High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.)
Biofortification is a sustainable strategy to alleviate micronutrient deficiency in humans. It is necessary to improve grain zinc (GZnC) and iron concentrations (GFeC) in wheat based on genetic knowledge. However, the precise dissection of the genetic architecture underlying GZnC and GFeC remains ch...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.840614/full |
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| author | Jingyang Tong Cong Zhao Mengjing Sun Luping Fu Jie Song Dan Liu Yelun Zhang Jianmin Zheng Zongjun Pu Lianzheng Liu Awais Rasheed Awais Rasheed Ming Li Xianchun Xia Zhonghu He Zhonghu He Yuanfeng Hao |
| author_facet | Jingyang Tong Cong Zhao Mengjing Sun Luping Fu Jie Song Dan Liu Yelun Zhang Jianmin Zheng Zongjun Pu Lianzheng Liu Awais Rasheed Awais Rasheed Ming Li Xianchun Xia Zhonghu He Zhonghu He Yuanfeng Hao |
| author_sort | Jingyang Tong |
| collection | DOAJ |
| description | Biofortification is a sustainable strategy to alleviate micronutrient deficiency in humans. It is necessary to improve grain zinc (GZnC) and iron concentrations (GFeC) in wheat based on genetic knowledge. However, the precise dissection of the genetic architecture underlying GZnC and GFeC remains challenging. In this study, high-resolution genome-wide association studies were conducted for GZnC and GFeC by three different models using 166 wheat cultivars and 373,106 polymorphic markers from the wheat 660K and 90K single nucleotide polymorphism (SNP) arrays. Totally, 25 and 16 stable loci were detected for GZnC and GFeC, respectively. Among them, 17 loci for GZnC and 8 for GFeC are likely to be new quantitative trait locus/loci (QTL). Based on gene annotations and expression profiles, 28 promising candidate genes were identified for Zn/Fe uptake (8), transport (11), storage (3), and regulations (6). Of them, 11 genes were putative wheat orthologs of known Arabidopsis and rice genes related to Zn/Fe homeostasis. A brief model, such as genes related to Zn/Fe homeostasis from root uptake, xylem transport to the final seed storage was proposed in wheat. Kompetitive allele-specific PCR (KASP) markers were successfully developed for two major QTL of GZnC on chromosome arms 3AL and 7AL, respectively, which were independent of thousand kernel weight and plant height. The 3AL QTL was further validated in a bi-parental population under multi-environments. A wheat multidrug and toxic compound extrusion (MATE) transporter TraesCS3A01G499300, the ortholog of rice gene OsPEZ2, was identified as a potential candidate gene. This study has advanced our knowledge of the genetic basis underlying GZnC and GFeC in wheat and provides valuable markers and candidate genes for wheat biofortification. |
| first_indexed | 2024-12-11T15:19:52Z |
| format | Article |
| id | doaj.art-39bfa5f89c6848629766d2e2a269ca02 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T15:19:52Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-39bfa5f89c6848629766d2e2a269ca022022-12-22T01:00:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.840614840614High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.)Jingyang Tong0Cong Zhao1Mengjing Sun2Luping Fu3Jie Song4Dan Liu5Yelun Zhang6Jianmin Zheng7Zongjun Pu8Lianzheng Liu9Awais Rasheed10Awais Rasheed11Ming Li12Xianchun Xia13Zhonghu He14Zhonghu He15Yuanfeng Hao16Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaThe Key Laboratory of Crop Genetics and Breeding of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, ChinaResearch Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, ChinaInternational Maize and Wheat Improvement Center (CIMMYT) China Office, Beijing, ChinaDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad, PakistanInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInternational Maize and Wheat Improvement Center (CIMMYT) China Office, Beijing, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaBiofortification is a sustainable strategy to alleviate micronutrient deficiency in humans. It is necessary to improve grain zinc (GZnC) and iron concentrations (GFeC) in wheat based on genetic knowledge. However, the precise dissection of the genetic architecture underlying GZnC and GFeC remains challenging. In this study, high-resolution genome-wide association studies were conducted for GZnC and GFeC by three different models using 166 wheat cultivars and 373,106 polymorphic markers from the wheat 660K and 90K single nucleotide polymorphism (SNP) arrays. Totally, 25 and 16 stable loci were detected for GZnC and GFeC, respectively. Among them, 17 loci for GZnC and 8 for GFeC are likely to be new quantitative trait locus/loci (QTL). Based on gene annotations and expression profiles, 28 promising candidate genes were identified for Zn/Fe uptake (8), transport (11), storage (3), and regulations (6). Of them, 11 genes were putative wheat orthologs of known Arabidopsis and rice genes related to Zn/Fe homeostasis. A brief model, such as genes related to Zn/Fe homeostasis from root uptake, xylem transport to the final seed storage was proposed in wheat. Kompetitive allele-specific PCR (KASP) markers were successfully developed for two major QTL of GZnC on chromosome arms 3AL and 7AL, respectively, which were independent of thousand kernel weight and plant height. The 3AL QTL was further validated in a bi-parental population under multi-environments. A wheat multidrug and toxic compound extrusion (MATE) transporter TraesCS3A01G499300, the ortholog of rice gene OsPEZ2, was identified as a potential candidate gene. This study has advanced our knowledge of the genetic basis underlying GZnC and GFeC in wheat and provides valuable markers and candidate genes for wheat biofortification.https://www.frontiersin.org/articles/10.3389/fpls.2022.840614/fullbiofortificationcandidate genesGWASironzinc |
| spellingShingle | Jingyang Tong Cong Zhao Mengjing Sun Luping Fu Jie Song Dan Liu Yelun Zhang Jianmin Zheng Zongjun Pu Lianzheng Liu Awais Rasheed Awais Rasheed Ming Li Xianchun Xia Zhonghu He Zhonghu He Yuanfeng Hao High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.) Frontiers in Plant Science biofortification candidate genes GWAS iron zinc |
| title | High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.) |
| title_full | High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.) |
| title_fullStr | High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.) |
| title_full_unstemmed | High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.) |
| title_short | High Resolution Genome Wide Association Studies Reveal Rich Genetic Architectures of Grain Zinc and Iron in Common Wheat (Triticum aestivum L.) |
| title_sort | high resolution genome wide association studies reveal rich genetic architectures of grain zinc and iron in common wheat triticum aestivum l |
| topic | biofortification candidate genes GWAS iron zinc |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.840614/full |
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