The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality
Abstract Background Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1389-0 |
| _version_ | 1818216590829682688 |
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| author | Jane-Fang Yu Yu-Hua Lai Tse-En Wang Yu-Syuan Wei Yu-Jia Chang Sheng-Hsiang Li Shih-Chien Chin Radhika Joshi Hui-Wen Chang Pei-Shiue Tsai |
| author_facet | Jane-Fang Yu Yu-Hua Lai Tse-En Wang Yu-Syuan Wei Yu-Jia Chang Sheng-Hsiang Li Shih-Chien Chin Radhika Joshi Hui-Wen Chang Pei-Shiue Tsai |
| author_sort | Jane-Fang Yu |
| collection | DOAJ |
| description | Abstract Background Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes for sperm preparation. In this study, we investigated whether collagenase can be used to degelify the semen plug and accelerate the semen liquefaction process in zoo captive chimpanzee species (Pan troglodytes). Results We showed that incubation of chimpanzee ejaculates with 0.1% type I collagenase efficiently and significantly (p < 0.05) releases 2.7-fold more spermatozoa from the coagulated ejaculates, and this degelification process did not alter sperm morphology or viability; nor did it stimulate spontaneous capacitation or an acrosome reaction as assessed by tyrosine phosphorylation and peanut agglutinin stains; moreover, based on computer assisted sperm analysis assay, motility-related parameters remained similar to those of untreated spermatozoa. When collagenase effects were evaluated on cryopreserved sperm samples, we observed post collagenase treatment in which 2.5% glycerol, as a cryoprotectant, preserved sperm acrosome integrity better than 7.8%; however, 7.8% glycerol, as a cryoprotectant, maintained sperm motility better than that of 2.5% glycerol. Conclusions Our results demonstrated for the first time that type I collagenase can be used to obtain a significantly higher number of spermatozoa from colloid chimpanzee semen ejaculate without affecting the physiological properties of spermatozoa, and these results are critical for the subsequent gamete development. Our results would benefit sperm preparation processes and cryopreservation efficiency per ejaculate, as more unaffected spermatozoa can be released from the semen plug within a shorter period of time. These results would also benefit the genetic diversity of the chimpanzee species, using sperm cells from less dominant individuals, and for achieving better pregnancy success in primates with significantly higher amounts of sperm for artificial insemination. |
| first_indexed | 2024-12-12T06:54:24Z |
| format | Article |
| id | doaj.art-398b070a299249de96dac864778d319c |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-12T06:54:24Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-398b070a299249de96dac864778d319c2022-12-22T00:33:59ZengBMCBMC Veterinary Research1746-61482018-02-0114111210.1186/s12917-018-1389-0The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm qualityJane-Fang Yu0Yu-Hua Lai1Tse-En Wang2Yu-Syuan Wei3Yu-Jia Chang4Sheng-Hsiang Li5Shih-Chien Chin6Radhika Joshi7Hui-Wen Chang8Pei-Shiue Tsai9Conservation and Research Center, Taipei ZooDepartment of Veterinary Medicine, National Taiwan UniversityDepartment of Veterinary Medicine, National Taiwan UniversityDepartment of Veterinary Medicine, National Taiwan UniversityConservation and Research Center, Taipei ZooDepartment of Medical Research, Mackay Memorial HospitalConservation and Research Center, Taipei ZooDepartment of Veterinary Medicine, National Taiwan UniversityDepartment of Veterinary Medicine, National Taiwan UniversityDepartment of Veterinary Medicine, National Taiwan UniversityAbstract Background Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes for sperm preparation. In this study, we investigated whether collagenase can be used to degelify the semen plug and accelerate the semen liquefaction process in zoo captive chimpanzee species (Pan troglodytes). Results We showed that incubation of chimpanzee ejaculates with 0.1% type I collagenase efficiently and significantly (p < 0.05) releases 2.7-fold more spermatozoa from the coagulated ejaculates, and this degelification process did not alter sperm morphology or viability; nor did it stimulate spontaneous capacitation or an acrosome reaction as assessed by tyrosine phosphorylation and peanut agglutinin stains; moreover, based on computer assisted sperm analysis assay, motility-related parameters remained similar to those of untreated spermatozoa. When collagenase effects were evaluated on cryopreserved sperm samples, we observed post collagenase treatment in which 2.5% glycerol, as a cryoprotectant, preserved sperm acrosome integrity better than 7.8%; however, 7.8% glycerol, as a cryoprotectant, maintained sperm motility better than that of 2.5% glycerol. Conclusions Our results demonstrated for the first time that type I collagenase can be used to obtain a significantly higher number of spermatozoa from colloid chimpanzee semen ejaculate without affecting the physiological properties of spermatozoa, and these results are critical for the subsequent gamete development. Our results would benefit sperm preparation processes and cryopreservation efficiency per ejaculate, as more unaffected spermatozoa can be released from the semen plug within a shorter period of time. These results would also benefit the genetic diversity of the chimpanzee species, using sperm cells from less dominant individuals, and for achieving better pregnancy success in primates with significantly higher amounts of sperm for artificial insemination.http://link.springer.com/article/10.1186/s12917-018-1389-0CollagenaseSperm preservationGenetic diversityChimpanzee |
| spellingShingle | Jane-Fang Yu Yu-Hua Lai Tse-En Wang Yu-Syuan Wei Yu-Jia Chang Sheng-Hsiang Li Shih-Chien Chin Radhika Joshi Hui-Wen Chang Pei-Shiue Tsai The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality BMC Veterinary Research Collagenase Sperm preservation Genetic diversity Chimpanzee |
| title | The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality |
| title_full | The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality |
| title_fullStr | The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality |
| title_full_unstemmed | The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality |
| title_short | The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality |
| title_sort | effects of type i collagenase on the degelification of chimpanzee pan troglodytes semen plug and sperm quality |
| topic | Collagenase Sperm preservation Genetic diversity Chimpanzee |
| url | http://link.springer.com/article/10.1186/s12917-018-1389-0 |
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