A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits
Abstract Background Autologous platelet concentrate has been widely used to encourage the regeneration of hard and soft tissues. Up to now, there are three generations of autologous platelet concentrates. Many studies have shown that the three autologous concentrates have different effects, but the...
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-022-02126-0 |
| _version_ | 1818356788437712896 |
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| author | Siying Li Hongyi Yang Qinyu Duan Hongyu Bao Aodi Li Wei Li Junliang Chen Yun He |
| author_facet | Siying Li Hongyi Yang Qinyu Duan Hongyu Bao Aodi Li Wei Li Junliang Chen Yun He |
| author_sort | Siying Li |
| collection | DOAJ |
| description | Abstract Background Autologous platelet concentrate has been widely used to encourage the regeneration of hard and soft tissues. Up to now, there are three generations of autologous platelet concentrates. Many studies have shown that the three autologous concentrates have different effects, but the specific diversities have not been studied. The purpose of this study was to explore and compare the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in New Zealand rabbits. Methods A total of 24 healthy male New Zealand white rabbits aged 8–12 weeks were selected. The experimental animals were randomly divided into four groups: three experimental groups were respectively implanted with PPP, CGF and PRF gel after bilateral mandibular anterior teeth were extracted, and the control group did not implant any material. The alveolar bone of the mandibular anterior region was taken at 2, 4 and 8 weeks after operation. The height and width of the extraction wound were detected by CBCT, the growth of the new bone was observed by HE and Masson staining, and the expression of osteogenic genes was detected by real-time PCR. Data were analyzed using IBM SPSS statistical package 22.0. Results The radiological results showed that alveolar bone resorption in all groups gradually increased over time. However, the experimental groups showed lower amounts of bone resorption. The histological results showed that new bone formation was observed in all groups. Over time, the new bone trabeculae of the CGF group became closely aligned while those in the PPP and PRF groups remained scattered. PCR results showed that the expression of BMP-2 and ALP was higher in the experimental groups than the control group. Conclusion In conclusion, the application of PRF, CGF and PPP in tooth extraction sockets effectively promoted bone regeneration. CGF showed more effective bone induction and tissue regeneration ability in the long term. |
| first_indexed | 2024-12-13T20:02:47Z |
| format | Article |
| id | doaj.art-399ab3cf8f124e7fb784cd4d319a1096 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-12-13T20:02:47Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-399ab3cf8f124e7fb784cd4d319a10962022-12-21T23:33:07ZengBMCBMC Oral Health1472-68312022-03-0122111310.1186/s12903-022-02126-0A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbitsSiying Li0Hongyi Yang1Qinyu Duan2Hongyu Bao3Aodi Li4Wei Li5Junliang Chen6Yun He7Oral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical UniversityOral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical UniversityOral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical UniversityOral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical UniversityOral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical UniversityOral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical UniversityOral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical UniversityOral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical UniversityAbstract Background Autologous platelet concentrate has been widely used to encourage the regeneration of hard and soft tissues. Up to now, there are three generations of autologous platelet concentrates. Many studies have shown that the three autologous concentrates have different effects, but the specific diversities have not been studied. The purpose of this study was to explore and compare the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in New Zealand rabbits. Methods A total of 24 healthy male New Zealand white rabbits aged 8–12 weeks were selected. The experimental animals were randomly divided into four groups: three experimental groups were respectively implanted with PPP, CGF and PRF gel after bilateral mandibular anterior teeth were extracted, and the control group did not implant any material. The alveolar bone of the mandibular anterior region was taken at 2, 4 and 8 weeks after operation. The height and width of the extraction wound were detected by CBCT, the growth of the new bone was observed by HE and Masson staining, and the expression of osteogenic genes was detected by real-time PCR. Data were analyzed using IBM SPSS statistical package 22.0. Results The radiological results showed that alveolar bone resorption in all groups gradually increased over time. However, the experimental groups showed lower amounts of bone resorption. The histological results showed that new bone formation was observed in all groups. Over time, the new bone trabeculae of the CGF group became closely aligned while those in the PPP and PRF groups remained scattered. PCR results showed that the expression of BMP-2 and ALP was higher in the experimental groups than the control group. Conclusion In conclusion, the application of PRF, CGF and PPP in tooth extraction sockets effectively promoted bone regeneration. CGF showed more effective bone induction and tissue regeneration ability in the long term.https://doi.org/10.1186/s12903-022-02126-0Tooth extractionPlatelet-rich fibrinOsteogenesisBone resorptionCone beam computed tomography |
| spellingShingle | Siying Li Hongyi Yang Qinyu Duan Hongyu Bao Aodi Li Wei Li Junliang Chen Yun He A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits BMC Oral Health Tooth extraction Platelet-rich fibrin Osteogenesis Bone resorption Cone beam computed tomography |
| title | A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits |
| title_full | A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits |
| title_fullStr | A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits |
| title_full_unstemmed | A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits |
| title_short | A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits |
| title_sort | comparative study of the effects of platelet rich fibrin concentrated growth factor and platelet poor plasma on the healing of tooth extraction sockets in rabbits |
| topic | Tooth extraction Platelet-rich fibrin Osteogenesis Bone resorption Cone beam computed tomography |
| url | https://doi.org/10.1186/s12903-022-02126-0 |
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