A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis.
Here we report the isolation of a murine model for heritable T cell lymphoblastic leukemia/lymphoma (T-ALL) called Spontaneous dominant leukemia (Sdl). Sdl heterozygous mice develop disease with a short latency and high penetrance, while mice homozygous for the mutation die early during embryonic de...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003034&type=printable |
| _version_ | 1826799264466993152 |
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| author | Bruce N Bagley Thomas M Keane Vilena I Maklakova Jonathon G Marshall Rachael A Lester Michelle M Cancel Alex R Paulsen Laura E Bendzick Raha A Been Scott C Kogan Robert T Cormier Christina Kendziorski David J Adams Lara S Collier |
| author_facet | Bruce N Bagley Thomas M Keane Vilena I Maklakova Jonathon G Marshall Rachael A Lester Michelle M Cancel Alex R Paulsen Laura E Bendzick Raha A Been Scott C Kogan Robert T Cormier Christina Kendziorski David J Adams Lara S Collier |
| author_sort | Bruce N Bagley |
| collection | DOAJ |
| description | Here we report the isolation of a murine model for heritable T cell lymphoblastic leukemia/lymphoma (T-ALL) called Spontaneous dominant leukemia (Sdl). Sdl heterozygous mice develop disease with a short latency and high penetrance, while mice homozygous for the mutation die early during embryonic development. Sdl mice exhibit an increase in the frequency of micronucleated reticulocytes, and T-ALLs from Sdl mice harbor small amplifications and deletions, including activating deletions at the Notch1 locus. Using exome sequencing it was determined that Sdl mice harbor a spontaneously acquired mutation in Mcm4 (Mcm4(D573H)). MCM4 is part of the heterohexameric complex of MCM2-7 that is important for licensing of DNA origins prior to S phase and also serves as the core of the replicative helicase that unwinds DNA at replication forks. Previous studies in murine models have discovered that genetic reductions of MCM complex levels promote tumor formation by causing genomic instability. However, Sdl mice possess normal levels of Mcms, and there is no evidence for loss-of-heterozygosity at the Mcm4 locus in Sdl leukemias. Studies in Saccharomyces cerevisiae indicate that the Sdl mutation produces a biologically inactive helicase. Together, these data support a model in which chromosomal abnormalities in Sdl mice result from the ability of MCM4(D573H) to incorporate into MCM complexes and render them inactive. Our studies indicate that dominantly acting alleles of MCMs can be compatible with viability but have dramatic oncogenic consequences by causing chromosomal abnormalities. |
| first_indexed | 2024-12-21T15:46:22Z |
| format | Article |
| id | doaj.art-399200a5f44a4eb1af69a037ab3204f8 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2025-03-17T00:32:19Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-399200a5f44a4eb1af69a037ab3204f82025-02-21T05:34:22ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042012-01-01811e100303410.1371/journal.pgen.1003034A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis.Bruce N BagleyThomas M KeaneVilena I MaklakovaJonathon G MarshallRachael A LesterMichelle M CancelAlex R PaulsenLaura E BendzickRaha A BeenScott C KoganRobert T CormierChristina KendziorskiDavid J AdamsLara S CollierHere we report the isolation of a murine model for heritable T cell lymphoblastic leukemia/lymphoma (T-ALL) called Spontaneous dominant leukemia (Sdl). Sdl heterozygous mice develop disease with a short latency and high penetrance, while mice homozygous for the mutation die early during embryonic development. Sdl mice exhibit an increase in the frequency of micronucleated reticulocytes, and T-ALLs from Sdl mice harbor small amplifications and deletions, including activating deletions at the Notch1 locus. Using exome sequencing it was determined that Sdl mice harbor a spontaneously acquired mutation in Mcm4 (Mcm4(D573H)). MCM4 is part of the heterohexameric complex of MCM2-7 that is important for licensing of DNA origins prior to S phase and also serves as the core of the replicative helicase that unwinds DNA at replication forks. Previous studies in murine models have discovered that genetic reductions of MCM complex levels promote tumor formation by causing genomic instability. However, Sdl mice possess normal levels of Mcms, and there is no evidence for loss-of-heterozygosity at the Mcm4 locus in Sdl leukemias. Studies in Saccharomyces cerevisiae indicate that the Sdl mutation produces a biologically inactive helicase. Together, these data support a model in which chromosomal abnormalities in Sdl mice result from the ability of MCM4(D573H) to incorporate into MCM complexes and render them inactive. Our studies indicate that dominantly acting alleles of MCMs can be compatible with viability but have dramatic oncogenic consequences by causing chromosomal abnormalities.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003034&type=printable |
| spellingShingle | Bruce N Bagley Thomas M Keane Vilena I Maklakova Jonathon G Marshall Rachael A Lester Michelle M Cancel Alex R Paulsen Laura E Bendzick Raha A Been Scott C Kogan Robert T Cormier Christina Kendziorski David J Adams Lara S Collier A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis. PLoS Genetics |
| title | A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis. |
| title_full | A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis. |
| title_fullStr | A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis. |
| title_full_unstemmed | A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis. |
| title_short | A dominantly acting murine allele of Mcm4 causes chromosomal abnormalities and promotes tumorigenesis. |
| title_sort | dominantly acting murine allele of mcm4 causes chromosomal abnormalities and promotes tumorigenesis |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003034&type=printable |
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