Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts
Antibacterial, antibiofilm and antioxidant activity of acetone, ethanol, ethyl acetate and diethyl ether extracts of P. nodosus Poir. were tested. In vitro antibacterial activity was investigated by microdilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (...
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University of Kragujevac, Faculty of Science
2014-01-01
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| Series: | Kragujevac Journal of Science |
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| Online Access: | http://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2014/1450-96361436137D.pdf |
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| author | Đurđević Jelena Vasić Sava Radojević Ivana Đelić Gorica Čomić Ljiljana |
| author_facet | Đurđević Jelena Vasić Sava Radojević Ivana Đelić Gorica Čomić Ljiljana |
| author_sort | Đurđević Jelena |
| collection | DOAJ |
| description | Antibacterial, antibiofilm and antioxidant activity of acetone, ethanol, ethyl acetate and diethyl ether extracts of P. nodosus Poir. were tested. In vitro antibacterial activity was investigated by microdilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. The values were in the range from 5 to > 5 mg/ml. Antibiofilm activity was tested by crystal violet assay. The biofilm inhibitory concentration (BIC) values were from 2.03 to > 10 mg/ml. The best results against biofilm formation had ethanol and ethyl acetate extracts. The total phenolic content was established by using Folin-Ciocalteu method. The highest total phenol content was found in the ethyl acetate extract (28.45 mg GA/g) while the lowest content in the ethanol extract (22.74 mg GA/g). The concentrations of flavonoids were determined using aluminium chloride method. The concentration of total flavonoid content was 102.09 mg RU/g for the ethyl acetate extract, while the lowest content was noticed in diethyl ether extract (32.93 mg RU/g). The antioxidant activity was determined by the DPPH free radicals assay. Antioxidative efficiency was in the range from 641.77 to 8811.63 μg/ml. The tested extracts showed weak antioxidant activity (AAI < 0.5). |
| first_indexed | 2024-04-14T00:23:35Z |
| format | Article |
| id | doaj.art-39b70734738642feac3ad5ec538ad7a0 |
| institution | Directory Open Access Journal |
| issn | 1450-9636 2466-5509 |
| language | English |
| last_indexed | 2024-04-14T00:23:35Z |
| publishDate | 2014-01-01 |
| publisher | University of Kragujevac, Faculty of Science |
| record_format | Article |
| series | Kragujevac Journal of Science |
| spelling | doaj.art-39b70734738642feac3ad5ec538ad7a02022-12-22T02:22:52ZengUniversity of Kragujevac, Faculty of ScienceKragujevac Journal of Science1450-96362466-55092014-01-0120143613714410.5937/KgJSci1436137D1450-96361436137DAnibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extractsĐurđević Jelena0Vasić Sava1Radojević Ivana2Đelić Gorica3Čomić Ljiljana4University of Kragujevac, Faculty of Science - Institute of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science - Institute of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science - Institute of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science - Institute of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science - Institute of Biology and Ecology, Kragujevac, SerbiaAntibacterial, antibiofilm and antioxidant activity of acetone, ethanol, ethyl acetate and diethyl ether extracts of P. nodosus Poir. were tested. In vitro antibacterial activity was investigated by microdilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. The values were in the range from 5 to > 5 mg/ml. Antibiofilm activity was tested by crystal violet assay. The biofilm inhibitory concentration (BIC) values were from 2.03 to > 10 mg/ml. The best results against biofilm formation had ethanol and ethyl acetate extracts. The total phenolic content was established by using Folin-Ciocalteu method. The highest total phenol content was found in the ethyl acetate extract (28.45 mg GA/g) while the lowest content in the ethanol extract (22.74 mg GA/g). The concentrations of flavonoids were determined using aluminium chloride method. The concentration of total flavonoid content was 102.09 mg RU/g for the ethyl acetate extract, while the lowest content was noticed in diethyl ether extract (32.93 mg RU/g). The antioxidant activity was determined by the DPPH free radicals assay. Antioxidative efficiency was in the range from 641.77 to 8811.63 μg/ml. The tested extracts showed weak antioxidant activity (AAI < 0.5).http://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2014/1450-96361436137D.pdfPotamogeton nodosusphenolicsflavonoidsDPPHantibacterial activitybiofilm |
| spellingShingle | Đurđević Jelena Vasić Sava Radojević Ivana Đelić Gorica Čomić Ljiljana Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts Kragujevac Journal of Science Potamogeton nodosus phenolics flavonoids DPPH antibacterial activity biofilm |
| title | Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts |
| title_full | Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts |
| title_fullStr | Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts |
| title_full_unstemmed | Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts |
| title_short | Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts |
| title_sort | anibacterial antibiofilm and antioxidant activity of potamogeton nodosus poir extracts |
| topic | Potamogeton nodosus phenolics flavonoids DPPH antibacterial activity biofilm |
| url | http://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2014/1450-96361436137D.pdf |
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