Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing
Abstract Background Diabetic wounds are one of the most important issues in diabetic patients. It seems that Juglans regia L. leaf with antioxidant and anti-inflammatory potentials can be profitable for healing of diabetic wounds. The aim of present study was to investigate the topical administratio...
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-022-03735-6 |
| _version_ | 1797996133959598080 |
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| author | Davood Nasiry Ali Reza Khalatbary Alireza Ghaemi Mohammad Ali Ebrahimzadeh Mohammad Hossein Hosseinzadeh |
| author_facet | Davood Nasiry Ali Reza Khalatbary Alireza Ghaemi Mohammad Ali Ebrahimzadeh Mohammad Hossein Hosseinzadeh |
| author_sort | Davood Nasiry |
| collection | DOAJ |
| description | Abstract Background Diabetic wounds are one of the most important issues in diabetic patients. It seems that Juglans regia L. leaf with antioxidant and anti-inflammatory potentials can be profitable for healing of diabetic wounds. The aim of present study was to investigate the topical administration of Juglans regia L. leaf extract in diabetic wound healing. Methods Seventy-five diabetic male rats were randomly divided into 5 groups (n = 15), including: untreated (Control) group, Eucerin group, 2% Juglans regia L. ointment (JRL 2%) group, 5% Juglans regia L. ointment (JRL 5%) group, and Phenytoin group as a reference drug. Sampling was performed at days 7, 14, and 21 after surgery. Evaluation tests included stereology, immunohistochemistry, molecular, and biomechanical. Results Our results showed that the wound closure rate, volumes of newly formed of epidermis and dermis, density of fibroblasts and blood vessels, collagen deposition, density of proliferation cells, expression levels of TGF-β and VEGF genes, and biomechanical characteristics were significantly higher in extract groups compared to control and eucerin groups, however, these changes were considerable in the JRL 5% group (P < 0.05). This is while that the density of neutrophils and expression levels of TNF-α and IL-1β genes in the extract groups, especially in the JRL 5% group, were significantly reduced compared to control and eucerin groups (P < 0.05). Conclusion Topical administration of Juglans regia L. leaf extract, especially in 5% concentration, considerably accelerates diabetic wound healing. |
| first_indexed | 2024-04-11T10:11:36Z |
| format | Article |
| id | doaj.art-39bf6f98884544209fe315ea38f52a86 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-04-11T10:11:36Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-39bf6f98884544209fe315ea38f52a862022-12-22T04:30:04ZengBMCBMC Complementary Medicine and Therapies2662-76712022-10-0122111210.1186/s12906-022-03735-6Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healingDavood Nasiry0Ali Reza Khalatbary1Alireza Ghaemi2Mohammad Ali Ebrahimzadeh3Mohammad Hossein Hosseinzadeh4Amol Faculty of Paramedicine, Mazandaran University of Medical SciencesCellular and Molecular Research Center, Mazandaran University of Medical SciencesDepartment of Nutrition, Health Sciences Research Center, Faculty of Public Health, Addiction Institute, Mazandaran University of Medical SciencesPharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical SciencesFaculty of Pharmacy, Mazandaran University of Medical SciencesAbstract Background Diabetic wounds are one of the most important issues in diabetic patients. It seems that Juglans regia L. leaf with antioxidant and anti-inflammatory potentials can be profitable for healing of diabetic wounds. The aim of present study was to investigate the topical administration of Juglans regia L. leaf extract in diabetic wound healing. Methods Seventy-five diabetic male rats were randomly divided into 5 groups (n = 15), including: untreated (Control) group, Eucerin group, 2% Juglans regia L. ointment (JRL 2%) group, 5% Juglans regia L. ointment (JRL 5%) group, and Phenytoin group as a reference drug. Sampling was performed at days 7, 14, and 21 after surgery. Evaluation tests included stereology, immunohistochemistry, molecular, and biomechanical. Results Our results showed that the wound closure rate, volumes of newly formed of epidermis and dermis, density of fibroblasts and blood vessels, collagen deposition, density of proliferation cells, expression levels of TGF-β and VEGF genes, and biomechanical characteristics were significantly higher in extract groups compared to control and eucerin groups, however, these changes were considerable in the JRL 5% group (P < 0.05). This is while that the density of neutrophils and expression levels of TNF-α and IL-1β genes in the extract groups, especially in the JRL 5% group, were significantly reduced compared to control and eucerin groups (P < 0.05). Conclusion Topical administration of Juglans regia L. leaf extract, especially in 5% concentration, considerably accelerates diabetic wound healing.https://doi.org/10.1186/s12906-022-03735-6Diabetic woundWound healingJuglans regia leaf extractCollagen biosynthesis |
| spellingShingle | Davood Nasiry Ali Reza Khalatbary Alireza Ghaemi Mohammad Ali Ebrahimzadeh Mohammad Hossein Hosseinzadeh Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing BMC Complementary Medicine and Therapies Diabetic wound Wound healing Juglans regia leaf extract Collagen biosynthesis |
| title | Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing |
| title_full | Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing |
| title_fullStr | Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing |
| title_full_unstemmed | Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing |
| title_short | Topical administration of Juglans regia L. leaf extract accelerates diabetic wound healing |
| title_sort | topical administration of juglans regia l leaf extract accelerates diabetic wound healing |
| topic | Diabetic wound Wound healing Juglans regia leaf extract Collagen biosynthesis |
| url | https://doi.org/10.1186/s12906-022-03735-6 |
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