Comparison of Enzyme-Linked Immunosorbent Assay and Immunochromatography for Rotavirus Detection in Children Below Five Years with Acute Gastroenteritis
Background: Group-A rotaviruses are responsible for 30 to 60% of severe watery diarrhea cases in young children. Timely diagnosis of rotavirus infection helps to determine appropriate treatment and prevents unnecessary use of antibiotics. Aim: To compare Immunochromatography (ICG) with standard...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6425/14049_CE[Ra1]_F(AK)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
| Summary: | Background: Group-A rotaviruses are responsible for 30 to
60% of severe watery diarrhea cases in young children. Timely
diagnosis of rotavirus infection helps to determine appropriate
treatment and prevents unnecessary use of antibiotics.
Aim: To compare Immunochromatography (ICG) with standard
ELISA test for diagnosis of and to determine incidence,
clinical socio-epidemiological profile and possible risk factors
associated with rotavirus infection in children below five years
with acute gastroenteritis.
Settings and Design: A prospective study performed from
February 2013 to April 2014 in Microbiology and Paediatrics
Departments, Government Medical College, Amritsar, Punjab,
India.
Materials and Methods: Hundred stool samples from children
below five years diagnosed with acute gastroenteritis were
taken and tested by ICG and standard ELISA test.
Statistical analysis: Performed using the SPSS software for
Windows, version 17.0. P-values calculated using χ2
test for
categorical variables. A p < 0.05 was considered significant.
Results: Maximum cases with ICG showed a sensitivity of
95.24% and specificity of 97.47% when compared to ELISA.
Incidence of rotavirus diarrhea was 21% using ELISA and 23%
using ICG. With ELISA rotavirus infection was highest in age
group 6 months to 24 months (83.3%) and in male (90.47%).
The infection was maximum during November to April and
presented with triad of diarrhea, vomiting, fever (76.2%).
Majority of cases had watery diarrhea in high percentage
(90.47%). Severe dehydration (76.19%), respiratory symptoms
(38.09%), bottle feeding (52.38%), malnourished children
(47.61%), children playing with toys (47.6%) and submersible
water pump (61.95%) as a source of drinking water associated
with rotavirus infection were found to statistically significant.
Conclusion: ICG shows a good agreement with ELISA and has
the advantage of being a quicker, cost-effective and useful for
testing single specimen, convenient, not requiring additional
equipment, readily available, simple to perform and easy-toread results. |
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| ISSN: | 2249-782X 0973-709X |