Baculovirus Expression and Functional Analysis of Vpa2 Proteins from <em>Bacillus thuringiensis</em>

Baculovirus Expression and Functional Analysis of Vpa2 Proteins from <em>Bacillus thuringiensis</em>

The mode of action underlying the insecticidal activity of the <i>Bacillus thuringiensis</i> (Bt) binary pesticidal protein Vpb/Vpa (formerly Vip1/Vip2) is uncertain. In this study, three recombinant baculoviruses were constructed using Bac-to-Bac technology to express Vpa2Ac1 and two no...

Full description

Bibliographic Details
Main Authors:	Oihane Simón, Leopoldo Palma, Ana Beatriz Fernández, Trevor Williams, Primitivo Caballero
Format:	Article
Language:	English
Published:	MDPI AG 2020-08-01
Series:	Toxins
Subjects:	vegetative insecticidal proteins Vpb/Vpa entomopathogen ADP-ribosyltransferase recombinant baculovirus lepidopteran cells
Online Access:	https://www.mdpi.com/2072-6651/12/9/543

Similar Items

Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)
by: Fernando Barnabe Cerqueira, et al.
Published: (2016-12-01)

Baculovirus Induced Transcripts in Hemocytes from the Larvae of Heliothis virescens
by: Holly J.R. Popham, et al.
Published: (2011-10-01)

<i>Bacillus thuringiensis</i> Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against <i>Anthonomus grandis</i> Boheman (Coleoptera: Curculionidae)
by: Diego Herman Sauka, et al.
Published: (2023-12-01)

Analysis of variability of concentrations of valproic acid (VPA) and its selected metabolites in the blood serum of patients treated with VPA and patients hospitalized because of VPA poisoning
by: Jolanta Wilimowska, et al.
Published: (2015-03-01)

Research Progress on Mono-ADP-Ribosyltransferases in Human Cell Biology
by: Yujie Gan, et al.
Published: (2022-05-01)

Baculovirus expression vector system for production of viral vaccines /
by: 300419 Vlak, J. M., et al.

The role of baculoviruses in controlling insect pests: A review
by: Yohannes Gelaye, et al.
Published: (2023-12-01)

Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody
by: Astrid Rohrbeck, et al.
Published: (2016-04-01)

Preliminary study on the optimization of recombinant human transferrin expression in insect cells baculovirus system /
by: 257680 Ongkudon, Clarence M., et al.
Published: (2004)

Cell Death Signaling Pathway Induced by Cholix Toxin, a Cytotoxin and eEF2 ADP-Ribosyltransferase Produced by <i>Vibrio cholerae</i>
by: Kohei Ogura, et al.
Published: (2020-12-01)

High Norepinephrine State Induces Growth of Colorectal Cancer Cells via ADP-Ribosyltransferase 1 in Type 2 Diabetes Mellitus
by: Wenwen Chen, et al.
Published: (2023-11-01)

Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ
by: Dominic J. Pollard, et al.
Published: (2018-05-01)

A manual of methods for baculovirus vectors and insect cell culture procedures /
by: Summers, Max D, et al.
Published: (1987)

The ADP-Ribosyl-Transferases Diphtheria Toxin-Like (ARTDs) Family: An Overview
by: Maria Di Girolamo, et al.
Published: (2018-05-01)

Post-Transcriptional Regulation of PARP7 Protein Stability Is Controlled by Androgen Signaling
by: Teddy Kamata, et al.
Published: (2021-02-01)

The Binary Toxin CDT of Clostridium difficile as a Tool for Intracellular Delivery of Bacterial Glucosyltransferase Domains
by: Lara-Antonia Beer, et al.
Published: (2018-06-01)

PARPs and ADP-Ribosylation in Chronic Inflammation: A Focus on Macrophages
by: Diego V. Santinelli-Pestana, et al.
Published: (2023-07-01)

Baculoviruses as gene expression vectors
by: 273936 Miller, Lois K.

Baculovirus Surface Display of Immunogenic Proteins for Vaccine Development
by: Balraj Premanand, et al.
Published: (2018-05-01)

General developmental health in the VPA-rat model of autism
by: Monica Regina Favre, et al.
Published: (2013-07-01)

VPA/PLGA microfibers produced by coaxial electrospinning for the treatment of central nervous system injury
by: K.P. Reis, et al.
Published: (2020-04-01)

Vegetable waste extracts as enhancers of baculovirus infections
by: Blanca Martínez-Inda, et al.
Published: (2023-12-01)

A novel predicted ADP-ribosyltransferase-like family conserved in eukaryotic evolution
by: Zbigniew Wyżewski, et al.
Published: (2021-03-01)

The zinc-binding motif in tankyrases is required for the structural integrity of the catalytic ADP-ribosyltransferase domain
by: Sven T. Sowa, et al.
Published: (2022-03-01)

Homologous recombination risk in baculovirus expression vector system
by: Jianan Huang, et al.
Published: (2022-11-01)

The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1.1 through protein kinase C delta
by: Yuemin Tian, et al.
Published: (2020-10-01)

Draft Genome Sequences of Two Bacillus thuringiensis Strains and Characterization of a Putative 41.9-kDa Insecticidal Toxin
by: Leopoldo Palma, et al.
Published: (2014-04-01)

Molecular characterization and toxicity evaluation of indigenous Bacillus thuringiensis isolates against key lepidopteran insect pests
by: Rajadurai Gothandaraman, et al.
Published: (2022-12-01)

Effecient transfection of insect cell with Baculovirus DNA using electroporation /
by: 301771 Mann, Susan G., et al.

Establishment of a Novel Baculovirus–Silkworm Expression System
by: Junhong Wei, et al.
Published: (2022-05-01)

Linearzation of baculovirus DNA enhances the recovery of recombinant virus expression vectors /
by: 295772 Kitts, Paul A., et al.

Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors/
by: 295772 Kitts, Paul A., et al.

Acupuncture alters the intestinal microbiota in mice with valproic acid (VPA)-induced autism-like behavior
by: Zirong Bai, et al.
Published: (2022-12-01)

Common Mechanism for Target Specificity of Protein- and DNA-Targeting ADP-Ribosyltransferases
by: Toru Yoshida, et al.
Published: (2021-01-01)

SpyB, a small heme-binding protein, affects the composition of the cell wall in Streptococcus pyogenes
by: Rebecca J Edgar, et al.
Published: (2016-10-01)

Several New Putative Bacterial ADP-Ribosyltransferase Toxins Are Revealed from In Silico Data Mining, Including the Novel Toxin Vorin, Encoded by the Fire Blight Pathogen <em>Erwinia amylovora</em>
by: Olivier Tremblay, et al.
Published: (2020-12-01)

Mortality of Oryzophagus oryzae (Costa Lima, 1936) (Coleoptera: Curculionidae) and Spodoptera frugiperda (J E Smith, 1797) (Lepidoptera: Noctuidae) Larvae Exposed to Bacillus thuringiensis and Extracts of Melia azedarach
by: Diouneia Lisiane Berlitz, et al.
Published: (2012-10-01)

The History of Baculovirology in Africa
by: Sean Moore, et al.
Published: (2023-07-01)

Study on the Protective Immunity Induced by Pseudotyped Baculovirus Expressing the E Protein of Tembusu Virus in Ducklings
by: Zheng Ni, et al.
Published: (2023-06-01)

Recombinant baculovirus expressing the FrC-OVA protein induces protective antitumor immunity in an EG7-OVA mouse model
by: Keigo Kondou, et al.
Published: (2019-10-01)