Porcine Sapelovirus 3Cpro Inhibits the Production of Type I Interferon
Porcine sapelovirus (PSV) is the causative pathogen of reproductive obstacles, acute diarrhea, respiratory distress, or severe polioencephalomyelitis in swine. Nevertheless, the pathogenicity and pathogenic mechanism of PSV infection are not fully understood, which hinders disease prevention and con...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.852473/full |
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| author | Mengge Yin Mengge Yin Wei Wen Wei Wen Haoyuan Wang Haoyuan Wang Qiongqiong Zhao Qiongqiong Zhao Hechao Zhu Hechao Zhu Huanchun Chen Huanchun Chen Huanchun Chen Huanchun Chen Xiangmin Li Xiangmin Li Xiangmin Li Xiangmin Li Ping Qian Ping Qian Ping Qian Ping Qian |
| author_facet | Mengge Yin Mengge Yin Wei Wen Wei Wen Haoyuan Wang Haoyuan Wang Qiongqiong Zhao Qiongqiong Zhao Hechao Zhu Hechao Zhu Huanchun Chen Huanchun Chen Huanchun Chen Huanchun Chen Xiangmin Li Xiangmin Li Xiangmin Li Xiangmin Li Ping Qian Ping Qian Ping Qian Ping Qian |
| author_sort | Mengge Yin |
| collection | DOAJ |
| description | Porcine sapelovirus (PSV) is the causative pathogen of reproductive obstacles, acute diarrhea, respiratory distress, or severe polioencephalomyelitis in swine. Nevertheless, the pathogenicity and pathogenic mechanism of PSV infection are not fully understood, which hinders disease prevention and control. In this study, we found that PSV was sensitive to type I interferon (IFN-β). However, PSV could not activate the IFN-β promoter and induce IFN-β mRNA expression, indicating that PSV has evolved an effective mechanism to block IFN-β production. Further study showed that PSV inhibited the production of IFN-β by cleaving mitochondrial antiviral signaling (MAVS) and degrading melanoma differentiation-associated gene 5 (MDA5) and TANK-binding kinase 1 (TBK1) through viral 3Cpro. In addition, our study demonstrated that PSV 3Cpro degrades MDA5 and TBK1 through its protease activity and cleaves MAVS through the caspase pathway. Collectively, our results revealed that PSV inhibits the production of type I interferon to escape host antiviral immunity through cleaving and degrading the adaptor molecules. |
| first_indexed | 2024-12-12T03:35:32Z |
| format | Article |
| id | doaj.art-398b1d0a3e5049aa957e081f56d76bc9 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-12T03:35:32Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-398b1d0a3e5049aa957e081f56d76bc92022-12-22T00:39:49ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-06-011210.3389/fcimb.2022.852473852473Porcine Sapelovirus 3Cpro Inhibits the Production of Type I InterferonMengge Yin0Mengge Yin1Wei Wen2Wei Wen3Haoyuan Wang4Haoyuan Wang5Qiongqiong Zhao6Qiongqiong Zhao7Hechao Zhu8Hechao Zhu9Huanchun Chen10Huanchun Chen11Huanchun Chen12Huanchun Chen13Xiangmin Li14Xiangmin Li15Xiangmin Li16Xiangmin Li17Ping Qian18Ping Qian19Ping Qian20Ping Qian21State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaKey Laboratory of Development of Veterinary Diagnostic ProductsAgriculture of the People’s Republic of China, Ministry of Agriculture of the People’s Republic of China, Wuhan, ChinaInternational Research Center for Animal DiseaseTechnology of the People’s Republic of China, Ministry of Science and Technology of the People’s Republic of China, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaKey Laboratory of Development of Veterinary Diagnostic ProductsAgriculture of the People’s Republic of China, Ministry of Agriculture of the People’s Republic of China, Wuhan, ChinaInternational Research Center for Animal DiseaseTechnology of the People’s Republic of China, Ministry of Science and Technology of the People’s Republic of China, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaKey Laboratory of Development of Veterinary Diagnostic ProductsAgriculture of the People’s Republic of China, Ministry of Agriculture of the People’s Republic of China, Wuhan, ChinaInternational Research Center for Animal DiseaseTechnology of the People’s Republic of China, Ministry of Science and Technology of the People’s Republic of China, Wuhan, ChinaPorcine sapelovirus (PSV) is the causative pathogen of reproductive obstacles, acute diarrhea, respiratory distress, or severe polioencephalomyelitis in swine. Nevertheless, the pathogenicity and pathogenic mechanism of PSV infection are not fully understood, which hinders disease prevention and control. In this study, we found that PSV was sensitive to type I interferon (IFN-β). However, PSV could not activate the IFN-β promoter and induce IFN-β mRNA expression, indicating that PSV has evolved an effective mechanism to block IFN-β production. Further study showed that PSV inhibited the production of IFN-β by cleaving mitochondrial antiviral signaling (MAVS) and degrading melanoma differentiation-associated gene 5 (MDA5) and TANK-binding kinase 1 (TBK1) through viral 3Cpro. In addition, our study demonstrated that PSV 3Cpro degrades MDA5 and TBK1 through its protease activity and cleaves MAVS through the caspase pathway. Collectively, our results revealed that PSV inhibits the production of type I interferon to escape host antiviral immunity through cleaving and degrading the adaptor molecules.https://www.frontiersin.org/articles/10.3389/fcimb.2022.852473/fullporcine sapelovirus3C proteaseMAVSTBK1MDA5 |
| spellingShingle | Mengge Yin Mengge Yin Wei Wen Wei Wen Haoyuan Wang Haoyuan Wang Qiongqiong Zhao Qiongqiong Zhao Hechao Zhu Hechao Zhu Huanchun Chen Huanchun Chen Huanchun Chen Huanchun Chen Xiangmin Li Xiangmin Li Xiangmin Li Xiangmin Li Ping Qian Ping Qian Ping Qian Ping Qian Porcine Sapelovirus 3Cpro Inhibits the Production of Type I Interferon Frontiers in Cellular and Infection Microbiology porcine sapelovirus 3C protease MAVS TBK1 MDA5 |
| title | Porcine Sapelovirus 3Cpro Inhibits the Production of Type I Interferon |
| title_full | Porcine Sapelovirus 3Cpro Inhibits the Production of Type I Interferon |
| title_fullStr | Porcine Sapelovirus 3Cpro Inhibits the Production of Type I Interferon |
| title_full_unstemmed | Porcine Sapelovirus 3Cpro Inhibits the Production of Type I Interferon |
| title_short | Porcine Sapelovirus 3Cpro Inhibits the Production of Type I Interferon |
| title_sort | porcine sapelovirus 3cpro inhibits the production of type i interferon |
| topic | porcine sapelovirus 3C protease MAVS TBK1 MDA5 |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.852473/full |
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