<em>In vitro</em> multiplication of clone 'Viequera' (<em>Xanthosoma</em> spp.) in semi-automated culture systems

In Cuba, theXanthosoma genus is highly demanded in the preference of the population in relation to other edible aroids. The temporary immersion system (TIS) may constitute a micropropagation alternative to obtain high quality seed in this crop. The effect of two Semi-automated Culture Systems (SIT)...

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Main Authors: Arletys Santos Pino, Manuel Cabrera Jova, Rafael Gómez-Kosky, Jorge López Torres, Diosdada Galvez Guerra, Damicela Reinaldo, Aymé Rayas Cabrera, Milagros Basail Pérez, Víctor Medero Vega, Yoel Beovidez García
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2012-04-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/162
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author Arletys Santos Pino
Manuel Cabrera Jova
Rafael Gómez-Kosky
Jorge López Torres
Diosdada Galvez Guerra
Damicela Reinaldo
Aymé Rayas Cabrera
Milagros Basail Pérez
Víctor Medero Vega
Yoel Beovidez García
author_facet Arletys Santos Pino
Manuel Cabrera Jova
Rafael Gómez-Kosky
Jorge López Torres
Diosdada Galvez Guerra
Damicela Reinaldo
Aymé Rayas Cabrera
Milagros Basail Pérez
Víctor Medero Vega
Yoel Beovidez García
author_sort Arletys Santos Pino
collection DOAJ
description In Cuba, theXanthosoma genus is highly demanded in the preference of the population in relation to other edible aroids. The temporary immersion system (TIS) may constitute a micropropagation alternative to obtain high quality seed in this crop. The effect of two Semi-automated Culture Systems (SIT) and Constant Immersion Systems (CIS) with aeration through continuous bubbling in culture medium during the multiplication stage of axillaries shoot buds was evaluated. Results allowed demonstrating superiority in the efficiency of SIT regarding CIS in the sprout multiplication of clone ‘Viequera’. Axillaries sprout buds cultivated in TIS showed the best results in the multiplication compared with CIS and the static culture system with passive renewal of the internal atmosphere was used as control. The culture conditions created in the temporary immersion system forin vitro multiplication of axillaries sprout buds achievedthe highest multiplication coefficient(9.56). Thisin vitro culture system will allow increasing the multiplication coefficients in the micropropagation for seed productionin this genus. Keyword: multiplication coefficient, micropropagation, temporary immersion system
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spelling doaj.art-39bcc8cfe5f14dd9a45a4da9e05f16ba2022-12-22T00:48:13ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472012-04-01122139<em>In vitro</em> multiplication of clone 'Viequera' (<em>Xanthosoma</em> spp.) in semi-automated culture systemsArletys Santos PinoManuel Cabrera JovaRafael Gómez-KoskyJorge López TorresDiosdada Galvez GuerraDamicela ReinaldoAymé Rayas CabreraMilagros Basail PérezVíctor Medero VegaYoel Beovidez GarcíaIn Cuba, theXanthosoma genus is highly demanded in the preference of the population in relation to other edible aroids. The temporary immersion system (TIS) may constitute a micropropagation alternative to obtain high quality seed in this crop. The effect of two Semi-automated Culture Systems (SIT) and Constant Immersion Systems (CIS) with aeration through continuous bubbling in culture medium during the multiplication stage of axillaries shoot buds was evaluated. Results allowed demonstrating superiority in the efficiency of SIT regarding CIS in the sprout multiplication of clone ‘Viequera’. Axillaries sprout buds cultivated in TIS showed the best results in the multiplication compared with CIS and the static culture system with passive renewal of the internal atmosphere was used as control. The culture conditions created in the temporary immersion system forin vitro multiplication of axillaries sprout buds achievedthe highest multiplication coefficient(9.56). Thisin vitro culture system will allow increasing the multiplication coefficients in the micropropagation for seed productionin this genus. Keyword: multiplication coefficient, micropropagation, temporary immersion systemhttps://revista.ibp.co.cu/index.php/BV/article/view/162
spellingShingle Arletys Santos Pino
Manuel Cabrera Jova
Rafael Gómez-Kosky
Jorge López Torres
Diosdada Galvez Guerra
Damicela Reinaldo
Aymé Rayas Cabrera
Milagros Basail Pérez
Víctor Medero Vega
Yoel Beovidez García
<em>In vitro</em> multiplication of clone 'Viequera' (<em>Xanthosoma</em> spp.) in semi-automated culture systems
Biotecnología Vegetal
title <em>In vitro</em> multiplication of clone 'Viequera' (<em>Xanthosoma</em> spp.) in semi-automated culture systems
title_full <em>In vitro</em> multiplication of clone 'Viequera' (<em>Xanthosoma</em> spp.) in semi-automated culture systems
title_fullStr <em>In vitro</em> multiplication of clone 'Viequera' (<em>Xanthosoma</em> spp.) in semi-automated culture systems
title_full_unstemmed <em>In vitro</em> multiplication of clone 'Viequera' (<em>Xanthosoma</em> spp.) in semi-automated culture systems
title_short <em>In vitro</em> multiplication of clone 'Viequera' (<em>Xanthosoma</em> spp.) in semi-automated culture systems
title_sort em in vitro em multiplication of clone viequera em xanthosoma em spp in semi automated culture systems
url https://revista.ibp.co.cu/index.php/BV/article/view/162
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