Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm
Rhynchosporium is one of the main biotic stresses on barley production worldwide. A set of 312 spring barley accessions was tested in four different locations over 3 years, to identify novel genetic resistances to rhynchosporium and to explore the allelic diversity for resistance genes present in th...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/4/782 |
| _version_ | 1797437288250081280 |
|---|---|
| author | Jean-Noël Thauvin Joanne Russell Dominique Vequaud Mark Looseley Micha Bayer Pierre-Marie Le Roux Pierre Pin Robbie Waugh Anna Avrova |
| author_facet | Jean-Noël Thauvin Joanne Russell Dominique Vequaud Mark Looseley Micha Bayer Pierre-Marie Le Roux Pierre Pin Robbie Waugh Anna Avrova |
| author_sort | Jean-Noël Thauvin |
| collection | DOAJ |
| description | Rhynchosporium is one of the main biotic stresses on barley production worldwide. A set of 312 spring barley accessions was tested in four different locations over 3 years, to identify novel genetic resistances to rhynchosporium and to explore the allelic diversity for resistance genes present in this global germplasm collection. High-density genotypes from exome capture and RNA-seq were used to conduct high-resolution association mapping. Seven quantitative trait loci (QTL) were detected, including one in the <i>Rrs2</i> region, amongst five containing known resistances. Relatively short physical intervals harbouring these resistances were proposed, providing a platform for the identification of underlying genes and tightly linked genetic markers for use in marker assisted selection. Genes encoding kinases were present in four of the QTL, in addition to <i>Rrs1</i> and <i>Rrs18</i>, two loci known to contribute to rhynchosporium resistance. The frequencies and distributions of these novel and known QTL were superimposed on the regional origin of the landrace genotypes comprising the genome-wide association studies (GWAS) panel, highlighting the value of genetic resources as a source of diverse genetically controlled resistance to rhynchosporium. The detected QTL along with their linked genetic markers, could be exploited either directly for breeding purposes or for candidate gene identification in future studies. |
| first_indexed | 2024-03-09T11:18:00Z |
| format | Article |
| id | doaj.art-39b0dc5df8964e7588df9401223d9efa |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T11:18:00Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-39b0dc5df8964e7588df9401223d9efa2023-12-01T00:25:48ZengMDPI AGAgronomy2073-43952022-03-0112478210.3390/agronomy12040782Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley GermplasmJean-Noël Thauvin0Joanne Russell1Dominique Vequaud2Mark Looseley3Micha Bayer4Pierre-Marie Le Roux5Pierre Pin6Robbie Waugh7Anna Avrova8The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UKThe James Hutton Institute, Invergowrie, Dundee DD2 5DA, UKSecobra Recherches SAS, Centre de Bois-Henry, 78580 Maule, FranceThe James Hutton Institute, Invergowrie, Dundee DD2 5DA, UKThe James Hutton Institute, Invergowrie, Dundee DD2 5DA, UKSecobra Recherches SAS, Centre de Bois-Henry, 78580 Maule, FranceSecobra Recherches SAS, Centre de Bois-Henry, 78580 Maule, FranceThe James Hutton Institute, Invergowrie, Dundee DD2 5DA, UKThe James Hutton Institute, Invergowrie, Dundee DD2 5DA, UKRhynchosporium is one of the main biotic stresses on barley production worldwide. A set of 312 spring barley accessions was tested in four different locations over 3 years, to identify novel genetic resistances to rhynchosporium and to explore the allelic diversity for resistance genes present in this global germplasm collection. High-density genotypes from exome capture and RNA-seq were used to conduct high-resolution association mapping. Seven quantitative trait loci (QTL) were detected, including one in the <i>Rrs2</i> region, amongst five containing known resistances. Relatively short physical intervals harbouring these resistances were proposed, providing a platform for the identification of underlying genes and tightly linked genetic markers for use in marker assisted selection. Genes encoding kinases were present in four of the QTL, in addition to <i>Rrs1</i> and <i>Rrs18</i>, two loci known to contribute to rhynchosporium resistance. The frequencies and distributions of these novel and known QTL were superimposed on the regional origin of the landrace genotypes comprising the genome-wide association studies (GWAS) panel, highlighting the value of genetic resources as a source of diverse genetically controlled resistance to rhynchosporium. The detected QTL along with their linked genetic markers, could be exploited either directly for breeding purposes or for candidate gene identification in future studies.https://www.mdpi.com/2073-4395/12/4/782barleylandracesrhynchosporiumresistanceQTLGWAS |
| spellingShingle | Jean-Noël Thauvin Joanne Russell Dominique Vequaud Mark Looseley Micha Bayer Pierre-Marie Le Roux Pierre Pin Robbie Waugh Anna Avrova Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm Agronomy barley landraces rhynchosporium resistance QTL GWAS |
| title | Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm |
| title_full | Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm |
| title_fullStr | Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm |
| title_full_unstemmed | Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm |
| title_short | Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm |
| title_sort | genome wide association study for resistance to rhynchosporium in a diverse collection of spring barley germplasm |
| topic | barley landraces rhynchosporium resistance QTL GWAS |
| url | https://www.mdpi.com/2073-4395/12/4/782 |
| work_keys_str_mv | AT jeannoelthauvin genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm AT joannerussell genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm AT dominiquevequaud genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm AT marklooseley genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm AT michabayer genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm AT pierremarieleroux genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm AT pierrepin genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm AT robbiewaugh genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm AT annaavrova genomewideassociationstudyforresistancetorhynchosporiuminadiversecollectionofspringbarleygermplasm |