Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer
Abstract Background In colorectal cancer (CRC), the normal tissue adjacent to tumor (NAT) communicates actively with the tumor. Adult stem cells from the colon play a crucial role in the development of the colonic epithelium. In the tumor microenvironment, however, it is unclear what changes have oc...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-08-01
|
| Series: | Cell Communication and Signaling |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12964-023-01140-1 |
| _version_ | 1827875444888698880 |
|---|---|
| author | Yuanyuan Zhao Mengmeng Guo Fuqiang Zhao Qian Liu Xia Wang |
| author_facet | Yuanyuan Zhao Mengmeng Guo Fuqiang Zhao Qian Liu Xia Wang |
| author_sort | Yuanyuan Zhao |
| collection | DOAJ |
| description | Abstract Background In colorectal cancer (CRC), the normal tissue adjacent to tumor (NAT) communicates actively with the tumor. Adult stem cells from the colon play a crucial role in the development of the colonic epithelium. In the tumor microenvironment, however, it is unclear what changes have occurred in colonic stem cells derived from NAT. Methods Using an intestinal stem cell culture system, we cultured colonic cells from NAT and paired CRC tissue, as well as cells from healthy tissue (HLT). Clonogenicity and differentiation ability were used to compare the function of clones from NAT, HLT and CRC tissues. RNA high-throughput sequencing of these clones was used to identify the molecular characteristics of NAT-derived clones. Coculture of clones from HLT and CRC was used to assess molecular changes. Results We found that the morphological characteristics, clonogenic ability, and differentiation ability of NAT-derived clones were consistent with those of HLT-derived clones. However, NAT-derived clones changed at the molecular level. A number of genes were specifically activated in NAT. NAT-derived clones enriched pathways related to inflammation and fibrosis, including epithelial mesenchymal transition (EMT) pathway and TGF-beta signaling pathway. Our results also confirmed that NAT-derived clones could recruit fibroblasts in mice. In addition, HLT-derived clones showed high expression of FOSB when cocultured with tumor cells. Conclusions Our results demonstrate that colonic stem cells from NAT in the tumor microenvironment undergo changes at the molecular level, and these molecular characteristics can be maintained in vitro, which can induce fibrosis and an inflammatory response. Video Abstract |
| first_indexed | 2024-03-12T17:06:55Z |
| format | Article |
| id | doaj.art-39c0e4f54c514c33ba63222fc63a1e7d |
| institution | Directory Open Access Journal |
| issn | 1478-811X |
| language | English |
| last_indexed | 2024-03-12T17:06:55Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj.art-39c0e4f54c514c33ba63222fc63a1e7d2023-08-06T11:20:39ZengBMCCell Communication and Signaling1478-811X2023-08-0121111310.1186/s12964-023-01140-1Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancerYuanyuan Zhao0Mengmeng Guo1Fuqiang Zhao2Qian Liu3Xia Wang4School of Pharmaceutical Sciences, Tsinghua UniversitySchool of Pharmaceutical Sciences, Tsinghua UniversityDepartment of Colorectal Surgery, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeDepartment of Colorectal Surgery, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeSchool of Pharmaceutical Sciences, Tsinghua UniversityAbstract Background In colorectal cancer (CRC), the normal tissue adjacent to tumor (NAT) communicates actively with the tumor. Adult stem cells from the colon play a crucial role in the development of the colonic epithelium. In the tumor microenvironment, however, it is unclear what changes have occurred in colonic stem cells derived from NAT. Methods Using an intestinal stem cell culture system, we cultured colonic cells from NAT and paired CRC tissue, as well as cells from healthy tissue (HLT). Clonogenicity and differentiation ability were used to compare the function of clones from NAT, HLT and CRC tissues. RNA high-throughput sequencing of these clones was used to identify the molecular characteristics of NAT-derived clones. Coculture of clones from HLT and CRC was used to assess molecular changes. Results We found that the morphological characteristics, clonogenic ability, and differentiation ability of NAT-derived clones were consistent with those of HLT-derived clones. However, NAT-derived clones changed at the molecular level. A number of genes were specifically activated in NAT. NAT-derived clones enriched pathways related to inflammation and fibrosis, including epithelial mesenchymal transition (EMT) pathway and TGF-beta signaling pathway. Our results also confirmed that NAT-derived clones could recruit fibroblasts in mice. In addition, HLT-derived clones showed high expression of FOSB when cocultured with tumor cells. Conclusions Our results demonstrate that colonic stem cells from NAT in the tumor microenvironment undergo changes at the molecular level, and these molecular characteristics can be maintained in vitro, which can induce fibrosis and an inflammatory response. Video Abstracthttps://doi.org/10.1186/s12964-023-01140-1Stem cellsColorectal cancerMicroenvironmentFibrosisInflammation |
| spellingShingle | Yuanyuan Zhao Mengmeng Guo Fuqiang Zhao Qian Liu Xia Wang Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer Cell Communication and Signaling Stem cells Colorectal cancer Microenvironment Fibrosis Inflammation |
| title | Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer |
| title_full | Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer |
| title_fullStr | Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer |
| title_full_unstemmed | Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer |
| title_short | Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer |
| title_sort | colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer |
| topic | Stem cells Colorectal cancer Microenvironment Fibrosis Inflammation |
| url | https://doi.org/10.1186/s12964-023-01140-1 |
| work_keys_str_mv | AT yuanyuanzhao colonicstemcellsfromnormaltissuesadjacenttotumordriveinflammationandfibrosisincolorectalcancer AT mengmengguo colonicstemcellsfromnormaltissuesadjacenttotumordriveinflammationandfibrosisincolorectalcancer AT fuqiangzhao colonicstemcellsfromnormaltissuesadjacenttotumordriveinflammationandfibrosisincolorectalcancer AT qianliu colonicstemcellsfromnormaltissuesadjacenttotumordriveinflammationandfibrosisincolorectalcancer AT xiawang colonicstemcellsfromnormaltissuesadjacenttotumordriveinflammationandfibrosisincolorectalcancer |