Small ubiquitin-related modifier 2 affects the intracellular survival of 2308 by regulating activation of the NF-κB pathway
Brucella is a genus of Gram-negative intracellular pathogens that cause animal and human diseases. Brucella survival and replication inside immune cells is critical for the establishment of chronic infections. Protein modifications by small ubiquitin-related modifier proteins and the NF-κB pathway a...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2021-01-01
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| Series: | Innate Immunity |
| Online Access: | https://doi.org/10.1177/1753425920972171 |
| _version_ | 1819056499514671104 |
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| author | Yueli Wang Jing Xi Peng Wu Huan Zhang Xiaoyu Deng Yong Wang Zhongchen Ma Jihai Yi Chuangfu Chen |
| author_facet | Yueli Wang Jing Xi Peng Wu Huan Zhang Xiaoyu Deng Yong Wang Zhongchen Ma Jihai Yi Chuangfu Chen |
| author_sort | Yueli Wang |
| collection | DOAJ |
| description | Brucella is a genus of Gram-negative intracellular pathogens that cause animal and human diseases. Brucella survival and replication inside immune cells is critical for the establishment of chronic infections. Protein modifications by small ubiquitin-related modifier proteins and the NF-κB pathway are involved in many cellular activities, playing major roles in regulating protein function that is essential for pathogenic bacteria during infection. However, the relationship between them in the intracellular survival of Brucella is still largely unknown. We demonstrated that Brucella abortus 2308 infection can activate the expression of small ubiquitin-related modifier-2 proteins in a time-dependent manner. We found the production of Th1 cytokines (IFN-γ and TNF-α) and the transcription of NF-κB/p65 were promoted by overexpression and inhibited by interference of small ubiquitin-related modifier-2. In addition, we showed that small ubiquitin-related modifier-2 can inhibit intracellular survival of Brucella abortus 2308 by regulating activation of the NF-κB pathway. Taken together, this work shows that small ubiquitin-related modifier-2 modification of NF-κB2/p65 is essential for the survival of Brucella abortus 2308 inside macrophages. This work may help to unravel the pathogenic mechanisms of Brucella infections. |
| first_indexed | 2024-12-21T13:24:23Z |
| format | Article |
| id | doaj.art-39adc9aae1224975b39804c8124ccb0c |
| institution | Directory Open Access Journal |
| issn | 1753-4259 1753-4267 |
| language | English |
| last_indexed | 2024-12-21T13:24:23Z |
| publishDate | 2021-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Innate Immunity |
| spelling | doaj.art-39adc9aae1224975b39804c8124ccb0c2022-12-21T19:02:29ZengSAGE PublishingInnate Immunity1753-42591753-42672021-01-012710.1177/1753425920972171Small ubiquitin-related modifier 2 affects the intracellular survival of 2308 by regulating activation of the NF-κB pathwayYueli WangJing XiPeng WuHuan ZhangXiaoyu DengYong WangZhongchen MaJihai YiChuangfu ChenBrucella is a genus of Gram-negative intracellular pathogens that cause animal and human diseases. Brucella survival and replication inside immune cells is critical for the establishment of chronic infections. Protein modifications by small ubiquitin-related modifier proteins and the NF-κB pathway are involved in many cellular activities, playing major roles in regulating protein function that is essential for pathogenic bacteria during infection. However, the relationship between them in the intracellular survival of Brucella is still largely unknown. We demonstrated that Brucella abortus 2308 infection can activate the expression of small ubiquitin-related modifier-2 proteins in a time-dependent manner. We found the production of Th1 cytokines (IFN-γ and TNF-α) and the transcription of NF-κB/p65 were promoted by overexpression and inhibited by interference of small ubiquitin-related modifier-2. In addition, we showed that small ubiquitin-related modifier-2 can inhibit intracellular survival of Brucella abortus 2308 by regulating activation of the NF-κB pathway. Taken together, this work shows that small ubiquitin-related modifier-2 modification of NF-κB2/p65 is essential for the survival of Brucella abortus 2308 inside macrophages. This work may help to unravel the pathogenic mechanisms of Brucella infections.https://doi.org/10.1177/1753425920972171 |
| spellingShingle | Yueli Wang Jing Xi Peng Wu Huan Zhang Xiaoyu Deng Yong Wang Zhongchen Ma Jihai Yi Chuangfu Chen Small ubiquitin-related modifier 2 affects the intracellular survival of 2308 by regulating activation of the NF-κB pathway Innate Immunity |
| title | Small ubiquitin-related modifier 2 affects the intracellular survival of 2308 by regulating activation of the NF-κB pathway |
| title_full | Small ubiquitin-related modifier 2 affects the intracellular survival of 2308 by regulating activation of the NF-κB pathway |
| title_fullStr | Small ubiquitin-related modifier 2 affects the intracellular survival of 2308 by regulating activation of the NF-κB pathway |
| title_full_unstemmed | Small ubiquitin-related modifier 2 affects the intracellular survival of 2308 by regulating activation of the NF-κB pathway |
| title_short | Small ubiquitin-related modifier 2 affects the intracellular survival of 2308 by regulating activation of the NF-κB pathway |
| title_sort | small ubiquitin related modifier 2 affects the intracellular survival of 2308 by regulating activation of the nf κb pathway |
| url | https://doi.org/10.1177/1753425920972171 |
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