Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and Vildagliptin
This paper sets out to determine whether silver nanoparticles conjugation enhance the antibacterial efficacy of clinically approved drugs. Silver conjugated Cephradine and Vildagliptin were synthesized and thoroughly characterized by ultraviolet visible spectrophotometry (UV-vis), Fourier transform...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-11-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/7/4/100 |
| _version_ | 1819128408146182144 |
|---|---|
| author | Abdulkader Masri Ayaz Anwar Dania Ahmed Ruqaiyyah Bano Siddiqui Muhammad Raza Shah Naveed Ahmed Khan |
| author_facet | Abdulkader Masri Ayaz Anwar Dania Ahmed Ruqaiyyah Bano Siddiqui Muhammad Raza Shah Naveed Ahmed Khan |
| author_sort | Abdulkader Masri |
| collection | DOAJ |
| description | This paper sets out to determine whether silver nanoparticles conjugation enhance the antibacterial efficacy of clinically approved drugs. Silver conjugated Cephradine and Vildagliptin were synthesized and thoroughly characterized by ultraviolet visible spectrophotometry (UV-vis), Fourier transform infrared (FT-IR) spectroscopic methods, atomic force microscopy (AFM), and dynamic light scattering (DLS) analysis. Using antibacterial assays, the effects of drugs alone and drugs-conjugated with silver nanoparticles were tested against a variety of Gram-negative and Gram-positive bacteria including neuropathogenic <i>Escherichia coli</i> K1, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), <i>Bacillus cereus</i> and <i>Streptococcus pyogenes</i>. Cytopathogenicity assays were performed to determine whether pretreatment of bacteria with drugs inhibit bacterial-mediated host cell cytotoxicity. The UV-vis spectra of both silver-drug nanoconjugates showed a characteristic surface plasmon resonance band in the range of 400⁻450 nm. AFM further confirmed the morphology of nanoparticles and revealed the formation of spherical nanoparticles with size distribution of 30⁻80 nm. FT-IR analysis demonstrated the involvement of Hydroxyl groups in both drugs in the stabilization of silver nanoparticles. Antibacterial assays showed that silver nanoparticle conjugation enhanced antibacterial potential of both Cephradine and Vildagliptin compared to the drugs alone. Pretreatment of bacteria with drugs inhibited <i>E. coli</i> K1-mediated host cell cytotoxicity. In summary, conjugation with silver nanoparticle enhanced antibacterial effects of clinically approved Cephradine. These findings suggest that modifying and/or repurposing clinically approved drugs using nanotechnology is a feasible approach in our search for effective antibacterial molecules. |
| first_indexed | 2024-12-22T08:27:21Z |
| format | Article |
| id | doaj.art-39aece6cbc524b7fa8cb45548147554c |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-22T08:27:21Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-39aece6cbc524b7fa8cb45548147554c2022-12-21T18:32:36ZengMDPI AGAntibiotics2079-63822018-11-017410010.3390/antibiotics7040100antibiotics7040100Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and VildagliptinAbdulkader Masri0Ayaz Anwar1Dania Ahmed2Ruqaiyyah Bano Siddiqui3Muhammad Raza Shah4Naveed Ahmed Khan5Department of Biological Sciences, School of Science and Technology, Sunway University, Bandar Sunway 47500, MalaysiaDepartment of Biological Sciences, School of Science and Technology, Sunway University, Bandar Sunway 47500, MalaysiaHEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 74600, PakistanDepartment of Biological Sciences, School of Science and Technology, Sunway University, Bandar Sunway 47500, MalaysiaHEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 74600, PakistanDepartment of Biological Sciences, School of Science and Technology, Sunway University, Bandar Sunway 47500, MalaysiaThis paper sets out to determine whether silver nanoparticles conjugation enhance the antibacterial efficacy of clinically approved drugs. Silver conjugated Cephradine and Vildagliptin were synthesized and thoroughly characterized by ultraviolet visible spectrophotometry (UV-vis), Fourier transform infrared (FT-IR) spectroscopic methods, atomic force microscopy (AFM), and dynamic light scattering (DLS) analysis. Using antibacterial assays, the effects of drugs alone and drugs-conjugated with silver nanoparticles were tested against a variety of Gram-negative and Gram-positive bacteria including neuropathogenic <i>Escherichia coli</i> K1, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), <i>Bacillus cereus</i> and <i>Streptococcus pyogenes</i>. Cytopathogenicity assays were performed to determine whether pretreatment of bacteria with drugs inhibit bacterial-mediated host cell cytotoxicity. The UV-vis spectra of both silver-drug nanoconjugates showed a characteristic surface plasmon resonance band in the range of 400⁻450 nm. AFM further confirmed the morphology of nanoparticles and revealed the formation of spherical nanoparticles with size distribution of 30⁻80 nm. FT-IR analysis demonstrated the involvement of Hydroxyl groups in both drugs in the stabilization of silver nanoparticles. Antibacterial assays showed that silver nanoparticle conjugation enhanced antibacterial potential of both Cephradine and Vildagliptin compared to the drugs alone. Pretreatment of bacteria with drugs inhibited <i>E. coli</i> K1-mediated host cell cytotoxicity. In summary, conjugation with silver nanoparticle enhanced antibacterial effects of clinically approved Cephradine. These findings suggest that modifying and/or repurposing clinically approved drugs using nanotechnology is a feasible approach in our search for effective antibacterial molecules.https://www.mdpi.com/2079-6382/7/4/100CephradineVildagliptinnanoparticlesantibacterialnanotechnology |
| spellingShingle | Abdulkader Masri Ayaz Anwar Dania Ahmed Ruqaiyyah Bano Siddiqui Muhammad Raza Shah Naveed Ahmed Khan Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and Vildagliptin Antibiotics Cephradine Vildagliptin nanoparticles antibacterial nanotechnology |
| title | Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and Vildagliptin |
| title_full | Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and Vildagliptin |
| title_fullStr | Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and Vildagliptin |
| title_full_unstemmed | Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and Vildagliptin |
| title_short | Silver Nanoparticle Conjugation-Enhanced Antibacterial Efficacy of Clinically Approved Drugs Cephradine and Vildagliptin |
| title_sort | silver nanoparticle conjugation enhanced antibacterial efficacy of clinically approved drugs cephradine and vildagliptin |
| topic | Cephradine Vildagliptin nanoparticles antibacterial nanotechnology |
| url | https://www.mdpi.com/2079-6382/7/4/100 |
| work_keys_str_mv | AT abdulkadermasri silvernanoparticleconjugationenhancedantibacterialefficacyofclinicallyapproveddrugscephradineandvildagliptin AT ayazanwar silvernanoparticleconjugationenhancedantibacterialefficacyofclinicallyapproveddrugscephradineandvildagliptin AT daniaahmed silvernanoparticleconjugationenhancedantibacterialefficacyofclinicallyapproveddrugscephradineandvildagliptin AT ruqaiyyahbanosiddiqui silvernanoparticleconjugationenhancedantibacterialefficacyofclinicallyapproveddrugscephradineandvildagliptin AT muhammadrazashah silvernanoparticleconjugationenhancedantibacterialefficacyofclinicallyapproveddrugscephradineandvildagliptin AT naveedahmedkhan silvernanoparticleconjugationenhancedantibacterialefficacyofclinicallyapproveddrugscephradineandvildagliptin |