of β-lactoglobulin Trp peptides (LAWP), VAGTWY, VAGTW and G TW(concentration of 56 μg/mL or 500 μg/mL). As an index of serotonergic activity, we assessed the enhancing abilities of 5-HT synthesis. The treatment remarkably enhanced the 5-HT synthesis by upregulation of Trp concentration and Trp hydroxylase activation. In addition, this study further validated the anti-anxiety effects of whey protein hydrolysate with a high Trp index in animal and the experimental results were consistent with those reported in previous studies. Our results showed that β-lactoglobulin Trp peptides ingestion has a significant anti-anxiety effect as evidenced by the increasing Trp concentration, TPH activation and 5-HT level compared to the control group, with the VAGTW being the more effective. |
| first_indexed | 2024-04-11T00:35:44Z |
| format | Article |
| id | doaj.art-3985c18c14644a53a32731313305bb27 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T00:35:44Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-3985c18c14644a53a32731313305bb272023-01-06T18:24:51ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-01-01910.3389/fnut.2022.11007181100718Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in ZebrafishXiping Zhu0Dan Xie1Qiong Zhu2Yufeng Li3Chun Cui4Chun Cui5College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, ChinaCollege of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, ChinaCollege of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, ChinaCollege of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou, ChinaResearch Institute for Food Nutrition and Human Health, Guangzhou, ChinaThis study aimed to obtain three Trp-containing peptides from β-lactoglobulin and study their effects on anxiety-like behaviors in zebrafish. Three Trp-containing peptides were prepared from β-lactoglobulin by selective enzymatic hydrolysis and identified by UPLC-Q-TOF MS/MS. The anxiety-like behaviors of zebrafish were reduced after two weeks of administrated of β-lactoglobulin Trp peptides (LAWP), VAGTWY, VAGTW and G TW(concentration of 56 μg/mL or 500 μg/mL). As an index of serotonergic activity, we assessed the enhancing abilities of 5-HT synthesis. The treatment remarkably enhanced the 5-HT synthesis by upregulation of Trp concentration and Trp hydroxylase activation. In addition, this study further validated the anti-anxiety effects of whey protein hydrolysate with a high Trp index in animal and the experimental results were consistent with those reported in previous studies. Our results showed that β-lactoglobulin Trp peptides ingestion has a significant anti-anxiety effect as evidenced by the increasing Trp concentration, TPH activation and 5-HT level compared to the control group, with the VAGTW being the more effective.https://www.frontiersin.org/articles/10.3389/fnut.2022.1100718/fullβ-lactoglobulintryptophan peptideszebrafishbehaviorserotonin |
| spellingShingle | Xiping Zhu Dan Xie Qiong Zhu Yufeng Li Chun Cui Chun Cui Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish Frontiers in Nutrition β-lactoglobulin tryptophan peptides zebrafish behavior serotonin |
| title | Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish |
| title_full | Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish |
| title_fullStr | Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish |
| title_full_unstemmed | Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish |
| title_short | Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish |
| title_sort | preparation of β lactoglobulin derived tryptophan peptide and its effect on anxiety like behaviors in zebrafish |
| topic | β-lactoglobulin tryptophan peptides zebrafish behavior serotonin |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1100718/full |
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