Preliminary Evidence for Domestication Effects on the Genetic Diversity of <i>Guazuma crinita</i> in the Peruvian Amazon
<i>Guazuma crinita,</i> a fast-growing timber tree species, was chosen for domestication in the Peruvian Amazon because it can be harvested at an early age and it contributes to the livelihood of local farmers. Although it is in an early stage of domestication, we do not know the impact...
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MDPI AG
2020-07-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/11/8/795 |
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| author | Lady Laura Tuisima-Coral Petra Hlásná Čepková John C. Weber Bohdan Lojka |
| author_facet | Lady Laura Tuisima-Coral Petra Hlásná Čepková John C. Weber Bohdan Lojka |
| author_sort | Lady Laura Tuisima-Coral |
| collection | DOAJ |
| description | <i>Guazuma crinita,</i> a fast-growing timber tree species, was chosen for domestication in the Peruvian Amazon because it can be harvested at an early age and it contributes to the livelihood of local farmers. Although it is in an early stage of domestication, we do not know the impact of the domestication process on its genetic resources. Amplified fragment length polymorphic (AFLP) fingerprints were used to estimate the genetic diversity of <i>G. crinita</i> populations in different stages of domestication. Our objectives were (i) to estimate the level of genetic diversity in <i>G. crinita</i> using AFLP markers, (ii) to describe how the genetic diversity is distributed within and among populations and provenances, and (iii) to assess the genetic diversity in naturally regenerated, cultivated and semi-domesticated populations. We generated fingerprints for 58 leaf samples representing eight provenances and the three population types. We used seven selective primer combinations. A total of 171 fragments were amplified with 99.4% polymorphism at the species level. Nei’s genetic diversity and Shannon information index were slightly higher in the naturally regenerated population than in the cultivated and semi-domesticated populations (<i>He</i> = 0.10, 0.09 and 0.09; <i>I</i> = 0.19, 0.15 and 0.16, respectively). The analysis of molecular variation showed higher genetic diversity within rather than among provenances (84% and 4%, respectively). Cluster analysis (unweighted pair group method with arithmetic mean) and principal coordinate analysis did not show correspondence between genetic and geographic distance. There was significant genetic differentiation among population types (F<sub>st</sub> <i>=</i> 0.12 at <i>p ˂</i> 0.001). The sample size was small, so the results are considered as preliminary, pending further research with larger sample sizes. Nevertheless, these results suggest that domestication has a slight but significant effect on the diversity levels of <i>G. crinita</i> and this should be considered when planning a domestication program. |
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| spelling | doaj.art-39a702daad534ed5a2c7074f90948a7e2023-11-20T07:40:55ZengMDPI AGForests1999-49072020-07-0111879510.3390/f11080795Preliminary Evidence for Domestication Effects on the Genetic Diversity of <i>Guazuma crinita</i> in the Peruvian AmazonLady Laura Tuisima-Coral0Petra Hlásná Čepková1John C. Weber2Bohdan Lojka3Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, 16500 Prague, Czech RepublicCrop Research Institute, Department of Gene Bank, Drnovska 507/73 Praha 6-Ruzyne, 16521 Prague, Czech RepublicWorld Agroforestry Centre, Lima 15074, PeruDepartment of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, 16500 Prague, Czech Republic<i>Guazuma crinita,</i> a fast-growing timber tree species, was chosen for domestication in the Peruvian Amazon because it can be harvested at an early age and it contributes to the livelihood of local farmers. Although it is in an early stage of domestication, we do not know the impact of the domestication process on its genetic resources. Amplified fragment length polymorphic (AFLP) fingerprints were used to estimate the genetic diversity of <i>G. crinita</i> populations in different stages of domestication. Our objectives were (i) to estimate the level of genetic diversity in <i>G. crinita</i> using AFLP markers, (ii) to describe how the genetic diversity is distributed within and among populations and provenances, and (iii) to assess the genetic diversity in naturally regenerated, cultivated and semi-domesticated populations. We generated fingerprints for 58 leaf samples representing eight provenances and the three population types. We used seven selective primer combinations. A total of 171 fragments were amplified with 99.4% polymorphism at the species level. Nei’s genetic diversity and Shannon information index were slightly higher in the naturally regenerated population than in the cultivated and semi-domesticated populations (<i>He</i> = 0.10, 0.09 and 0.09; <i>I</i> = 0.19, 0.15 and 0.16, respectively). The analysis of molecular variation showed higher genetic diversity within rather than among provenances (84% and 4%, respectively). Cluster analysis (unweighted pair group method with arithmetic mean) and principal coordinate analysis did not show correspondence between genetic and geographic distance. There was significant genetic differentiation among population types (F<sub>st</sub> <i>=</i> 0.12 at <i>p ˂</i> 0.001). The sample size was small, so the results are considered as preliminary, pending further research with larger sample sizes. Nevertheless, these results suggest that domestication has a slight but significant effect on the diversity levels of <i>G. crinita</i> and this should be considered when planning a domestication program.https://www.mdpi.com/1999-4907/11/8/795genetic diversitygenetic differentiationnatural regenerationcultivated populationsemi-domesticated population |
| spellingShingle | Lady Laura Tuisima-Coral Petra Hlásná Čepková John C. Weber Bohdan Lojka Preliminary Evidence for Domestication Effects on the Genetic Diversity of <i>Guazuma crinita</i> in the Peruvian Amazon Forests genetic diversity genetic differentiation natural regeneration cultivated population semi-domesticated population |
| title | Preliminary Evidence for Domestication Effects on the Genetic Diversity of <i>Guazuma crinita</i> in the Peruvian Amazon |
| title_full | Preliminary Evidence for Domestication Effects on the Genetic Diversity of <i>Guazuma crinita</i> in the Peruvian Amazon |
| title_fullStr | Preliminary Evidence for Domestication Effects on the Genetic Diversity of <i>Guazuma crinita</i> in the Peruvian Amazon |
| title_full_unstemmed | Preliminary Evidence for Domestication Effects on the Genetic Diversity of <i>Guazuma crinita</i> in the Peruvian Amazon |
| title_short | Preliminary Evidence for Domestication Effects on the Genetic Diversity of <i>Guazuma crinita</i> in the Peruvian Amazon |
| title_sort | preliminary evidence for domestication effects on the genetic diversity of i guazuma crinita i in the peruvian amazon |
| topic | genetic diversity genetic differentiation natural regeneration cultivated population semi-domesticated population |
| url | https://www.mdpi.com/1999-4907/11/8/795 |
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