The Modulation of Functional Status of Bovine Spermatozoa by Progesterone
The aim of this study is to identify the effects of progesterone (PRG) on the capacitation and the acrosome reaction in bovine spermatozoa. The fresh sperm samples were incubated with and without capacitation inductors (heparin, dibutyryl cyclic adenosine monophosphate (dbcAMP)), hormones (prolactin...
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1788 |
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| author | Vitaly Denisenko Irena Chistyakova Natalia Volkova Ludmila Volkova Baylar Iolchiev Tatyana Kuzmina |
| author_facet | Vitaly Denisenko Irena Chistyakova Natalia Volkova Ludmila Volkova Baylar Iolchiev Tatyana Kuzmina |
| author_sort | Vitaly Denisenko |
| collection | DOAJ |
| description | The aim of this study is to identify the effects of progesterone (PRG) on the capacitation and the acrosome reaction in bovine spermatozoa. The fresh sperm samples were incubated with and without capacitation inductors (heparin, dibutyryl cyclic adenosine monophosphate (dbcAMP)), hormones (prolactin (PRL), PRG), inhibitors of microfilaments (cytochalasin D) and microtubules (nocodazole) during capacitation and acrosome reactions. The functional status of spermatozoa was examined using the chlortetracycline assay. Supplementation of heparin stimulated capacitation in the presence and absence of PRG. Cytochalasin D blocked the stimulating effect of heparin on capacitation. The addition of PRL during capacitation (without PRG) did not affect the functional status of spermatozoa, while in PRG-treated cells PRL stimulated the acrosome reaction. PRL (with and without PRG) increased the acrosome reaction in capacitated cells. These PRL-dependent effects were inhibited by nocodazole. During the acrosome reaction, in presence of dbcAMP, PRG decreased the proportion of acrosome-reacted cells compared to PRG-untreated cells. This effect in PRG-treated cells was canceled in the presence of nocodazole. In conclusion, PRG under the action of PRL and dbcAMP determines the changes in the functional status of native sperm cells, which indicates PRG modulating effect on the indicators of post-ejaculatory maturation of spermatozoa. |
| first_indexed | 2024-03-10T10:23:16Z |
| format | Article |
| id | doaj.art-399b42dd4b8c4d06bf502c60bec03c19 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:23:16Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-399b42dd4b8c4d06bf502c60bec03c192023-11-22T00:13:48ZengMDPI AGAnimals2076-26152021-06-01116178810.3390/ani11061788The Modulation of Functional Status of Bovine Spermatozoa by ProgesteroneVitaly Denisenko0Irena Chistyakova1Natalia Volkova2Ludmila Volkova3Baylar Iolchiev4Tatyana Kuzmina5Branch of Federal Research Center for Animal Husbandry Named after Academy Member L.K. Ernst, Russian Research Institute of Genetic and Breeding Farm Animals, 196601 Saint-Petersburg, RussiaBranch of Federal Research Center for Animal Husbandry Named after Academy Member L.K. Ernst, Russian Research Institute of Genetic and Breeding Farm Animals, 196601 Saint-Petersburg, RussiaFederal Research Center for Animal Husbandry Named after Academy Member L.K. Ernst, 142132 Moscow, RussiaFederal Research Center for Animal Husbandry Named after Academy Member L.K. Ernst, 142132 Moscow, RussiaFederal Research Center for Animal Husbandry Named after Academy Member L.K. Ernst, 142132 Moscow, RussiaBranch of Federal Research Center for Animal Husbandry Named after Academy Member L.K. Ernst, Russian Research Institute of Genetic and Breeding Farm Animals, 196601 Saint-Petersburg, RussiaThe aim of this study is to identify the effects of progesterone (PRG) on the capacitation and the acrosome reaction in bovine spermatozoa. The fresh sperm samples were incubated with and without capacitation inductors (heparin, dibutyryl cyclic adenosine monophosphate (dbcAMP)), hormones (prolactin (PRL), PRG), inhibitors of microfilaments (cytochalasin D) and microtubules (nocodazole) during capacitation and acrosome reactions. The functional status of spermatozoa was examined using the chlortetracycline assay. Supplementation of heparin stimulated capacitation in the presence and absence of PRG. Cytochalasin D blocked the stimulating effect of heparin on capacitation. The addition of PRL during capacitation (without PRG) did not affect the functional status of spermatozoa, while in PRG-treated cells PRL stimulated the acrosome reaction. PRL (with and without PRG) increased the acrosome reaction in capacitated cells. These PRL-dependent effects were inhibited by nocodazole. During the acrosome reaction, in presence of dbcAMP, PRG decreased the proportion of acrosome-reacted cells compared to PRG-untreated cells. This effect in PRG-treated cells was canceled in the presence of nocodazole. In conclusion, PRG under the action of PRL and dbcAMP determines the changes in the functional status of native sperm cells, which indicates PRG modulating effect on the indicators of post-ejaculatory maturation of spermatozoa.https://www.mdpi.com/2076-2615/11/6/1788progesteronebull spermatozoacapacitationacrosome reaction |
| spellingShingle | Vitaly Denisenko Irena Chistyakova Natalia Volkova Ludmila Volkova Baylar Iolchiev Tatyana Kuzmina The Modulation of Functional Status of Bovine Spermatozoa by Progesterone Animals progesterone bull spermatozoa capacitation acrosome reaction |
| title | The Modulation of Functional Status of Bovine Spermatozoa by Progesterone |
| title_full | The Modulation of Functional Status of Bovine Spermatozoa by Progesterone |
| title_fullStr | The Modulation of Functional Status of Bovine Spermatozoa by Progesterone |
| title_full_unstemmed | The Modulation of Functional Status of Bovine Spermatozoa by Progesterone |
| title_short | The Modulation of Functional Status of Bovine Spermatozoa by Progesterone |
| title_sort | modulation of functional status of bovine spermatozoa by progesterone |
| topic | progesterone bull spermatozoa capacitation acrosome reaction |
| url | https://www.mdpi.com/2076-2615/11/6/1788 |
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