Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breeds
<p>This study aimed to reveal the genetic variability of mitochondrial DNA (mtDNA) displacement-loop (D-loop) region in 62 animals belonging to three native Turkish cattle breeds, namely Anatolian Black (AB), East Anatolian Red (EAR) and Zavot (ZAV), and to conduct phylogenetic relationship an...
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2023-01-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/66/31/2023/aab-66-31-2023.pdf |
| _version_ | 1797944747523833856 |
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| author | E. Demir E. Demir N. Moravčíková B. Argun Karsli R. Kasarda I. Aytekin U. Bilginer T. Karsli |
| author_facet | E. Demir E. Demir N. Moravčíková B. Argun Karsli R. Kasarda I. Aytekin U. Bilginer T. Karsli |
| author_sort | E. Demir |
| collection | DOAJ |
| description | <p>This study aimed to reveal the genetic variability of
mitochondrial DNA (mtDNA) displacement-loop (D-loop) region in 62 animals
belonging to three native Turkish cattle breeds, namely Anatolian Black
(AB), East Anatolian Red (EAR) and Zavot (ZAV), and to conduct phylogenetic
relationship analyses to obtain deeper information on their genetic origin
and breeding history by comparison of 6 taurine and 11 indicine
breeds, together with 66 polymorphic sites, a total of 31 haplotypes, of
which 15, 10 and 6 were detected in AB, EAR and ZAV, respectively. Mean
nucleotide and haplotype diversity were 0.01 and 0.891, respectively,
whereas the genetic differentiation derived from Wright's <span class="inline-formula"><i>F</i><sub>ST</sub></span> index
was 0.174 across the breeds. A significant level of total variation
(17.42 %) was observed among breeds in molecular variance analysis. Six
main haplogroups (T, T1, T2, T3, Q and I2) were detected in Anatolian cattle
populations, where T3 was the most frequent among breeds (43.55 %),
whereas I2, an indicine specific haplogroup, was observed only in ZAV. At
the breed level, phylogenetic analyses supported by 198 sequences of 17
cattle breeds and 3 outgroup species retrieved from the GenBank clustered
native Turkish cattle breeds with the taurine group rather than the indicine
one, as expected. However, indicine admixture at low frequency (8.89 %)
was detected in the ZAV breed for the first time due to more likely gene
flow from indicine cattle breeds raised in neighbour countries, particularly
Iran. This finding should be further investigated in all native Turkish and
indicine cattle breeds from nearby countries to clarify gene flow and
indicine admixture in Anatolian cattle.</p> |
| first_indexed | 2024-04-10T20:44:33Z |
| format | Article |
| id | doaj.art-39a3eb2883de4808857016f6c81fa5f5 |
| institution | Directory Open Access Journal |
| issn | 0003-9438 2363-9822 |
| language | English |
| last_indexed | 2024-04-10T20:44:33Z |
| publishDate | 2023-01-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj.art-39a3eb2883de4808857016f6c81fa5f52023-01-24T09:25:09ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222023-01-0166314010.5194/aab-66-31-2023Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breedsE. Demir0E. Demir1N. Moravčíková2B. Argun Karsli3R. Kasarda4I. Aytekin5U. Bilginer6T. Karsli7Department of Animal Science, Faculty of Agriculture, Akdeniz University, Antalya, 07058, TürkiyeInstitute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra 94976, Slovak RepublicInstitute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra 94976, Slovak RepublicDepartment of Agricultural Biotechnology, Faculty of Agriculture, Eskisehir Osmangazi University, Eskisehir 26040, TürkiyeInstitute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra 94976, Slovak RepublicDepartment of Animal Science, Faculty of Agriculture, University of Selçuk, Konya 42130, TürkiyeDepartment of Animal Science, Faculty of Agriculture, Akdeniz University, Antalya, 07058, TürkiyeDepartment of Animal Science, Faculty of Agriculture, Eskisehir Osmangazi University, Eskisehir 26160, Türkiye<p>This study aimed to reveal the genetic variability of mitochondrial DNA (mtDNA) displacement-loop (D-loop) region in 62 animals belonging to three native Turkish cattle breeds, namely Anatolian Black (AB), East Anatolian Red (EAR) and Zavot (ZAV), and to conduct phylogenetic relationship analyses to obtain deeper information on their genetic origin and breeding history by comparison of 6 taurine and 11 indicine breeds, together with 66 polymorphic sites, a total of 31 haplotypes, of which 15, 10 and 6 were detected in AB, EAR and ZAV, respectively. Mean nucleotide and haplotype diversity were 0.01 and 0.891, respectively, whereas the genetic differentiation derived from Wright's <span class="inline-formula"><i>F</i><sub>ST</sub></span> index was 0.174 across the breeds. A significant level of total variation (17.42 %) was observed among breeds in molecular variance analysis. Six main haplogroups (T, T1, T2, T3, Q and I2) were detected in Anatolian cattle populations, where T3 was the most frequent among breeds (43.55 %), whereas I2, an indicine specific haplogroup, was observed only in ZAV. At the breed level, phylogenetic analyses supported by 198 sequences of 17 cattle breeds and 3 outgroup species retrieved from the GenBank clustered native Turkish cattle breeds with the taurine group rather than the indicine one, as expected. However, indicine admixture at low frequency (8.89 %) was detected in the ZAV breed for the first time due to more likely gene flow from indicine cattle breeds raised in neighbour countries, particularly Iran. This finding should be further investigated in all native Turkish and indicine cattle breeds from nearby countries to clarify gene flow and indicine admixture in Anatolian cattle.</p>https://aab.copernicus.org/articles/66/31/2023/aab-66-31-2023.pdf |
| spellingShingle | E. Demir E. Demir N. Moravčíková B. Argun Karsli R. Kasarda I. Aytekin U. Bilginer T. Karsli Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breeds Archives Animal Breeding |
| title | Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breeds |
| title_full | Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breeds |
| title_fullStr | Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breeds |
| title_full_unstemmed | Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breeds |
| title_short | Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breeds |
| title_sort | mitochondrial dna diversity of d loop region in three native turkish cattle breeds |
| url | https://aab.copernicus.org/articles/66/31/2023/aab-66-31-2023.pdf |
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