Pellets Inoculated with <i>Bacillus amyloliquefaciens</i> H57 Modulates Diet Preference and Rumen Factors Associated with Appetite Regulation in Steers
This study examined whether the probiotic <i>Bacillus amyloliquefaciens</i> strain H57 (H57) affects ruminal fermentation parameters that exercise post-ingestive feedback appetite control mechanisms. A 4 × 4 Latin square design was used to separate pre- and post-ingestive effects of H57...
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MDPI AG
2021-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/12/3455 |
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| author | Thi Thuy Ngo Nguyen N. Bang Peter Dart Matthew Callaghan Athol Klieve David McNeill |
| author_facet | Thi Thuy Ngo Nguyen N. Bang Peter Dart Matthew Callaghan Athol Klieve David McNeill |
| author_sort | Thi Thuy Ngo |
| collection | DOAJ |
| description | This study examined whether the probiotic <i>Bacillus amyloliquefaciens</i> strain H57 (H57) affects ruminal fermentation parameters that exercise post-ingestive feedback appetite control mechanisms. A 4 × 4 Latin square design was used to separate pre- and post-ingestive effects of H57 in four rumen-fistulated steers. The steers were offered a set amount of feedlot pellets, inoculated with H57 or without H57 (control, C). Half of the total amount of pellets fed were introduced intra-ruminally (r), and then the remaining pellets were orally consumed (o) to make four feeding treatments: H57r/H57o, H57r/Co, Cr/H57o and Cr/Co. Rumen fluid was sampled at 2, 4 and 6 h after feeding. Preference behaviour was tested immediately after the 6 h rumen fluid sampling by simultaneously offering the steers 4 kg of each of H57 and C pellets in adjacent troughs for 5 min. Steers preferred the pellets with added H57 over the C pellets (56:44; <i>p</i> < 0.001) and their preferences were not affected by the treatment protocol imposed to separate post- from pre-ingestive effects (<i>p</i> > 0.05). Steers fed H57 pellets had higher ruminal pH, molar proportions of iso-butyrate and iso-valerate (<i>p</i> < 0.05) and tended to have greater ruminal ammonia concentrations compared to those fed C pellets (<i>p</i> < 0.1). However, post-ingestive signals did not affect diet preference more than pre-ingestive signals. |
| first_indexed | 2024-03-10T04:39:21Z |
| format | Article |
| id | doaj.art-3995c508bb6747eaab67821547274d1d |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T04:39:21Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-3995c508bb6747eaab67821547274d1d2023-11-23T03:27:14ZengMDPI AGAnimals2076-26152021-12-011112345510.3390/ani11123455Pellets Inoculated with <i>Bacillus amyloliquefaciens</i> H57 Modulates Diet Preference and Rumen Factors Associated with Appetite Regulation in SteersThi Thuy Ngo0Nguyen N. Bang1Peter Dart2Matthew Callaghan3Athol Klieve4David McNeill5School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaSchool of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, AustraliaRidley AgriProducts Pty Ltd., Toowong, QLD 4066, AustraliaQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4069, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaThis study examined whether the probiotic <i>Bacillus amyloliquefaciens</i> strain H57 (H57) affects ruminal fermentation parameters that exercise post-ingestive feedback appetite control mechanisms. A 4 × 4 Latin square design was used to separate pre- and post-ingestive effects of H57 in four rumen-fistulated steers. The steers were offered a set amount of feedlot pellets, inoculated with H57 or without H57 (control, C). Half of the total amount of pellets fed were introduced intra-ruminally (r), and then the remaining pellets were orally consumed (o) to make four feeding treatments: H57r/H57o, H57r/Co, Cr/H57o and Cr/Co. Rumen fluid was sampled at 2, 4 and 6 h after feeding. Preference behaviour was tested immediately after the 6 h rumen fluid sampling by simultaneously offering the steers 4 kg of each of H57 and C pellets in adjacent troughs for 5 min. Steers preferred the pellets with added H57 over the C pellets (56:44; <i>p</i> < 0.001) and their preferences were not affected by the treatment protocol imposed to separate post- from pre-ingestive effects (<i>p</i> > 0.05). Steers fed H57 pellets had higher ruminal pH, molar proportions of iso-butyrate and iso-valerate (<i>p</i> < 0.05) and tended to have greater ruminal ammonia concentrations compared to those fed C pellets (<i>p</i> < 0.1). However, post-ingestive signals did not affect diet preference more than pre-ingestive signals.https://www.mdpi.com/2076-2615/11/12/3455<i>Bacillus amyloliquefaciens</i> H57feed preferenceruminal pHruminal VFAappetite |
| spellingShingle | Thi Thuy Ngo Nguyen N. Bang Peter Dart Matthew Callaghan Athol Klieve David McNeill Pellets Inoculated with <i>Bacillus amyloliquefaciens</i> H57 Modulates Diet Preference and Rumen Factors Associated with Appetite Regulation in Steers Animals <i>Bacillus amyloliquefaciens</i> H57 feed preference ruminal pH ruminal VFA appetite |
| title | Pellets Inoculated with <i>Bacillus amyloliquefaciens</i> H57 Modulates Diet Preference and Rumen Factors Associated with Appetite Regulation in Steers |
| title_full | Pellets Inoculated with <i>Bacillus amyloliquefaciens</i> H57 Modulates Diet Preference and Rumen Factors Associated with Appetite Regulation in Steers |
| title_fullStr | Pellets Inoculated with <i>Bacillus amyloliquefaciens</i> H57 Modulates Diet Preference and Rumen Factors Associated with Appetite Regulation in Steers |
| title_full_unstemmed | Pellets Inoculated with <i>Bacillus amyloliquefaciens</i> H57 Modulates Diet Preference and Rumen Factors Associated with Appetite Regulation in Steers |
| title_short | Pellets Inoculated with <i>Bacillus amyloliquefaciens</i> H57 Modulates Diet Preference and Rumen Factors Associated with Appetite Regulation in Steers |
| title_sort | pellets inoculated with i bacillus amyloliquefaciens i h57 modulates diet preference and rumen factors associated with appetite regulation in steers |
| topic | <i>Bacillus amyloliquefaciens</i> H57 feed preference ruminal pH ruminal VFA appetite |
| url | https://www.mdpi.com/2076-2615/11/12/3455 |
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