Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.)
Abstract Background Vitis vinifera L. is the most cultivated grapevine species worldwide. Erysiphe necator Sch., the causal agent of grape powdery mildew, is one of the main pathogens affecting viticulture. V. vinifera has little or no genetic resistances against E. necator and the grape industry is...
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03174-4 |
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| author | Tyrone Possamai Sabine Wiedemann-Merdinoglu Didier Merdinoglu Daniele Migliaro Gloria De Mori Guido Cipriani Riccardo Velasco Raffaele Testolin |
| author_facet | Tyrone Possamai Sabine Wiedemann-Merdinoglu Didier Merdinoglu Daniele Migliaro Gloria De Mori Guido Cipriani Riccardo Velasco Raffaele Testolin |
| author_sort | Tyrone Possamai |
| collection | DOAJ |
| description | Abstract Background Vitis vinifera L. is the most cultivated grapevine species worldwide. Erysiphe necator Sch., the causal agent of grape powdery mildew, is one of the main pathogens affecting viticulture. V. vinifera has little or no genetic resistances against E. necator and the grape industry is highly dependent on agrochemicals. Some Caucasian V. vinifera accessions have been reported to be resistant to E. necator and to have no genetic relationships to known sources of resistance to powdery mildew. The main purpose of this work was the study and mapping of the resistance to E. necator in the Caucasian grapes ‘Shavtsitska’ and ‘Tskhvedianis tetra’. Results The Caucasian varieties ‘Shavtsitska’ and ‘Tskhvedianis tetra’ showed a strong partial resistance to E. necator which segregated in two cross populations: the resistant genotypes delayed and limited the pathogen mycelium growth, sporulation intensity and number of conidia generated. A total of 184 seedlings of ‘Shavtsitska’ x ‘Glera’ population were genotyped through the Genotyping by Sequencing (GBS) technology and two high-density linkage maps were developed for the cross parents. The QTL analysis revealed a major resistance locus, explaining up to 80.15% of the phenotypic variance, on ‘Shavtsitska’ linkage group 13, which was associated with a reduced pathogen infection as well as an enhanced plant necrotic response. The genotyping of 105 Caucasian accessions with SSR markers flanking the QTL revealed that the resistant haplotype of ‘Shavtsitska’ was shared by ‘Tskhvedianis tetra’ and a total of 25 Caucasian grape varieties, suggesting a widespread presence of this resistance in the surveyed germplasm. The uncovered QTL was mapped in the region where the Ren1 locus of resistance to E. necator, identified in the V. vinifera ‘Kishmish vatkana’ and related grapes of Central Asia, is located. The genetic analysis conducted revealed that the Caucasian grapes in this study exhibit a resistant haplotype different from that of Central Asian grape accessions. Conclusions The QTL isolated in ‘Shavtsitska’ and present in the Caucasian V. vinifera varieties could be a new candidate gene of resistance to E. necator to use in breeding programmes. It co-localizes with the Ren1 locus but shows a different haplotype from that of grapevines of Central Asia. We therefore consider that the Caucasian resistance locus, named Ren1.2, contains a member of a cluster of R-genes, of which the region is rich, and to be linked with, or possibly allelic, to Ren1. |
| first_indexed | 2024-12-23T19:31:16Z |
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| id | doaj.art-39b17d3645424d8281aabf7210c9ea6b |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-23T19:31:16Z |
| publishDate | 2021-11-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-39b17d3645424d8281aabf7210c9ea6b2022-12-21T17:33:55ZengBMCBMC Plant Biology1471-22292021-11-0121112010.1186/s12870-021-03174-4Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.)Tyrone Possamai0Sabine Wiedemann-Merdinoglu1Didier Merdinoglu2Daniele Migliaro3Gloria De Mori4Guido Cipriani5Riccardo Velasco6Raffaele Testolin7Department of Agricultural, Food, Environmental and Animal Sciences, University of UdineINRAE, Université de Strasbourg, SVQV UMR-A 1131INRAE, Université de Strasbourg, SVQV UMR-A 1131CREA - Research Centre for Viticulture and EnologyDepartment of Agricultural, Food, Environmental and Animal Sciences, University of UdineDepartment of Agricultural, Food, Environmental and Animal Sciences, University of UdineCREA - Research Centre for Viticulture and EnologyDepartment of Agricultural, Food, Environmental and Animal Sciences, University of UdineAbstract Background Vitis vinifera L. is the most cultivated grapevine species worldwide. Erysiphe necator Sch., the causal agent of grape powdery mildew, is one of the main pathogens affecting viticulture. V. vinifera has little or no genetic resistances against E. necator and the grape industry is highly dependent on agrochemicals. Some Caucasian V. vinifera accessions have been reported to be resistant to E. necator and to have no genetic relationships to known sources of resistance to powdery mildew. The main purpose of this work was the study and mapping of the resistance to E. necator in the Caucasian grapes ‘Shavtsitska’ and ‘Tskhvedianis tetra’. Results The Caucasian varieties ‘Shavtsitska’ and ‘Tskhvedianis tetra’ showed a strong partial resistance to E. necator which segregated in two cross populations: the resistant genotypes delayed and limited the pathogen mycelium growth, sporulation intensity and number of conidia generated. A total of 184 seedlings of ‘Shavtsitska’ x ‘Glera’ population were genotyped through the Genotyping by Sequencing (GBS) technology and two high-density linkage maps were developed for the cross parents. The QTL analysis revealed a major resistance locus, explaining up to 80.15% of the phenotypic variance, on ‘Shavtsitska’ linkage group 13, which was associated with a reduced pathogen infection as well as an enhanced plant necrotic response. The genotyping of 105 Caucasian accessions with SSR markers flanking the QTL revealed that the resistant haplotype of ‘Shavtsitska’ was shared by ‘Tskhvedianis tetra’ and a total of 25 Caucasian grape varieties, suggesting a widespread presence of this resistance in the surveyed germplasm. The uncovered QTL was mapped in the region where the Ren1 locus of resistance to E. necator, identified in the V. vinifera ‘Kishmish vatkana’ and related grapes of Central Asia, is located. The genetic analysis conducted revealed that the Caucasian grapes in this study exhibit a resistant haplotype different from that of Central Asian grape accessions. Conclusions The QTL isolated in ‘Shavtsitska’ and present in the Caucasian V. vinifera varieties could be a new candidate gene of resistance to E. necator to use in breeding programmes. It co-localizes with the Ren1 locus but shows a different haplotype from that of grapevines of Central Asia. We therefore consider that the Caucasian resistance locus, named Ren1.2, contains a member of a cluster of R-genes, of which the region is rich, and to be linked with, or possibly allelic, to Ren1.https://doi.org/10.1186/s12870-021-03174-4Resistance genesRen lociGrape breedingPowdery mildew phenotyping |
| spellingShingle | Tyrone Possamai Sabine Wiedemann-Merdinoglu Didier Merdinoglu Daniele Migliaro Gloria De Mori Guido Cipriani Riccardo Velasco Raffaele Testolin Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.) BMC Plant Biology Resistance genes Ren loci Grape breeding Powdery mildew phenotyping |
| title | Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.) |
| title_full | Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.) |
| title_fullStr | Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.) |
| title_full_unstemmed | Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.) |
| title_short | Construction of a high-density genetic map and detection of a major QTL of resistance to powdery mildew (Erysiphe necator Sch.) in Caucasian grapes (Vitis vinifera L.) |
| title_sort | construction of a high density genetic map and detection of a major qtl of resistance to powdery mildew erysiphe necator sch in caucasian grapes vitis vinifera l |
| topic | Resistance genes Ren loci Grape breeding Powdery mildew phenotyping |
| url | https://doi.org/10.1186/s12870-021-03174-4 |
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