New Core-Shell Nanostructures for FRET Studies: Synthesis, Characterization, and Quantitative Analysis
This work describes the synthesis and characterization of new core-shell material designed for Förster resonance energy transfer (FRET) studies. Synthesis, structural and optical properties of core-shell nanostructures with a large number of two kinds of fluorophores bound to the shell are presented...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/6/3182 |
| _version_ | 1797470881401798656 |
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| author | Anna Synak Elżbieta Adamska Leszek Kułak Beata Grobelna Paweł Niedziałkowski Piotr Bojarski |
| author_facet | Anna Synak Elżbieta Adamska Leszek Kułak Beata Grobelna Paweł Niedziałkowski Piotr Bojarski |
| author_sort | Anna Synak |
| collection | DOAJ |
| description | This work describes the synthesis and characterization of new core-shell material designed for Förster resonance energy transfer (FRET) studies. Synthesis, structural and optical properties of core-shell nanostructures with a large number of two kinds of fluorophores bound to the shell are presented. As fluorophores, strongly fluorescent rhodamine 101 and rhodamine 110 chloride were selected. The dyes exhibit significant spectral overlap between acceptor absorption and donor emission spectra, which enables effective FRET. Core-shell nanoparticles strongly differing in the ratio of donors to acceptor numbers were prepared. This leads to two different interesting cases: typical single-step FRET or multistep energy migration preceding FRET. The single-step FRET model that was designed and presented by some of us recently for core-shell nanoparticles is herein experimentally verified. Very good agreement between the analytical expression for donor fluorescence intensity decay and experimental data was obtained, which confirmed the correctness of the model. Multistep energy migration between donors preceding the final transfer to the acceptor can also be successfully described. In this case, however, experimental data are compared with the results of Monte Carlo simulations, as there is no respective analytical expression. Excellent agreement in this more general case evidences the usefulness of this numerical method in the design and prediction of the properties of the synthesized core-shell nanoparticles labelled with multiple and chemically different fluorophores. |
| first_indexed | 2024-03-09T19:42:12Z |
| format | Article |
| id | doaj.art-39a938887fc841cfa1380af9efe29ecb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:42:12Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-39a938887fc841cfa1380af9efe29ecb2023-11-24T01:34:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01236318210.3390/ijms23063182New Core-Shell Nanostructures for FRET Studies: Synthesis, Characterization, and Quantitative AnalysisAnna Synak0Elżbieta Adamska1Leszek Kułak2Beata Grobelna3Paweł Niedziałkowski4Piotr Bojarski5Faculty of Mathematics, Physics and Informatics, University of Gdansk, Wita Stwosza 57, 80-308 Gdansk, PolandFaculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, PolandFaculty of Technical Physics and Applied Mathematics, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, PolandFaculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, PolandFaculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, PolandFaculty of Mathematics, Physics and Informatics, University of Gdansk, Wita Stwosza 57, 80-308 Gdansk, PolandThis work describes the synthesis and characterization of new core-shell material designed for Förster resonance energy transfer (FRET) studies. Synthesis, structural and optical properties of core-shell nanostructures with a large number of two kinds of fluorophores bound to the shell are presented. As fluorophores, strongly fluorescent rhodamine 101 and rhodamine 110 chloride were selected. The dyes exhibit significant spectral overlap between acceptor absorption and donor emission spectra, which enables effective FRET. Core-shell nanoparticles strongly differing in the ratio of donors to acceptor numbers were prepared. This leads to two different interesting cases: typical single-step FRET or multistep energy migration preceding FRET. The single-step FRET model that was designed and presented by some of us recently for core-shell nanoparticles is herein experimentally verified. Very good agreement between the analytical expression for donor fluorescence intensity decay and experimental data was obtained, which confirmed the correctness of the model. Multistep energy migration between donors preceding the final transfer to the acceptor can also be successfully described. In this case, however, experimental data are compared with the results of Monte Carlo simulations, as there is no respective analytical expression. Excellent agreement in this more general case evidences the usefulness of this numerical method in the design and prediction of the properties of the synthesized core-shell nanoparticles labelled with multiple and chemically different fluorophores.https://www.mdpi.com/1422-0067/23/6/3182core-shell nanostructuresTiO<sub>2</sub>@SiO<sub>2</sub>FRETluminescent materials |
| spellingShingle | Anna Synak Elżbieta Adamska Leszek Kułak Beata Grobelna Paweł Niedziałkowski Piotr Bojarski New Core-Shell Nanostructures for FRET Studies: Synthesis, Characterization, and Quantitative Analysis International Journal of Molecular Sciences core-shell nanostructures TiO<sub>2</sub>@SiO<sub>2</sub> FRET luminescent materials |
| title | New Core-Shell Nanostructures for FRET Studies: Synthesis, Characterization, and Quantitative Analysis |
| title_full | New Core-Shell Nanostructures for FRET Studies: Synthesis, Characterization, and Quantitative Analysis |
| title_fullStr | New Core-Shell Nanostructures for FRET Studies: Synthesis, Characterization, and Quantitative Analysis |
| title_full_unstemmed | New Core-Shell Nanostructures for FRET Studies: Synthesis, Characterization, and Quantitative Analysis |
| title_short | New Core-Shell Nanostructures for FRET Studies: Synthesis, Characterization, and Quantitative Analysis |
| title_sort | new core shell nanostructures for fret studies synthesis characterization and quantitative analysis |
| topic | core-shell nanostructures TiO<sub>2</sub>@SiO<sub>2</sub> FRET luminescent materials |
| url | https://www.mdpi.com/1422-0067/23/6/3182 |
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