Obesity Correlates with Chronic Inflammation of the Innate Immune System in Preeclampsia and HELLP Syndrome during Pregnancy
HELLP syndrome is characterized by hemolysis, elevated liver enzymes, and a low platelet count and poses an increased risk to the pregnant woman and the unborn child. Individual risk factors such as obesity may alter immunocompetence and influence the course of preeclampsia (PE) or HELLP syndrome. B...
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MDPI AG
2023-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/10/2851 |
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| author | Julia Rimboeck Michael Gruber Marco Weigl Pia Huber Dirk Lunz Walter Petermichl |
| author_facet | Julia Rimboeck Michael Gruber Marco Weigl Pia Huber Dirk Lunz Walter Petermichl |
| author_sort | Julia Rimboeck |
| collection | DOAJ |
| description | HELLP syndrome is characterized by hemolysis, elevated liver enzymes, and a low platelet count and poses an increased risk to the pregnant woman and the unborn child. Individual risk factors such as obesity may alter immunocompetence and influence the course of preeclampsia (PE) or HELLP syndrome. Blood samples were collected from 21 pregnant women (7 healthy, 6 with PE, and 8 with HELLP syndrome) and polymorphonuclear neutrophils (PMNs) were subsequently isolated. Production of radical oxygen species (ROS), cell movement, and NETosis were assessed by live-cell imaging. Surface protein expression and oxidative burst were analyzed by flow cytometry. PE and HELLP patients had significantly higher BMI compared to the healthy control group. Depending on the expression of CD11b, CD62L, and CD66b on PMNs, a surface protein activation sum scale (SPASS) was calculated. PMNs from patients with high SPASS values showed prolonged and more targeted migration with delayed ROS production and NETosis. Obesity is associated with a chronic inflammatory state, which in combination with immunological triggers during pregnancy could modulate PMN functions. Pregnant women with higher BMI tend to have higher SPASS values, indicating activation of the innate immune system that could co-trigger PE or HELLP syndrome. |
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| id | doaj.art-39a99e3bd0d64b69a66214ca2e8b53b8 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T21:24:16Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-39a99e3bd0d64b69a66214ca2e8b53b82023-11-19T15:48:01ZengMDPI AGBiomedicines2227-90592023-10-011110285110.3390/biomedicines11102851Obesity Correlates with Chronic Inflammation of the Innate Immune System in Preeclampsia and HELLP Syndrome during PregnancyJulia Rimboeck0Michael Gruber1Marco Weigl2Pia Huber3Dirk Lunz4Walter Petermichl5Department of Anesthesiology, University Hospital of Regensburg, 93042 Regensburg, GermanyDepartment of Anesthesiology, University Hospital of Regensburg, 93042 Regensburg, GermanyUniversity Department of Obstetrics and Gynecology at the Hospital St. Hedwig of the Order of St. John, University of Regensburg, 93049 Regensburg, GermanyDepartment of Anesthesiology, University Hospital of Regensburg, 93042 Regensburg, GermanyDepartment of Anesthesiology, University Hospital of Regensburg, 93042 Regensburg, GermanyDepartment of Anesthesiology, University Hospital of Regensburg, 93042 Regensburg, GermanyHELLP syndrome is characterized by hemolysis, elevated liver enzymes, and a low platelet count and poses an increased risk to the pregnant woman and the unborn child. Individual risk factors such as obesity may alter immunocompetence and influence the course of preeclampsia (PE) or HELLP syndrome. Blood samples were collected from 21 pregnant women (7 healthy, 6 with PE, and 8 with HELLP syndrome) and polymorphonuclear neutrophils (PMNs) were subsequently isolated. Production of radical oxygen species (ROS), cell movement, and NETosis were assessed by live-cell imaging. Surface protein expression and oxidative burst were analyzed by flow cytometry. PE and HELLP patients had significantly higher BMI compared to the healthy control group. Depending on the expression of CD11b, CD62L, and CD66b on PMNs, a surface protein activation sum scale (SPASS) was calculated. PMNs from patients with high SPASS values showed prolonged and more targeted migration with delayed ROS production and NETosis. Obesity is associated with a chronic inflammatory state, which in combination with immunological triggers during pregnancy could modulate PMN functions. Pregnant women with higher BMI tend to have higher SPASS values, indicating activation of the innate immune system that could co-trigger PE or HELLP syndrome.https://www.mdpi.com/2227-9059/11/10/2851HELLPpreeclampsiaHDPpregnancyobesityPMNs |
| spellingShingle | Julia Rimboeck Michael Gruber Marco Weigl Pia Huber Dirk Lunz Walter Petermichl Obesity Correlates with Chronic Inflammation of the Innate Immune System in Preeclampsia and HELLP Syndrome during Pregnancy Biomedicines HELLP preeclampsia HDP pregnancy obesity PMNs |
| title | Obesity Correlates with Chronic Inflammation of the Innate Immune System in Preeclampsia and HELLP Syndrome during Pregnancy |
| title_full | Obesity Correlates with Chronic Inflammation of the Innate Immune System in Preeclampsia and HELLP Syndrome during Pregnancy |
| title_fullStr | Obesity Correlates with Chronic Inflammation of the Innate Immune System in Preeclampsia and HELLP Syndrome during Pregnancy |
| title_full_unstemmed | Obesity Correlates with Chronic Inflammation of the Innate Immune System in Preeclampsia and HELLP Syndrome during Pregnancy |
| title_short | Obesity Correlates with Chronic Inflammation of the Innate Immune System in Preeclampsia and HELLP Syndrome during Pregnancy |
| title_sort | obesity correlates with chronic inflammation of the innate immune system in preeclampsia and hellp syndrome during pregnancy |
| topic | HELLP preeclampsia HDP pregnancy obesity PMNs |
| url | https://www.mdpi.com/2227-9059/11/10/2851 |
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