Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins
The aim of this study was to investigate the synergistic antioxidant potential and protective effect of grape seed procyanidins (GSP) in combination with Auricularia auricular-judae polysaccharides (AAP IV) on radiation injury in splenocytes. Rat splenocyte irradiation resulted in significantly high...
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MDPI AG
2014-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/12/20675 |
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| author | Haina Bai Zhenyu Wang Jie Cui Keli Yun Hua Zhang Rui Hai Liu Ziluan Fan Cuilin Cheng |
| author_facet | Haina Bai Zhenyu Wang Jie Cui Keli Yun Hua Zhang Rui Hai Liu Ziluan Fan Cuilin Cheng |
| author_sort | Haina Bai |
| collection | DOAJ |
| description | The aim of this study was to investigate the synergistic antioxidant potential and protective effect of grape seed procyanidins (GSP) in combination with Auricularia auricular-judae polysaccharides (AAP IV) on radiation injury in splenocytes. Rat splenocyte irradiation resulted in significantly higher apoptosis rate, malondialdehyde (MDA) (p < 0.005), reactive oxygen species (ROS) (p < 0.01); cell viability, total superoxide dismutase (T-SOD) (p < 0.01), catalase (CAT) (p < 0.01), glutathione peroxidase (GSH-PX) (p < 0.05), activity and glutathione (GSH) (p < 0.01) levels were significantly reduced, compared with the control group. “GSP + AAP IV” treatment of rat splenocytes at doses of “GSP (0.3 μg/mL) + AAP IV (50 μg/mL)” displayed higher radioprotective and antioxidative effects than the administration of either GSP or AAP IV, as evident by lower levels of MDA (p < 0.001) concentration, as well as higher cell viability and T-SOD (p < 0.05), CAT (p < 0.005), GSH-PX (p < 0.01) and GSH content compared to the radiation group. In addition, in vivo studies have shown that “GSP + AAP IV” significantly ameliorated the decrease of spleen index (p < 0.005) and spleen GSH (p < 0.005) levels and significantly inhibited the increase of MDA (p < 0.005) levels of spleen with radiation-induced damage, compared with the non-treated group. The in vivo and in vitro results suggested that GSP and AAP IV have a synergistic protective effect against radiation-induced injury by improving the antioxidant and immunomodulation activities. |
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| language | English |
| last_indexed | 2024-04-13T01:29:42Z |
| publishDate | 2014-12-01 |
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| series | Molecules |
| spelling | doaj.art-3987125dcd51431bb6714c12c8be5c322022-12-22T03:08:32ZengMDPI AGMolecules1420-30492014-12-011912206752069410.3390/molecules191220675molecules191220675Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed ProcyanidinsHaina Bai0Zhenyu Wang1Jie Cui2Keli Yun3Hua Zhang4Rui Hai Liu5Ziluan Fan6Cuilin Cheng7School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, ChinaDepartment of Food Science, Cornell University, Ithaca, NY 14853, USASchool of Forestry, Northeast Forestry University, 26 HeXing Road, DongLi District, Harbin 150040, ChinaSchool of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, ChinaThe aim of this study was to investigate the synergistic antioxidant potential and protective effect of grape seed procyanidins (GSP) in combination with Auricularia auricular-judae polysaccharides (AAP IV) on radiation injury in splenocytes. Rat splenocyte irradiation resulted in significantly higher apoptosis rate, malondialdehyde (MDA) (p < 0.005), reactive oxygen species (ROS) (p < 0.01); cell viability, total superoxide dismutase (T-SOD) (p < 0.01), catalase (CAT) (p < 0.01), glutathione peroxidase (GSH-PX) (p < 0.05), activity and glutathione (GSH) (p < 0.01) levels were significantly reduced, compared with the control group. “GSP + AAP IV” treatment of rat splenocytes at doses of “GSP (0.3 μg/mL) + AAP IV (50 μg/mL)” displayed higher radioprotective and antioxidative effects than the administration of either GSP or AAP IV, as evident by lower levels of MDA (p < 0.001) concentration, as well as higher cell viability and T-SOD (p < 0.05), CAT (p < 0.005), GSH-PX (p < 0.01) and GSH content compared to the radiation group. In addition, in vivo studies have shown that “GSP + AAP IV” significantly ameliorated the decrease of spleen index (p < 0.005) and spleen GSH (p < 0.005) levels and significantly inhibited the increase of MDA (p < 0.005) levels of spleen with radiation-induced damage, compared with the non-treated group. The in vivo and in vitro results suggested that GSP and AAP IV have a synergistic protective effect against radiation-induced injury by improving the antioxidant and immunomodulation activities.http://www.mdpi.com/1420-3049/19/12/20675grape seed procyanidinsAuricularia auricular-judae polysaccharidesradiation protectivesynergisticsplenocytes |
| spellingShingle | Haina Bai Zhenyu Wang Jie Cui Keli Yun Hua Zhang Rui Hai Liu Ziluan Fan Cuilin Cheng Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins Molecules grape seed procyanidins Auricularia auricular-judae polysaccharides radiation protective synergistic splenocytes |
| title | Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins |
| title_full | Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins |
| title_fullStr | Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins |
| title_full_unstemmed | Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins |
| title_short | Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins |
| title_sort | synergistic radiation protective effect of purified auricularia auricular judae polysaccharide aap iv with grape seed procyanidins |
| topic | grape seed procyanidins Auricularia auricular-judae polysaccharides radiation protective synergistic splenocytes |
| url | http://www.mdpi.com/1420-3049/19/12/20675 |
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