An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolators
Summary: The cost of supply cylinders can be expensive for smaller gnotobiotic facilities on strict budgets. Here, we present an alternative approach to enter supplies into germ-free isolators that does not require large cylinder drums (supply cylinders). We describe procedures for autoclaving doubl...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722003914 |
| _version_ | 1817978385553424384 |
|---|---|
| author | Samay Trec Mariah De Virgilio Chris Christou |
| author_facet | Samay Trec Mariah De Virgilio Chris Christou |
| author_sort | Samay Trec |
| collection | DOAJ |
| description | Summary: The cost of supply cylinders can be expensive for smaller gnotobiotic facilities on strict budgets. Here, we present an alternative approach to enter supplies into germ-free isolators that does not require large cylinder drums (supply cylinders). We describe procedures for autoclaving double-bagged packs of supplies, sealing the packs aseptically into zip lock bags in biosafety cabinet, and entering supply packages into isolators. Our gnotobiotic facility has been using this protocol since 2018 and has been effective in maintaining germ-free status. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T22:29:28Z |
| format | Article |
| id | doaj.art-39a4d3b8b2e8427e9a555978b8d623e3 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T22:29:28Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-39a4d3b8b2e8427e9a555978b8d623e32022-12-22T02:26:59ZengElsevierSTAR Protocols2666-16672022-09-0133101511An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolatorsSamay Trec0Mariah De Virgilio1Chris Christou2South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia; Preclinical, Research and Imaging Laboratories, Adelaide, SA 5086, Australia; Corresponding authorSouth Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia; Preclinical, Research and Imaging Laboratories, Adelaide, SA 5086, Australia; Corresponding authorSouth Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia; Preclinical, Research and Imaging Laboratories, Adelaide, SA 5086, Australia; Corresponding authorSummary: The cost of supply cylinders can be expensive for smaller gnotobiotic facilities on strict budgets. Here, we present an alternative approach to enter supplies into germ-free isolators that does not require large cylinder drums (supply cylinders). We describe procedures for autoclaving double-bagged packs of supplies, sealing the packs aseptically into zip lock bags in biosafety cabinet, and entering supply packages into isolators. Our gnotobiotic facility has been using this protocol since 2018 and has been effective in maintaining germ-free status. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003914MicrobiologyModel Organisms |
| spellingShingle | Samay Trec Mariah De Virgilio Chris Christou An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolators STAR Protocols Microbiology Model Organisms |
| title | An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolators |
| title_full | An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolators |
| title_fullStr | An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolators |
| title_full_unstemmed | An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolators |
| title_short | An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolators |
| title_sort | alternative to cylinder drums for entering supplies and maintaining germ free rodent isolators |
| topic | Microbiology Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722003914 |
| work_keys_str_mv | AT samaytrec analternativetocylinderdrumsforenteringsuppliesandmaintaininggermfreerodentisolators AT mariahdevirgilio analternativetocylinderdrumsforenteringsuppliesandmaintaininggermfreerodentisolators AT chrischristou analternativetocylinderdrumsforenteringsuppliesandmaintaininggermfreerodentisolators AT samaytrec alternativetocylinderdrumsforenteringsuppliesandmaintaininggermfreerodentisolators AT mariahdevirgilio alternativetocylinderdrumsforenteringsuppliesandmaintaininggermfreerodentisolators AT chrischristou alternativetocylinderdrumsforenteringsuppliesandmaintaininggermfreerodentisolators |