Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion
Equine herpesvirus 1 (EHV-1) is an Alphaherpesvirus infecting not only horses but also other equid and non-equid mammals. It can cause respiratory distress, stillbirth and neonatal death, abortion, and neurological disease. The different forms of disease induced by EHV-1 infection can have dramatic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/10/916 |
| _version_ | 1817968794504527872 |
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| author | Gabrielle Sutton Marie Garvey Ann Cullinane Marion Jourdan Christine Fortier Peggy Moreau Marc Foursin Annick Gryspeerdt Virginie Maisonnier Christel Marcillaud-Pitel Loïc Legrand Romain Paillot Stéphane Pronost |
| author_facet | Gabrielle Sutton Marie Garvey Ann Cullinane Marion Jourdan Christine Fortier Peggy Moreau Marc Foursin Annick Gryspeerdt Virginie Maisonnier Christel Marcillaud-Pitel Loïc Legrand Romain Paillot Stéphane Pronost |
| author_sort | Gabrielle Sutton |
| collection | DOAJ |
| description | Equine herpesvirus 1 (EHV-1) is an Alphaherpesvirus infecting not only horses but also other equid and non-equid mammals. It can cause respiratory distress, stillbirth and neonatal death, abortion, and neurological disease. The different forms of disease induced by EHV-1 infection can have dramatic consequences on the equine industry, and thus the virus represents a great challenge for the equine and scientific community. This report describes the progress of a major EHV-1 outbreak that took place in Normandy in 2009, during which the three forms of disease were observed. A collection of EHV-1 strains isolated in France and Belgium from 2012 to 2018 were subsequently genetically analysed in order to characterise EHV-1 strain circulation. The open reading frame 30 (ORF30) non-neuropathogenic associated mutation A<sub>2254</sub> was the most represented among 148 samples analysed in this study. ORF30 was also sequenced for 14 strains and compared to previously published sequences. Finally, a more global phylogenetic approach was performed based on a recently described Multilocus Sequence Typing (MLST) method. French and Belgian strains were clustered with known strains isolated in United Kingdom and Ireland, with no correlation between the phylogeny and the time of collection or location. This new MLST approach could be a tool to help understand epidemics in stud farms. |
| first_indexed | 2024-04-13T20:13:34Z |
| format | Article |
| id | doaj.art-39acbe0a29ae477d83b8ed3c4d011184 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-13T20:13:34Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-39acbe0a29ae477d83b8ed3c4d0111842022-12-22T02:31:46ZengMDPI AGViruses1999-49152019-10-01111091610.3390/v11100916v11100916Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and AbortionGabrielle Sutton0Marie Garvey1Ann Cullinane2Marion Jourdan3Christine Fortier4Peggy Moreau5Marc Foursin6Annick Gryspeerdt7Virginie Maisonnier8Christel Marcillaud-Pitel9Loïc Legrand10Romain Paillot11Stéphane Pronost12LABÉO Frank Duncombe, 14280 Saint-Contest, FranceIrish Equine Centre, Johnstown, Naas, County Kildare, Eircode: W91 RH93, IrelandIrish Equine Centre, Johnstown, Naas, County Kildare, Eircode: W91 RH93, IrelandRESPE, 14280 Saint-Contest, FranceLABÉO Frank Duncombe, 14280 Saint-Contest, FranceClinique équine de la Boisrie, 61500 Chailloué, FranceClinique équine de la Boisrie, 61500 Chailloué, FranceEqui Focus Point Belgium, 8900 Ypres, BelgiumRESPE, 14280 Saint-Contest, FranceRESPE, 14280 Saint-Contest, FranceLABÉO Frank Duncombe, 14280 Saint-Contest, FranceLABÉO Frank Duncombe, 14280 Saint-Contest, FranceLABÉO Frank Duncombe, 14280 Saint-Contest, FranceEquine herpesvirus 1 (EHV-1) is an Alphaherpesvirus infecting not only horses but also other equid and non-equid mammals. It can cause respiratory distress, stillbirth and neonatal death, abortion, and neurological disease. The different forms of disease induced by EHV-1 infection can have dramatic consequences on the equine industry, and thus the virus represents a great challenge for the equine and scientific community. This report describes the progress of a major EHV-1 outbreak that took place in Normandy in 2009, during which the three forms of disease were observed. A collection of EHV-1 strains isolated in France and Belgium from 2012 to 2018 were subsequently genetically analysed in order to characterise EHV-1 strain circulation. The open reading frame 30 (ORF30) non-neuropathogenic associated mutation A<sub>2254</sub> was the most represented among 148 samples analysed in this study. ORF30 was also sequenced for 14 strains and compared to previously published sequences. Finally, a more global phylogenetic approach was performed based on a recently described Multilocus Sequence Typing (MLST) method. French and Belgian strains were clustered with known strains isolated in United Kingdom and Ireland, with no correlation between the phylogeny and the time of collection or location. This new MLST approach could be a tool to help understand epidemics in stud farms.https://www.mdpi.com/1999-4915/11/10/916equine herpesvirus type 1outbreakrespiratory diseaseabortionneuropathogenic strainmyeloencephalopathyphylogenyorf30mlst |
| spellingShingle | Gabrielle Sutton Marie Garvey Ann Cullinane Marion Jourdan Christine Fortier Peggy Moreau Marc Foursin Annick Gryspeerdt Virginie Maisonnier Christel Marcillaud-Pitel Loïc Legrand Romain Paillot Stéphane Pronost Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion Viruses equine herpesvirus type 1 outbreak respiratory disease abortion neuropathogenic strain myeloencephalopathy phylogeny orf30 mlst |
| title | Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion |
| title_full | Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion |
| title_fullStr | Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion |
| title_full_unstemmed | Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion |
| title_short | Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion |
| title_sort | molecular surveillance of ehv 1 strains circulating in france during and after the major 2009 outbreak in normandy involving respiratory infection neurological disorder and abortion |
| topic | equine herpesvirus type 1 outbreak respiratory disease abortion neuropathogenic strain myeloencephalopathy phylogeny orf30 mlst |
| url | https://www.mdpi.com/1999-4915/11/10/916 |
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