Molecular Characterization and Phylogenetic Analysis of <i>Spirometra</i> Tapeworms from Snakes in Hunan Province
Sparganosis is a neglected zoonotic parasitic disease that poses huge threats to humans worldwide. Snakes play an important role in sparganosis transmission because they are the most common second intermediate hosts for <i>Spirometra</i> parasites. However, the population genetics of <...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/2/62 |
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| author | Shu-Yu Chen Teng-Fang Gong Jun-Lin He Fen Li Wen-Chao Li Li-Xing Xie Xin-Rui Xie Yi-Song Liu Ying-Fang Zhou Wei Liu |
| author_facet | Shu-Yu Chen Teng-Fang Gong Jun-Lin He Fen Li Wen-Chao Li Li-Xing Xie Xin-Rui Xie Yi-Song Liu Ying-Fang Zhou Wei Liu |
| author_sort | Shu-Yu Chen |
| collection | DOAJ |
| description | Sparganosis is a neglected zoonotic parasitic disease that poses huge threats to humans worldwide. Snakes play an important role in sparganosis transmission because they are the most common second intermediate hosts for <i>Spirometra</i> parasites. However, the population genetics of <i>Spirometra</i> isolates from snakes is currently not well studied in China. The present study was performed to explore the molecular characteristics and phylogenetic analysis of <i>Spirometra</i> tapeworms from different species of snakes in Hunan Province. This study obtained 49 <i>Spirometra</i> isolates from 15 geographical areas in Hunan Province, Central China. Subsequently, the 18S and 28S ribosomal DNA (rDNA) fragments were amplified from the isolated parasites, and their sequences were analyzed to assess their genetic diversity. Phylogenetic analyses were performed using the maximum likelihood algorithm. The results showed that sequence variations among these isolates were 0–2.3% and 0–0.1% for 18S and 28S rDNA, respectively. The phylogenetic analysis showed that all <i>Spirometra</i> isolates from Hunan Province were clustered into the same branch with <i>Spirometra erinaceieuropaei</i> isolated from other areas (China, Vietnam, Australia). Moreover, the phylogenetic trees revealed that <i>Spirometra</i> is closely related to <i>Adenocephalus</i>, <i>Pyramicocephalus</i>, <i>Ligula</i>, <i>Dibothriocephalus</i>, <i>Schistocephalus</i>, and <i>Diphyllobothrium</i>. The <i>Spirometra</i> isolates of different hosts/regions in Hunan Province are not host segregated or geographically isolated, and support for the taxonomic status of <i>Spirometra</i> tapeworms in China has been added. These results provide reference values for future accurate identification and taxonomic status of <i>Spirometra</i> tapeworms in China. |
| first_indexed | 2024-03-09T20:53:21Z |
| format | Article |
| id | doaj.art-39acbf70349c474f83ef63cb16949ed0 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T20:53:21Z |
| publishDate | 2022-02-01 |
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| series | Veterinary Sciences |
| spelling | doaj.art-39acbf70349c474f83ef63cb16949ed02023-11-23T22:28:02ZengMDPI AGVeterinary Sciences2306-73812022-02-01926210.3390/vetsci9020062Molecular Characterization and Phylogenetic Analysis of <i>Spirometra</i> Tapeworms from Snakes in Hunan ProvinceShu-Yu Chen0Teng-Fang Gong1Jun-Lin He2Fen Li3Wen-Chao Li4Li-Xing Xie5Xin-Rui Xie6Yi-Song Liu7Ying-Fang Zhou8Wei Liu9Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaResearch Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaResearch Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaResearch Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaResearch Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaOrient Science & Technology College, Hunan Agriculture University, Changsha 410128, ChinaResearch Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaResearch Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaOrient Science & Technology College, Hunan Agriculture University, Changsha 410128, ChinaResearch Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaSparganosis is a neglected zoonotic parasitic disease that poses huge threats to humans worldwide. Snakes play an important role in sparganosis transmission because they are the most common second intermediate hosts for <i>Spirometra</i> parasites. However, the population genetics of <i>Spirometra</i> isolates from snakes is currently not well studied in China. The present study was performed to explore the molecular characteristics and phylogenetic analysis of <i>Spirometra</i> tapeworms from different species of snakes in Hunan Province. This study obtained 49 <i>Spirometra</i> isolates from 15 geographical areas in Hunan Province, Central China. Subsequently, the 18S and 28S ribosomal DNA (rDNA) fragments were amplified from the isolated parasites, and their sequences were analyzed to assess their genetic diversity. Phylogenetic analyses were performed using the maximum likelihood algorithm. The results showed that sequence variations among these isolates were 0–2.3% and 0–0.1% for 18S and 28S rDNA, respectively. The phylogenetic analysis showed that all <i>Spirometra</i> isolates from Hunan Province were clustered into the same branch with <i>Spirometra erinaceieuropaei</i> isolated from other areas (China, Vietnam, Australia). Moreover, the phylogenetic trees revealed that <i>Spirometra</i> is closely related to <i>Adenocephalus</i>, <i>Pyramicocephalus</i>, <i>Ligula</i>, <i>Dibothriocephalus</i>, <i>Schistocephalus</i>, and <i>Diphyllobothrium</i>. The <i>Spirometra</i> isolates of different hosts/regions in Hunan Province are not host segregated or geographically isolated, and support for the taxonomic status of <i>Spirometra</i> tapeworms in China has been added. These results provide reference values for future accurate identification and taxonomic status of <i>Spirometra</i> tapeworms in China.https://www.mdpi.com/2306-7381/9/2/62genetic variationphylogenetic analysisribosomal DNA<i>Spirometra erinaceieuropaei</i> |
| spellingShingle | Shu-Yu Chen Teng-Fang Gong Jun-Lin He Fen Li Wen-Chao Li Li-Xing Xie Xin-Rui Xie Yi-Song Liu Ying-Fang Zhou Wei Liu Molecular Characterization and Phylogenetic Analysis of <i>Spirometra</i> Tapeworms from Snakes in Hunan Province Veterinary Sciences genetic variation phylogenetic analysis ribosomal DNA <i>Spirometra erinaceieuropaei</i> |
| title | Molecular Characterization and Phylogenetic Analysis of <i>Spirometra</i> Tapeworms from Snakes in Hunan Province |
| title_full | Molecular Characterization and Phylogenetic Analysis of <i>Spirometra</i> Tapeworms from Snakes in Hunan Province |
| title_fullStr | Molecular Characterization and Phylogenetic Analysis of <i>Spirometra</i> Tapeworms from Snakes in Hunan Province |
| title_full_unstemmed | Molecular Characterization and Phylogenetic Analysis of <i>Spirometra</i> Tapeworms from Snakes in Hunan Province |
| title_short | Molecular Characterization and Phylogenetic Analysis of <i>Spirometra</i> Tapeworms from Snakes in Hunan Province |
| title_sort | molecular characterization and phylogenetic analysis of i spirometra i tapeworms from snakes in hunan province |
| topic | genetic variation phylogenetic analysis ribosomal DNA <i>Spirometra erinaceieuropaei</i> |
| url | https://www.mdpi.com/2306-7381/9/2/62 |
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