Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat
Abstract Background Induction of mutation through chemical mutagenesis is a novel approach for preparing diverse germplasm. Introduction of functional alleles in the starch biosynthetic genes help in the improvement of the quality and yield of cereals. Results In the present study, a set of 350 stab...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04389-3 |
| _version_ | 1827875587031564288 |
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| author | Ahsan Irshad Huijun Guo Hongchun Xiong Yongdun Xie Hua Jin Jiayu Gu Chaojie Wang Liqun Yu Xianghui Wen Shirong Zhao Luxiang Liu |
| author_facet | Ahsan Irshad Huijun Guo Hongchun Xiong Yongdun Xie Hua Jin Jiayu Gu Chaojie Wang Liqun Yu Xianghui Wen Shirong Zhao Luxiang Liu |
| author_sort | Ahsan Irshad |
| collection | DOAJ |
| description | Abstract Background Induction of mutation through chemical mutagenesis is a novel approach for preparing diverse germplasm. Introduction of functional alleles in the starch biosynthetic genes help in the improvement of the quality and yield of cereals. Results In the present study, a set of 350 stable mutant lines were used to evaluate dynamic variation of the total starch contents. A megazyme kits were used for measuring the total starch content, resistant starch, amylose, and amylopectin content. Analysis of variance showed significant variation (p < 0.05) in starch content within the population. Furthermore, two high starch mutants (JE0173 and JE0218) and two low starch mutants (JE0089 and JE0418) were selected for studying different traits. A multiple comparison test showed that significant variation in all physiological and morphological traits, with respect to the parent variety (J411) in 2019–2020 and 2020–2021. The quantitative expression of starch metabolic genes revealed that eleven genes of JE0173 and twelve genes of JE0218 had consistent expression in high starch mutant lines. Similarly, in low starch mutant lines, eleven genes of JE0089 and thirteen genes of JE0418 had consistent expression in all stages of seed development. An additional two candidate genes showed over-expression (PHO1, PUL) in the high starch mutant lines, indicating that other starch metabolic genes may also contribute to the starch biosynthesis. The overexpression of SSII, SSIII and SBEI in JE0173 may be due to presence of missense mutations in these genes and SSI also showed overexpression which may be due to 3-primer_UTR variant. These mutations can affect the other starch related genes and help to increase the starch content in this mutant line (JE0173). Conclusions This study screened a large scale of mutant population and identified mutants, could provide useful genetic resources for the study of starch biosynthesis and genetic improvement of wheat in the future. Further study will help to understand new genes which are responsible for the fluctuation of total starch. |
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| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-12T17:09:03Z |
| publishDate | 2023-08-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-399d932d9222465bafd678b5ff790e222023-08-06T11:10:51ZengBMCBMC Plant Biology1471-22292023-08-0123111110.1186/s12870-023-04389-3Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheatAhsan Irshad0Huijun Guo1Hongchun Xiong2Yongdun Xie3Hua Jin4Jiayu Gu5Chaojie Wang6Liqun Yu7Xianghui Wen8Shirong Zhao9Luxiang Liu10Institute of Crop Sciences, National Engineering Laboratory of Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Centre of Space Mutagenesis for Crop ImprovementInstitute of Crop Sciences, National Engineering Laboratory of Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Centre of Space Mutagenesis for Crop ImprovementInstitute of Crop Sciences, National Engineering Laboratory of Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Centre of Space Mutagenesis for Crop ImprovementInstitute of Crop Sciences, National Engineering Laboratory of Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Centre of Space Mutagenesis for Crop ImprovementKey Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of TechnologyInstitute of Crop Sciences, National Engineering Laboratory of Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Centre of Space Mutagenesis for Crop ImprovementInstitute of Crop Sciences, National Engineering Laboratory of Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Centre of Space Mutagenesis for Crop ImprovementKey Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of TechnologyKey Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of TechnologyInstitute of Crop Sciences, National Engineering Laboratory of Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Centre of Space Mutagenesis for Crop ImprovementInstitute of Crop Sciences, National Engineering Laboratory of Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, National Centre of Space Mutagenesis for Crop ImprovementAbstract Background Induction of mutation through chemical mutagenesis is a novel approach for preparing diverse germplasm. Introduction of functional alleles in the starch biosynthetic genes help in the improvement of the quality and yield of cereals. Results In the present study, a set of 350 stable mutant lines were used to evaluate dynamic variation of the total starch contents. A megazyme kits were used for measuring the total starch content, resistant starch, amylose, and amylopectin content. Analysis of variance showed significant variation (p < 0.05) in starch content within the population. Furthermore, two high starch mutants (JE0173 and JE0218) and two low starch mutants (JE0089 and JE0418) were selected for studying different traits. A multiple comparison test showed that significant variation in all physiological and morphological traits, with respect to the parent variety (J411) in 2019–2020 and 2020–2021. The quantitative expression of starch metabolic genes revealed that eleven genes of JE0173 and twelve genes of JE0218 had consistent expression in high starch mutant lines. Similarly, in low starch mutant lines, eleven genes of JE0089 and thirteen genes of JE0418 had consistent expression in all stages of seed development. An additional two candidate genes showed over-expression (PHO1, PUL) in the high starch mutant lines, indicating that other starch metabolic genes may also contribute to the starch biosynthesis. The overexpression of SSII, SSIII and SBEI in JE0173 may be due to presence of missense mutations in these genes and SSI also showed overexpression which may be due to 3-primer_UTR variant. These mutations can affect the other starch related genes and help to increase the starch content in this mutant line (JE0173). Conclusions This study screened a large scale of mutant population and identified mutants, could provide useful genetic resources for the study of starch biosynthesis and genetic improvement of wheat in the future. Further study will help to understand new genes which are responsible for the fluctuation of total starch.https://doi.org/10.1186/s12870-023-04389-3Functional genesMutationQuality traitsStarchWheat |
| spellingShingle | Ahsan Irshad Huijun Guo Hongchun Xiong Yongdun Xie Hua Jin Jiayu Gu Chaojie Wang Liqun Yu Xianghui Wen Shirong Zhao Luxiang Liu Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat BMC Plant Biology Functional genes Mutation Quality traits Starch Wheat |
| title | Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat |
| title_full | Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat |
| title_fullStr | Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat |
| title_full_unstemmed | Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat |
| title_short | Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat |
| title_sort | evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat |
| topic | Functional genes Mutation Quality traits Starch Wheat |
| url | https://doi.org/10.1186/s12870-023-04389-3 |
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