A halotolerant plant growth promoting consortium of Bacillus sp. RB3 and Pseudomonas sp. EB3 primes banana, Musa acuminata cv. Berangan, against salinity and Foc-TR4 stresses
A plant-growth promoting bacterial consortium of halotolerant Bacillus sp. and Pseudomonas sp. was evaluated for the ability to prime banana (Musa acuminata cv. Berangan) plants against abiotic (salinity) and biotic (Foc-TR4) stress challenges. PGPB consortium-primed banana plants showed better grow...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Current Plant Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662823000233 |
| _version_ | 1797390470787104768 |
|---|---|
| author | Abdussabur M. Kaleh Pooja Singh Purabi Mazumdar Gwo Rong Wong Kah Ooi Chua Jennifer Ann Harikrishna |
| author_facet | Abdussabur M. Kaleh Pooja Singh Purabi Mazumdar Gwo Rong Wong Kah Ooi Chua Jennifer Ann Harikrishna |
| author_sort | Abdussabur M. Kaleh |
| collection | DOAJ |
| description | A plant-growth promoting bacterial consortium of halotolerant Bacillus sp. and Pseudomonas sp. was evaluated for the ability to prime banana (Musa acuminata cv. Berangan) plants against abiotic (salinity) and biotic (Foc-TR4) stress challenges. PGPB consortium-primed banana plants showed better growth (plant height, root length and root biomass) and improved physiological parameters (relative water content, chlorophyll, and carotenoid contents) in both stressed conditions compared to non-primed banana plants. In addition, primed banana plants showed a reduction in lipid peroxidation and an increase in proline and antioxidant enzymatic activities (superoxide dismutase, catalase, ascorbate peroxidase, peroxidase, and glutathione reductase). In salt-stress conditions (sea salt at 100 mM NaCl equivalent), primed plants showed higher levels of K+, Ca2+, and Mg2+ and lower levels of Cl- and Na+ compared to non-primed salt-stressed plants. Under Foc-TR4 stress, the primed plants showed significant enhancement in the levels of total soluble phenolics, lignin content and defense-related enzyme activities (β-1, 3-glucanase, phenylalanine ammonia lyase, chitinase, polyphenol oxidase, and lipoxygenase). Expression analysis of nine stress-responsive MaWRKY genes (MaWRKY4, MaWRKY23, MaWRKY24, MaWRKY25, MaWRKY28, MaWRKY45, MaWRKY65, MaWRKY89 and MaWRKY145) showed significant upregulation in primed banana plants under salt and Foc-TR4 stresses compared to non-primed plants. These results demonstrate that inoculation with the PGPB consortium improved banana plant growth characteristics and primed WRKY-mediated protection against abiotic and biotic stresses. |
| first_indexed | 2024-03-08T23:11:15Z |
| format | Article |
| id | doaj.art-399af53d89c945f9b36f490ad1e0cffd |
| institution | Directory Open Access Journal |
| issn | 2214-6628 |
| language | English |
| last_indexed | 2024-03-08T23:11:15Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj.art-399af53d89c945f9b36f490ad1e0cffd2023-12-15T07:24:25ZengElsevierCurrent Plant Biology2214-66282023-09-0135100294A halotolerant plant growth promoting consortium of Bacillus sp. RB3 and Pseudomonas sp. EB3 primes banana, Musa acuminata cv. Berangan, against salinity and Foc-TR4 stressesAbdussabur M. Kaleh0Pooja Singh1Purabi Mazumdar2Gwo Rong Wong3Kah Ooi Chua4Jennifer Ann Harikrishna5Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur 50603, Malaysia; Institute of Biolocial Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, MalaysiaSchool of Science, Monash University Malaysia, 47500 Bandar Sunway, Selangor, MalaysiaCentre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur 50603, MalaysiaCentre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur 50603, MalaysiaCentre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur 50603, MalaysiaCentre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur 50603, Malaysia; Institute of Biolocial Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; Corresponding author at: Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur 50603, Malaysia.A plant-growth promoting bacterial consortium of halotolerant Bacillus sp. and Pseudomonas sp. was evaluated for the ability to prime banana (Musa acuminata cv. Berangan) plants against abiotic (salinity) and biotic (Foc-TR4) stress challenges. PGPB consortium-primed banana plants showed better growth (plant height, root length and root biomass) and improved physiological parameters (relative water content, chlorophyll, and carotenoid contents) in both stressed conditions compared to non-primed banana plants. In addition, primed banana plants showed a reduction in lipid peroxidation and an increase in proline and antioxidant enzymatic activities (superoxide dismutase, catalase, ascorbate peroxidase, peroxidase, and glutathione reductase). In salt-stress conditions (sea salt at 100 mM NaCl equivalent), primed plants showed higher levels of K+, Ca2+, and Mg2+ and lower levels of Cl- and Na+ compared to non-primed salt-stressed plants. Under Foc-TR4 stress, the primed plants showed significant enhancement in the levels of total soluble phenolics, lignin content and defense-related enzyme activities (β-1, 3-glucanase, phenylalanine ammonia lyase, chitinase, polyphenol oxidase, and lipoxygenase). Expression analysis of nine stress-responsive MaWRKY genes (MaWRKY4, MaWRKY23, MaWRKY24, MaWRKY25, MaWRKY28, MaWRKY45, MaWRKY65, MaWRKY89 and MaWRKY145) showed significant upregulation in primed banana plants under salt and Foc-TR4 stresses compared to non-primed plants. These results demonstrate that inoculation with the PGPB consortium improved banana plant growth characteristics and primed WRKY-mediated protection against abiotic and biotic stresses.http://www.sciencedirect.com/science/article/pii/S2214662823000233BananaFusariumPriming against stressPGPBSalinity stressWRKY |
| spellingShingle | Abdussabur M. Kaleh Pooja Singh Purabi Mazumdar Gwo Rong Wong Kah Ooi Chua Jennifer Ann Harikrishna A halotolerant plant growth promoting consortium of Bacillus sp. RB3 and Pseudomonas sp. EB3 primes banana, Musa acuminata cv. Berangan, against salinity and Foc-TR4 stresses Current Plant Biology Banana Fusarium Priming against stress PGPB Salinity stress WRKY |
| title | A halotolerant plant growth promoting consortium of Bacillus sp. RB3 and Pseudomonas sp. EB3 primes banana, Musa acuminata cv. Berangan, against salinity and Foc-TR4 stresses |
| title_full | A halotolerant plant growth promoting consortium of Bacillus sp. RB3 and Pseudomonas sp. EB3 primes banana, Musa acuminata cv. Berangan, against salinity and Foc-TR4 stresses |
| title_fullStr | A halotolerant plant growth promoting consortium of Bacillus sp. RB3 and Pseudomonas sp. EB3 primes banana, Musa acuminata cv. Berangan, against salinity and Foc-TR4 stresses |
| title_full_unstemmed | A halotolerant plant growth promoting consortium of Bacillus sp. RB3 and Pseudomonas sp. EB3 primes banana, Musa acuminata cv. Berangan, against salinity and Foc-TR4 stresses |
| title_short | A halotolerant plant growth promoting consortium of Bacillus sp. RB3 and Pseudomonas sp. EB3 primes banana, Musa acuminata cv. Berangan, against salinity and Foc-TR4 stresses |
| title_sort | halotolerant plant growth promoting consortium of bacillus sp rb3 and pseudomonas sp eb3 primes banana musa acuminata cv berangan against salinity and foc tr4 stresses |
| topic | Banana Fusarium Priming against stress PGPB Salinity stress WRKY |
| url | http://www.sciencedirect.com/science/article/pii/S2214662823000233 |
| work_keys_str_mv | AT abdussaburmkaleh ahalotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT poojasingh ahalotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT purabimazumdar ahalotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT gworongwong ahalotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT kahooichua ahalotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT jenniferannharikrishna ahalotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT abdussaburmkaleh halotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT poojasingh halotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT purabimazumdar halotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT gworongwong halotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT kahooichua halotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses AT jenniferannharikrishna halotolerantplantgrowthpromotingconsortiumofbacillussprb3andpseudomonasspeb3primesbananamusaacuminatacvberanganagainstsalinityandfoctr4stresses |