Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C
Total number of forty testes from twenty Sudani camels (Camelus dromedarius) were used in the present study (>5-10 years old and 500-600 kg body weight). The experimental work was executed to define the effect of green laser irradiation with short-wavelength 532 nm and continuous wave from a diod...
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2021-04-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_134861_e466f866c1cff3ef6357eb4691dc8cc0.pdf |
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| author | Ahmed Seioudy Esmat Abdalla Alaa Zeidan Farouk Khalil Zienab Abdel-Salam Magdy Badr Maiada Allam |
| author_facet | Ahmed Seioudy Esmat Abdalla Alaa Zeidan Farouk Khalil Zienab Abdel-Salam Magdy Badr Maiada Allam |
| author_sort | Ahmed Seioudy |
| collection | DOAJ |
| description | Total number of forty testes from twenty Sudani camels (Camelus dromedarius) were used in the present study (>5-10 years old and 500-600 kg body weight). The experimental work was executed to define the effect of green laser irradiation with short-wavelength 532 nm and continuous wave from a diode laser light with a total output power of 3 mW on epididymal camel spermatozoa quality at different exposure times of 0 (control, non-irradiated), 2, 4, 6, 8, and 10 min. Following irradiation, the percentages of motile spermatozoa, storagability, viability, and acrosomal damage were assessed of the epididymal camel spermatozoa stored at 5°C for 4 days. Epididymal spermatozoa was diluted with lactose-yolk-citrate (LYC) extender. The obtained results showed that the highest (P<0.05) value of the percentages of motile and storagability of spermatozoa was recorded with spermatozoa exposed to 6 min of laser irradiation and the lowest (P<0.05) value was recorded with the control group. Otherwise, the highest (P<0.05) value of the percentages of dead and acrosomal damage of spermatozoa was recorded with spermatozoa exposed to 10 min and the lowest (P<0.05) value was recorded with 2 min. The advancement of storage time at 5°C decreased (P<0.05) the percentages of motile and storagability of spermatozoa, while increased (P<0.05) the percentages of dead and acrosomal damage of spermatozoa during storage at 5°C for 4 days. Consequently, enhancing the artificial insemination program can be achieved using the laser irradiation which is considered a cost-effective technique for improving semen quality. The profitable effects of laser irradiation on epididymal camel spermatozoa quality raised the motile spermatozoa, storagability, livability, acrosomal integrity which consider an indicator to improve mitochondrial function which extends the survival of spermatozoa |
| first_indexed | 2024-04-25T01:55:05Z |
| format | Article |
| id | doaj.art-39bda7ddad2c4df1bfa6e6869536c931 |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:55:05Z |
| publishDate | 2021-04-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-39bda7ddad2c4df1bfa6e6869536c9312024-03-07T17:34:16ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852021-04-0129130731410.21608/ajs.2021.48967.1290134861Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°CAhmed Seioudy0Esmat Abdalla1Alaa Zeidan2Farouk Khalil3Zienab Abdel-Salam4Magdy Badr5Maiada Allam6Camel Research Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.Animal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.Camel Research Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.Animal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.Animal Reproduction Research Institute, Agricultural Research Center, Giza, Egypt.Automated El-Bassatein Abattoir, Directorate of Veterinary Medicine, Cairo, Egypt.Total number of forty testes from twenty Sudani camels (Camelus dromedarius) were used in the present study (>5-10 years old and 500-600 kg body weight). The experimental work was executed to define the effect of green laser irradiation with short-wavelength 532 nm and continuous wave from a diode laser light with a total output power of 3 mW on epididymal camel spermatozoa quality at different exposure times of 0 (control, non-irradiated), 2, 4, 6, 8, and 10 min. Following irradiation, the percentages of motile spermatozoa, storagability, viability, and acrosomal damage were assessed of the epididymal camel spermatozoa stored at 5°C for 4 days. Epididymal spermatozoa was diluted with lactose-yolk-citrate (LYC) extender. The obtained results showed that the highest (P<0.05) value of the percentages of motile and storagability of spermatozoa was recorded with spermatozoa exposed to 6 min of laser irradiation and the lowest (P<0.05) value was recorded with the control group. Otherwise, the highest (P<0.05) value of the percentages of dead and acrosomal damage of spermatozoa was recorded with spermatozoa exposed to 10 min and the lowest (P<0.05) value was recorded with 2 min. The advancement of storage time at 5°C decreased (P<0.05) the percentages of motile and storagability of spermatozoa, while increased (P<0.05) the percentages of dead and acrosomal damage of spermatozoa during storage at 5°C for 4 days. Consequently, enhancing the artificial insemination program can be achieved using the laser irradiation which is considered a cost-effective technique for improving semen quality. The profitable effects of laser irradiation on epididymal camel spermatozoa quality raised the motile spermatozoa, storagability, livability, acrosomal integrity which consider an indicator to improve mitochondrial function which extends the survival of spermatozoahttps://ajs.journals.ekb.eg/article_134861_e466f866c1cff3ef6357eb4691dc8cc0.pdfcamelsepididymal spermatozoagreen laser irradiationviabilitystoragability |
| spellingShingle | Ahmed Seioudy Esmat Abdalla Alaa Zeidan Farouk Khalil Zienab Abdel-Salam Magdy Badr Maiada Allam Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C Arab Universities Journal of Agricultural Sciences camels epididymal spermatozoa green laser irradiation viability storagability |
| title | Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C |
| title_full | Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C |
| title_fullStr | Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C |
| title_full_unstemmed | Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C |
| title_short | Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C |
| title_sort | effect of green laser irradiation on epididymal camel spermatozoa quality stored at 5°c |
| topic | camels epididymal spermatozoa green laser irradiation viability storagability |
| url | https://ajs.journals.ekb.eg/article_134861_e466f866c1cff3ef6357eb4691dc8cc0.pdf |
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