Variability in Nutrient Use by Orchid Mycorrhizal Fungi in Two Medium Types
Orchid mycorrhizal fungi (OMF) from the rhizoctonia aggregate are generally considered to be soil saprotrophs, but their ability to utilize various nutrient sources has been studied in a limited number of isolates cultivated predominantly in liquid media, although rhizoctonia typically grow on the s...
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MDPI AG
2023-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/1/88 |
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| author | Alžběta Novotná Sophie Mennicken Caio C. Pires de Paula Hélène Vogt-Schilb Milan Kotilínek Tamara Těšitelová Petr Šmilauer Jana Jersáková |
| author_facet | Alžběta Novotná Sophie Mennicken Caio C. Pires de Paula Hélène Vogt-Schilb Milan Kotilínek Tamara Těšitelová Petr Šmilauer Jana Jersáková |
| author_sort | Alžběta Novotná |
| collection | DOAJ |
| description | Orchid mycorrhizal fungi (OMF) from the rhizoctonia aggregate are generally considered to be soil saprotrophs, but their ability to utilize various nutrient sources has been studied in a limited number of isolates cultivated predominantly in liquid media, although rhizoctonia typically grow on the surface of solid substrates. Nine isolates representing the key OMF families (Ceratobasidiaceae, Tulasnellaceae and Serendipitaceae), sampled in Southern France and the Czech Republic, were tested for their ability to utilize carbon (C), nitrogen (N) and phosphorus (P) sources <i>in vitro</i> in both liquid and solid media. The isolates showed significant inter- and intra-familiar variability in nutrient utilization, most notably in N sources. Isolates produced generally larger amounts of dry biomass on solid medium than in liquid one, but some isolates showed no or limited biomass production on solid medium with particular nutrient sources. The largest amount of biomass was produced by isolates from the family Ceratobasidiaceae on most sources in both medium types. The biomass production of Tulasnellaceae isolates was affected by their phylogenetic relatedness on all sources and medium types. The ability of isolates to utilize particular nutrients in a liquid medium but not a solid one should be considered when optimizing solid media for symbiotic orchid seed germination and in understanding of OMF functional traits under <i>in situ</i> conditions. |
| first_indexed | 2024-03-09T12:03:30Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T12:03:30Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-398db1d6bc664a4b85649ef80579d1a22023-11-30T23:00:09ZengMDPI AGJournal of Fungi2309-608X2023-01-01918810.3390/jof9010088Variability in Nutrient Use by Orchid Mycorrhizal Fungi in Two Medium TypesAlžběta Novotná0Sophie Mennicken1Caio C. Pires de Paula2Hélène Vogt-Schilb3Milan Kotilínek4Tamara Těšitelová5Petr Šmilauer6Jana Jersáková7Department of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech RepublicDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech RepublicDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech RepublicDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech RepublicDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech RepublicDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech RepublicDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech RepublicDepartment of Ecosystem Biology, Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech RepublicOrchid mycorrhizal fungi (OMF) from the rhizoctonia aggregate are generally considered to be soil saprotrophs, but their ability to utilize various nutrient sources has been studied in a limited number of isolates cultivated predominantly in liquid media, although rhizoctonia typically grow on the surface of solid substrates. Nine isolates representing the key OMF families (Ceratobasidiaceae, Tulasnellaceae and Serendipitaceae), sampled in Southern France and the Czech Republic, were tested for their ability to utilize carbon (C), nitrogen (N) and phosphorus (P) sources <i>in vitro</i> in both liquid and solid media. The isolates showed significant inter- and intra-familiar variability in nutrient utilization, most notably in N sources. Isolates produced generally larger amounts of dry biomass on solid medium than in liquid one, but some isolates showed no or limited biomass production on solid medium with particular nutrient sources. The largest amount of biomass was produced by isolates from the family Ceratobasidiaceae on most sources in both medium types. The biomass production of Tulasnellaceae isolates was affected by their phylogenetic relatedness on all sources and medium types. The ability of isolates to utilize particular nutrients in a liquid medium but not a solid one should be considered when optimizing solid media for symbiotic orchid seed germination and in understanding of OMF functional traits under <i>in situ</i> conditions.https://www.mdpi.com/2309-608X/9/1/88nutrient utilizationOrchidaceaerhizoctoniasolid mediumliquid mediumTulasnellaceae |
| spellingShingle | Alžběta Novotná Sophie Mennicken Caio C. Pires de Paula Hélène Vogt-Schilb Milan Kotilínek Tamara Těšitelová Petr Šmilauer Jana Jersáková Variability in Nutrient Use by Orchid Mycorrhizal Fungi in Two Medium Types Journal of Fungi nutrient utilization Orchidaceae rhizoctonia solid medium liquid medium Tulasnellaceae |
| title | Variability in Nutrient Use by Orchid Mycorrhizal Fungi in Two Medium Types |
| title_full | Variability in Nutrient Use by Orchid Mycorrhizal Fungi in Two Medium Types |
| title_fullStr | Variability in Nutrient Use by Orchid Mycorrhizal Fungi in Two Medium Types |
| title_full_unstemmed | Variability in Nutrient Use by Orchid Mycorrhizal Fungi in Two Medium Types |
| title_short | Variability in Nutrient Use by Orchid Mycorrhizal Fungi in Two Medium Types |
| title_sort | variability in nutrient use by orchid mycorrhizal fungi in two medium types |
| topic | nutrient utilization Orchidaceae rhizoctonia solid medium liquid medium Tulasnellaceae |
| url | https://www.mdpi.com/2309-608X/9/1/88 |
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